Vascularized Organoids Emerge from a Heart-Foregut-like Intermediate Recapitulating Early Human Development

Abstract

During early cardiogenesis, the heart regions lie in close contact with foregut endoderm, which supplies essential nutrients and signaling cues to orchestrate primitive heart tube assembly. Here, we show that self-organized heart/foregut like-structures emerge from human pluripotent stem cells (hPSCs). The initial heart/foregut-like structures, termed the Seed, are encased by surrounding supportive cells termed Soil, which provide a matrix microenvironment; the Seed progressively develops into chambered human cardiac organoids (hCOs) containing cardiomyocytes (CMs), epicardial cells, cardiac fibroblasts, and endothelial cells. Optimization of the induction system further enhanced hCO maturation and vascularization. Therefore, this Soil-Seed system provides a model to dissect human early heart/foregut self-organization.

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BibTeX RIS

BibTeX

@misc{wang2026vascularized,
  title = {{Vascularized Organoids Emerge from a Heart-Foregut-like Intermediate Recapitulating Early Human Development}},
  author = {W. Wang and Q. He and Z. Xiong and H. Zhang and Q. Meng and X. Su and Y. Guo and Y. Fan and L. Y. Yang and X. Liu and X. Bian and G. Lu and W.-Y. Chan},
  howpublished = {bioRxiv},
  year = {2026},
  month = oct,
  doi = {10.64898/2026.10.21.758395},
  url = {https://www.biorxiv.org/content/10.64898/2026.10.21.758395v1},
  note = {CC BY-NC-ND},
}

RIS

TY  - UNPB
TI  - Vascularized Organoids Emerge from a Heart-Foregut-like Intermediate Recapitulating Early Human Development
AU  - W. Wang
AU  - Q. He
AU  - Z. Xiong
AU  - H. Zhang
AU  - Q. Meng
AU  - X. Su
AU  - Y. Guo
AU  - Y. Fan
AU  - L. Y. Yang
AU  - X. Liu
AU  - X. Bian
AU  - G. Lu
AU  - W.-Y. Chan
PY  - 2026
DA  - 2026/10/21
PB  - bioRxiv
DO  - 10.64898/2026.10.21.758395
UR  - https://www.biorxiv.org/content/10.64898/2026.10.21.758395v1
AB  - During early cardiogenesis, the heart regions lie in close contact with foregut endoderm, which supplies essential nutrients and signaling cues to orchestrate primitive heart tube assembly. Here, we show that self-organized heart/foregut like-structures emerge from human pluripotent stem cells (hPSCs). The initial heart/foregut-like structures, termed the Seed, are encased by surrounding supportive cells termed Soil, which provide a matrix microenvironment; the Seed progressively develops into chambered human cardiac organoids (hCOs) containing cardiomyocytes (CMs), epicardial cells, cardiac fibroblasts, and endothelial cells. Optimization of the induction system further enhanced hCO maturation and vascularization. Therefore, this Soil-Seed system provides a model to dissect human early heart/foregut self-organization.
ER  -

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