Taxogenomic and Physiological Characterization of Halomonas shizuishanensis sp. nov. Reveals Osmotic-Adaptation Traits and an Ammonium-Utilization Phenotype
4 affiliations
- 1East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, China
- 2School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China
- 3Department of Environmental Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
- 4College of Life Science and Technology, Tarim University, 1487 East Talimu Avenue, Alar 843300, China
Abstract
Extreme saline–alkaline environments impose simultaneous osmotic and nitrogen stresses, yet the traits that enable microbial persistence and nitrogen utilization under these conditions remain incompletely understood. Here, we isolated and characterized a novel bacterium, Halomonas shizuishanensis DWK9T, from a saline–alkaline soil (salinity 5.75 g/kg, pH 8.35) in Shizuishan City, Ningxia, China, using a polyphasic taxonomic and genomic approach. Strain DWK9T grew at 0–20% (w/v) NaCl and pH 7.0–10.0. Although its 16S ribosomal RNA gene shared 98.4% similarity with H. lutescens Q1UTT, phylogenomic analysis and genome-based indices, including average nucleotide identity (88.12%) and digital DNA-DNA hybridization (32.5%), supported its classification as a distinct species. Genome analysis identified a complete ectABC cluster and genes annotated for ammonium assimilation and nitrate/nitrite metabolism, including glnA, gdhA, nasA, narGK, and nirBD. Physiological experiments showed that extracellular ammonium nitrogen concentrations decreased in inoculated cultures relative to the corresponding sterile control, concomitantly with bacterial growth, while nitrite nitrogen accumulated only to a limited extent. These findings provide genomic and physiological observations relevant to osmotic adaptation and inorganic-nitrogen dynamics in a halotolerant bacterium from a saline–alkaline ecosystem.
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BibTeX
@article{sun2026taxogenomic,
title = {{Taxogenomic and Physiological Characterization of Halomonas shizuishanensis sp. nov. Reveals Osmotic-Adaptation Traits and an Ammonium-Utilization Phenotype}},
author = {Zhen Sun and Beili Sun and Zhichao Deng and Jinchang Han and Yikang Wu and Jinqiu Wang and Pengcheng Gao and Yiming Li and Kai Zhou and Yuxing Wei and Yan Li and Zongli Yao and Qifang Lai},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27188335},
url = {https://www.mdpi.com/1422-0067/27/18/8335},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Taxogenomic and Physiological Characterization of Halomonas shizuishanensis sp. nov. Reveals Osmotic-Adaptation Traits and an Ammonium-Utilization Phenotype
AU - Zhen Sun
AU - Beili Sun
AU - Zhichao Deng
AU - Jinchang Han
AU - Yikang Wu
AU - Jinqiu Wang
AU - Pengcheng Gao
AU - Yiming Li
AU - Kai Zhou
AU - Yuxing Wei
AU - Yan Li
AU - Zongli Yao
AU - Qifang Lai
PY - 2026
DA - 2026/09/19
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27188335
UR - https://www.mdpi.com/1422-0067/27/18/8335
AB - Extreme saline–alkaline environments impose simultaneous osmotic and nitrogen stresses, yet the traits that enable microbial persistence and nitrogen utilization under these conditions remain incompletely understood. Here, we isolated and characterized a novel bacterium, Halomonas shizuishanensis DWK9T, from a saline–alkaline soil (salinity 5.75 g/kg, pH 8.35) in Shizuishan City, Ningxia, China, using a polyphasic taxonomic and genomic approach. Strain DWK9T grew at 0–20% (w/v) NaCl and pH 7.0–10.0. Although its 16S ribosomal RNA gene shared 98.4% similarity with H. lutescens Q1UTT, phylogenomic analysis and genome-based indices, including average nucleotide identity (88.12%) and digital DNA-DNA hybridization (32.5%), supported its classification as a distinct species. Genome analysis identified a complete ectABC cluster and genes annotated for ammonium assimilation and nitrate/nitrite metabolism, including glnA, gdhA, nasA, narGK, and nirBD. Physiological experiments showed that extracellular ammonium nitrogen concentrations decreased in inoculated cultures relative to the corresponding sterile control, concomitantly with bacterial growth, while nitrite nitrogen accumulated only to a limited extent. These findings provide genomic and physiological observations relevant to osmotic adaptation and inorganic-nitrogen dynamics in a halotolerant bacterium from a saline–alkaline ecosystem.
SN - 1422-0067
ER -