Skip to content
DraconiticResearch
Search Sources API
201,683 studies31 sourcesUpdated 37 min ago
PreprintCC BY-NC-NDChemRxiv

Direct large-volume injection with liquid chromatography/high-resolution mass spectrometry for screening of bacterial growth substrates in tap water

  • Yutaro Uehara1,2
  • Ikuro Kasuga3,2
  • Futoshi Kurisu1,2
  1. 1Graduate School of Engineering
  2. 2University of Tokyo
  3. 3Research Center for Advanced Science and Technology
Open at sourcePDFDOICite

Abstract

Solid-phase extraction (SPE) is widely used for sample pretreatment before liquid chromatographymass spectrometry (LC/MS). However, it may constrain the comprehensiveness of non-targeted LC/MS analysis, as a fraction of dissolved organic matter (DOM) cannot be recovered by SPE. In this study, direct large-volume injection (dLVI) without SPE was applied to explore potential growth substrates that support the proliferation of Pseudomonas aeruginosa, an opportunistic pathogen found in tap water. Non-targeted analysis (NTA) detected 2,497 DOM components, of which 65 were screened as putative substrates for P. aeruginosa. Notably, none of the substrate candidates detected by dLVI were screened in the SPE analysis. Furthermore, the distributions of molecular descriptors, including unsaturation and oxidation states, differed significantly between these methods. These results highlight their methodological complementarity. LC/MS/MS analysis and co-chromatography identified one compound, C9H17NO3, as 7-keto-8-aminopelargonic acid (KAPA), an intermediate in biotin synthesis. Although KAPA did not support P. aeruginosa proliferation, the results indicated that it may contribute to microbial proliferation. This study underscores the potential of dLVI to reveal previously inaccessible DOM fractions.

Related

  1. Hydrophilic interaction chromatography and tandem mass spectrometry for the characterization of methylated oligoribonucleotides
    Khaoula Adouairi, Carole Farre, Carole Chaix +2
    Journal of Pharmaceutical and Biomedical AnalysisPeer-reviewedCC BY-NC 4.0Full text1 citedProteomics & metabolomicsGeneral biologyHAL
  2. Linkage between Quaternary Structure and Subunit Sequencing of Zebra Finch Hemoglobin via Hyphenation of Size Exclusion Chromatography with Native Top-Down Mass Spectrometry
    Léa Letissier, Corentin Beaumal, François Criscuolo +5
    Journal of The American Society for Mass SpectrometryPeer-reviewedCC BY 4.0Full textGeneral biochemistryGeneral biologyHAL
  3. A Liquid Chromatography-Mass Spectrometry Method to Quantify Total Coenzyme A Concentration and Isotopic Labeling.
    Amelia L Taylor, Nathaniel W Snyder, Caroline R Bartman
    Journal of the American Society for Mass SpectrometryPeer-reviewedCC BY-NC-NDFull textMolecular biology & gene regulationEurope PMC
  4. Optimizing Oxylipin Analysis with Liquid Chromatography Mass Spectrometry through Bio-Inert Systems and Ion Funnel Adjustments
    J. T. Shuster, L. Wu, J. Mill +9
    bioRxivPreprintCC BYFull textMolecular biology & gene regulationbioRxiv
  5. High-throughput droplet screening of filamentous fungi on solid substrates
    Chiara Leal Alves, Jeremy Z. Wu, Elham Khanjani +7
    RSC Chemical BiologyPeer-reviewedCC BY-NC 3.0Full textMolecular biology & gene regulationPMC

Cite

BibTeXRIS
BibTeX
@misc{uehara2026direct,
  title = {{Direct large-volume injection with liquid chromatography/high-resolution mass spectrometry for screening of bacterial growth substrates in tap water}},
  author = {Yutaro Uehara and Ikuro Kasuga and Futoshi Kurisu},
  howpublished = {ChemRxiv},
  year = {2026},
  month = oct,
  doi = {10.26434/chemrxiv.15011099/v1},
  url = {https://chemrxiv.org/doi/full/10.26434/chemrxiv.15011099/v1},
  note = {CC BY-NC-ND},
}
RIS
TY  - UNPB
TI  - Direct large-volume injection with liquid chromatography/high-resolution mass spectrometry for screening of bacterial growth substrates in tap water
AU  - Yutaro Uehara
AU  - Ikuro Kasuga
AU  - Futoshi Kurisu
PY  - 2026
DA  - 2026/10/27
PB  - ChemRxiv
DO  - 10.26434/chemrxiv.15011099/v1
UR  - https://chemrxiv.org/doi/full/10.26434/chemrxiv.15011099/v1
AB  - Solid-phase extraction (SPE) is widely used for sample pretreatment before liquid chromatographymass spectrometry (LC/MS). However, it may constrain the comprehensiveness of non-targeted LC/MS analysis, as a fraction of dissolved organic matter (DOM) cannot be recovered by SPE. In this study, direct large-volume injection (dLVI) without SPE was applied to explore potential growth substrates that support the proliferation of Pseudomonas aeruginosa, an opportunistic pathogen found in tap water. Non-targeted analysis (NTA) detected 2,497 DOM components, of which 65 were screened as putative substrates for P. aeruginosa. Notably, none of the substrate candidates detected by dLVI were screened in the SPE analysis. Furthermore, the distributions of molecular descriptors, including unsaturation and oxidation states, differed significantly between these methods. These results highlight their methodological complementarity. LC/MS/MS analysis and co-chromatography identified one compound, C9H17NO3, as 7-keto-8-aminopelargonic acid (KAPA), an intermediate in biotin synthesis. Although KAPA did not support P. aeruginosa proliferation, the results indicated that it may contribute to microbial proliferation. This study underscores the potential of dLVI to reveal previously inaccessible DOM fractions.
ER  -