Microfluidic sperm separation for intracytoplasmic sperm injection yields similar blastocyst rates compared with density gradient, however, may be of benefit for male factor infertility: a randomized sibling oocyte study.
5 affiliations
- 1Department of Obstetrics and Gynecology, Northwell Health Fertility, Manhasset, New York.
- 2Department of Obstetrics and Gynecology, NYU Langone Reproductive Specialists of New York, Mineola, New York.
- 3Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, Pennsylvania.
- 4Department of Obstetrics and Gynecology, NYU Langone Long Island School of Medicine, Mineola, New York.
- 5Department of Foundations of Medicine, NYU Langone Long Island School of Medicine, Mineola, New York.
Abstract
Objective
To study whether microfluidic sperm separation in intracytoplasmic sperm injection cycles provides any benefit over density gradient sperm separation for good-quality embryo formation.Design
Randomized sibling oocyte study.Subjects
Patients undergoing intracytoplasmic sperm injection.Intervention
Microfluidic sperm separation vs. density gradient sperm separation.Main outcome measures
Primary outcomes were percentage of good-quality blastocyst formation. Secondary outcomes included sperm parameters after sperm separation, including percent motility, grade of forward progression, fertilization rates, blastocyst formation rates, and ploidy rates in the event that preimplantation testing for aneuploidy was performed. Additionally, subanalysis on those with male factor infertility was performed, evaluating primary and secondary outcomes above.Results
A total of 34 cycles enrolled into the study, yielding 456 oocytes, 231 randomized to microfluidics and 225 to density gradient (DG). Average microfluidic fertilization rate was 76.1% ± 19.2% and 72.4% ± 26.6% for DG. Microfluidic blastocyst formation rate was 58.4% ± 31.9% compared with 58.7% ± 30.5% for DG. Good-quality blastocyst formation rate for microfluidics was 51.4% ± 29.7% and for DG was 51.7% ± 30.9%. Euploid rates were 43.8% ± 35.5% in the microfluidics cohort and 33.7% ± 33.3% in the DG cohort. Subanalysis of cycles with male factor infertility revealed good-quality blastocyst formation rate was 45.9% ± 30.9% after microfluidics and 55.1% ± 29.2% for DG. Subanalysis of cycles with male factor infertility revealed an euploid rate of 50.0% ± 37.6% after microfluidics compared with 18.4% ± 24.5% for DG.Conclusion
There were similar rates of good-quality embryo formation after intracytoplasmic sperm injection using sperm separated with microfluidics compared with density gradient; however, subanalysis of the male factor infertility group revealed higher euploid rates with microfluidics.Trial registration
This trial was registered in clinicaltrials.gov, ID number NCT04818593. URL: https://clinicaltrials.gov/study/NCT04818593. Date of registration: 3/25/2021. Date of first enrollment: 6/18/2021.
Related
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- Randomized non-inferiority within-patient trial of density gradient processing of sequential ejaculates versus centrifugation-free sperm separation device for ICSI in men with elevated sperm DNA fragmentation- study protocol
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BibTeX
@article{fritz2026microfluidic,
title = {{Microfluidic sperm separation for intracytoplasmic sperm injection yields similar blastocyst rates compared with density gradient, however, may be of benefit for male factor infertility: a randomized sibling oocyte study.}},
author = {Rani Fritz and Alicia Arnott and Jessica Tozour and Linda Sung and Maria Saketos and Satu Kuokkanen and Vasilios Goudas and Anand Rajan},
journal = {F\&S reports},
year = {2026},
month = aug,
doi = {10.1016/j.xfre.2026.08.003},
url = {https://europepmc.org/article/MED/42903651},
note = {CC BY},
}
RIS
TY - JOUR
TI - Microfluidic sperm separation for intracytoplasmic sperm injection yields similar blastocyst rates compared with density gradient, however, may be of benefit for male factor infertility: a randomized sibling oocyte study.
AU - Rani Fritz
AU - Alicia Arnott
AU - Jessica Tozour
AU - Linda Sung
AU - Maria Saketos
AU - Satu Kuokkanen
AU - Vasilios Goudas
AU - Anand Rajan
PY - 2026
DA - 2026/08/18
JO - F&S reports
DO - 10.1016/j.xfre.2026.08.003
UR - https://europepmc.org/article/MED/42903651
AB - Objective To study whether microfluidic sperm separation in intracytoplasmic sperm injection cycles provides any benefit over density gradient sperm separation for good-quality embryo formation.Design Randomized sibling oocyte study.Subjects Patients undergoing intracytoplasmic sperm injection.Intervention Microfluidic sperm separation vs. density gradient sperm separation.Main outcome measures Primary outcomes were percentage of good-quality blastocyst formation. Secondary outcomes included sperm parameters after sperm separation, including percent motility, grade of forward progression, fertilization rates, blastocyst formation rates, and ploidy rates in the event that preimplantation testing for aneuploidy was performed. Additionally, subanalysis on those with male factor infertility was performed, evaluating primary and secondary outcomes above.Results A total of 34 cycles enrolled into the study, yielding 456 oocytes, 231 randomized to microfluidics and 225 to density gradient (DG). Average microfluidic fertilization rate was 76.1% ± 19.2% and 72.4% ± 26.6% for DG. Microfluidic blastocyst formation rate was 58.4% ± 31.9% compared with 58.7% ± 30.5% for DG. Good-quality blastocyst formation rate for microfluidics was 51.4% ± 29.7% and for DG was 51.7% ± 30.9%. Euploid rates were 43.8% ± 35.5% in the microfluidics cohort and 33.7% ± 33.3% in the DG cohort. Subanalysis of cycles with male factor infertility revealed good-quality blastocyst formation rate was 45.9% ± 30.9% after microfluidics and 55.1% ± 29.2% for DG. Subanalysis of cycles with male factor infertility revealed an euploid rate of 50.0% ± 37.6% after microfluidics compared with 18.4% ± 24.5% for DG.Conclusion There were similar rates of good-quality embryo formation after intracytoplasmic sperm injection using sperm separated with microfluidics compared with density gradient; however, subanalysis of the male factor infertility group revealed higher euploid rates with microfluidics.Trial registration This trial was registered in clinicaltrials.gov, ID number NCT04818593. URL: https://clinicaltrials.gov/study/NCT04818593. Date of registration: 3/25/2021. Date of first enrollment: 6/18/2021.
SN - 2666-3341
ER -