Multifunctional marine polymers composite supplemented with Artemisia absinthium emulsion for improving chronic wound healing: an in vitro study
Abstract
The aim of this study was to develop a 3D multifunctional porous composite based on marine polymers, fish collagen (COL) extracted from Dicentrarchus labrax skin and sulfated polysaccharides (SP) extracted from Cladophora vagabunda seaweed, supplemented with wormwood ( Artemisia absinthium ) emulsion (WE) for improving the healing of chronic wounds. Characterization of the submicron WE based on wormwood hydrolate showed spherical droplets by TEM, Z-average diameter of 21.31 nm and negative zeta potential determined by DLS and antimicrobial activity against Staphylococcus aureus. The 3D COL-SP-WE composite revealed porous morphology by SEM, and a gradual stress increase characteristic for a soft network capable of sustaining medium loads before deformation. The composite had good cytocompatibility in human HaCaT keratinocytes culture. In addition, supplementation of marine composite COL-SP with WE demonstrated higher decrease of the intracellular ROS production in oxidative stressed HaCaT cells, reduced level of pro-inflammatory cytokines in inflamed human THP-1-derived macrophage cells, and downregulation of matrix metalloproteinases mRNAs expression and secretion level in a HaCaT/THP-1 co-culture. However, WE supplementation did not significantly improve HaCaT migration rate in a scratch assay. All these results highlighted multifunctionality of marine composite COL-SP supplemented with WE and recommend further in vivo testing in relevant wound healing models.
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BibTeX
@article{gasparpintiliescu2026multifunctional,
title = {{Multifunctional marine polymers composite supplemented with Artemisia absinthium emulsion for improving chronic wound healing: an in vitro study}},
author = {Alexandra Gaspar-Pintiliescu and Ana-Maria Seciu-Grama and Andreea Plangu and Vasile Sorin Manoiu and Maria Râpă and Andrada Serafim and Justinian Tomescu and Oana Craciunescu},
journal = {Scientific Reports},
year = {2026},
month = oct,
doi = {10.1038/s41598-026-73804-0},
url = {https://www.nature.com/articles/s41598-026-73804-0},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Multifunctional marine polymers composite supplemented with Artemisia absinthium emulsion for improving chronic wound healing: an in vitro study
AU - Alexandra Gaspar-Pintiliescu
AU - Ana-Maria Seciu-Grama
AU - Andreea Plangu
AU - Vasile Sorin Manoiu
AU - Maria Râpă
AU - Andrada Serafim
AU - Justinian Tomescu
AU - Oana Craciunescu
PY - 2026
DA - 2026/10/01
JO - Scientific Reports
DO - 10.1038/s41598-026-73804-0
UR - https://www.nature.com/articles/s41598-026-73804-0
AB - The aim of this study was to develop a 3D multifunctional porous composite based on marine polymers, fish collagen (COL) extracted from Dicentrarchus labrax skin and sulfated polysaccharides (SP) extracted from Cladophora vagabunda seaweed, supplemented with wormwood ( Artemisia absinthium ) emulsion (WE) for improving the healing of chronic wounds. Characterization of the submicron WE based on wormwood hydrolate showed spherical droplets by TEM, Z-average diameter of 21.31 nm and negative zeta potential determined by DLS and antimicrobial activity against Staphylococcus aureus. The 3D COL-SP-WE composite revealed porous morphology by SEM, and a gradual stress increase characteristic for a soft network capable of sustaining medium loads before deformation. The composite had good cytocompatibility in human HaCaT keratinocytes culture. In addition, supplementation of marine composite COL-SP with WE demonstrated higher decrease of the intracellular ROS production in oxidative stressed HaCaT cells, reduced level of pro-inflammatory cytokines in inflamed human THP-1-derived macrophage cells, and downregulation of matrix metalloproteinases mRNAs expression and secretion level in a HaCaT/THP-1 co-culture. However, WE supplementation did not significantly improve HaCaT migration rate in a scratch assay. All these results highlighted multifunctionality of marine composite COL-SP supplemented with WE and recommend further in vivo testing in relevant wound healing models.
SN - 2045-2322
ER -