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Peer-reviewedCC BY-NC-NDNature Communications

A copper-dependent redox-based hydrogen peroxide perception in plants

  • Nobuaki Ishihama1,2,3
  • Yohta Fukuda4,5,6,7
  • Yumiko Shirano8,9,10
  • Kazuhiro J. Fujimoto8,2,3
  • Kaori Takizawa1,2,3
  • Ryoko Hiroyama1,9,10
  • Hiroki Ito11,12,13
  • Mayumi Nishimura11,12,13
  • Takeshi Yanai8,9,10
  • Tsuyoshi Inoue A14,4,5,6,7
  • Ken Shirasu15,1,2,3
  • Anuphon Laohavisit16,9,10
16 affiliations
  1. 1RIKEN Center for Sustainable Resource Science, Yokohama, Japan
  2. 2Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science
  3. 3Yokohama, Kanagawa, 230-0045, Japan
  4. 4Graduate School of Pharmaceutical Sciences, The University of Osaka, Suita, Japan
  5. 5Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives (OTRI), The University of Osaka, Suita, Japan
  6. 6Graduate School of Pharmaceutical Sciences, Osaka University
  7. 7Suita, Osaka, 565-0871, Japan
  8. 8Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan
  9. 9Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University
  10. 10Nagoya, Aichi, 464-8601, Japan
  11. 11THERS Technical Center, Nagoya University, Nagoya, Japan
  12. 12Nagoya, Aichi, 6-8603, Japan
  13. 13THERS Technical Center, Nagoya University
  14. 14Center for Infectious Disease Education and Research (CiDER), The University of Osaka, Suita, Japan
  15. 15Department of Biological Science, Graduate School of Science, The University of Tokyo, Bunkyo, Japan
  16. 16Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Japan. [email protected]
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Abstract

Redox-related molecules, such as quinones and reactive oxygen species (ROS), are important signaling molecules for all living organisms. A plant-specific leucine rich-repeat receptor-like kinase (LRR-RLK) CANNOT RESPOND TO DMBQ 1 (CARD1), also known as HYDROGEN-PEROXIDE-INDUCED Ca2+ INCREASES (HPCA1), perceives both quinones and ROS, but knowledge on key structural features is unknown. Here, we determine the structure of the CARD1 ectodomain and uncover its unique features. Structural studies, coupled with genetics and biochemical analysis, demonstrated that previously identified unique cysteine residues are not essential for signal perception in CARD1. Interestingly, CARD1 harbors a copper ion on the surface of the ectodomain via histidine-coordination that is crucial for hydrogen peroxide signaling. Our work reports the first copper-dependent redox receptor and highlights a unique perspective between a receptor and non-peptide stimuli during their perception.

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Cite

BibTeXRIS
BibTeX
@article{ishihama2026copperdependent,
  title = {{A copper-dependent redox-based hydrogen peroxide perception in plants}},
  author = {Nobuaki Ishihama and Yohta Fukuda and Yumiko Shirano and Kazuhiro J. Fujimoto and Kaori Takizawa and Ryoko Hiroyama and Hiroki Ito and Mayumi Nishimura and Takeshi Yanai and Tsuyoshi Inoue A and Ken Shirasu and Anuphon Laohavisit},
  journal = {Nature Communications},
  year = {2026},
  month = may,
  doi = {10.1038/s41467-026-72573-8},
  url = {https://doi.org/10.1038/s41467-026-72573-8},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - A copper-dependent redox-based hydrogen peroxide perception in plants
AU  - Nobuaki Ishihama
AU  - Yohta Fukuda
AU  - Yumiko Shirano
AU  - Kazuhiro J. Fujimoto
AU  - Kaori Takizawa
AU  - Ryoko Hiroyama
AU  - Hiroki Ito
AU  - Mayumi Nishimura
AU  - Takeshi Yanai
AU  - Tsuyoshi Inoue A
AU  - Ken Shirasu
AU  - Anuphon Laohavisit
PY  - 2026
DA  - 2026/05/18
JO  - Nature Communications
DO  - 10.1038/s41467-026-72573-8
UR  - https://doi.org/10.1038/s41467-026-72573-8
AB  - Redox-related molecules, such as quinones and reactive oxygen species (ROS), are important signaling molecules for all living organisms. A plant-specific leucine rich-repeat receptor-like kinase (LRR-RLK) CANNOT RESPOND TO DMBQ 1 (CARD1), also known as HYDROGEN-PEROXIDE-INDUCED Ca2+ INCREASES (HPCA1), perceives both quinones and ROS, but knowledge on key structural features is unknown. Here, we determine the structure of the CARD1 ectodomain and uncover its unique features. Structural studies, coupled with genetics and biochemical analysis, demonstrated that previously identified unique cysteine residues are not essential for signal perception in CARD1. Interestingly, CARD1 harbors a copper ion on the surface of the ectodomain via histidine-coordination that is crucial for hydrogen peroxide signaling. Our work reports the first copper-dependent redox receptor and highlights a unique perspective between a receptor and non-peptide stimuli during their perception.
SN  - 2041-1723
ER  -