Antimicrobial Usage and Presence of Extended-Spectrum β-Lactamase-Producing Enterobacterales in Healthy Chickens From Farms in Grand-Abidjan and Korhogo, Côte d'Ivoire.
7 affiliations
- 1Laboratory of Biotechnology and Agro-Food Valorization UFR of Biological Sciences Universite Peleforo Gon Coulibaly Korhogo Ivory Coast.
- 2Laboratory of Biology and Health UFR of Biosciences Universite Felix Houphouet-Boigny Abidjan Ivory Coast.
- 3Laboratory of Microbiology Biotechnology and Bioinformatics Institut National Polytechnique Felix Houphouet-Boigny Yamoussoukro Ivory Coast.
- 4Department of Parasitology-Mycology Institut Pasteur de Cote d'Ivoire Abidjan Ivory Coast.
- 5Doctoral School of Biology-Environment-Health UFR of Biosciences Universite Felix Houphouet-Boigny Abidjan Ivory Coast.
- 6Laboratory of Biotechnology Agriculture and Biological Resources Valorization UFR of Biosciences Universite Felix Houphouet-Boigny Abidjan Ivory Coast.
- 7Laboratory of Bacteriology-Virology UFR of Medical Sciences Universite Felix Houphouet-Boigny Abidjan Ivory Coast.
Abstract
Background
The global spread of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales in livestock poses critical threats to human and animal health, particularly in low- and middle-income countries. This study investigated the prevalence, antibiotic resistance profiles, and genetic determinants of ESBL-producing Enterobacterales in healthy chickens from poultry farms in Grand-Abidjan and Korhogo, Côte d'Ivoire, alongside antibiotic use practices in the sector.Methods
A semistructured questionnaire assessed veterinary practices across 26 farms, whereas chicken fecal samples from 27 farms were screened for third-generation cephalosporin-resistant Enterobacterales. Phenotypic and genotypic characterization of ESBL producers was performed, along with antimicrobial susceptibility testing against 12 antibiotics.Results
All farmers reported antibiotic use, primarily tetracyclines (76.9%), macrolides (69.2%), polymyxins (61.5%), aminoglycosides (57.7%), and sulfonamides (53.8%), mainly for disease prophylaxis (65.38%) and often without veterinary supervision. ESBL-producing Enterobacterales were detected in 96.3% (26/27) of fecal samples, yielding 90 isolates identified as clinically relevant species: Klebsiella pneumoniae (42.2%, 38/90), Escherichia coli (38.9%, 35/90), and Enterobacter spp. (18.9%, 17/90). Overall, 74.8% (63/90) of isolates were resistant to trimethoprim-sulfamethoxazole and 57.8% to amoxicillin-clavulanic acid, whereas no resistance to carbapenems was observed. Molecular characterization revealed the presence of bla CTX-M, bla SHV, and bla TEM genes, with bla CTX-M being the most prevalent (77.8%, 70/90), followed by bla SHV (54.4%, 49/90) and bla TEM (31.1%, 28/90). These genes were coexpressed in half of all isolates, predominantly in K. pneumoniae and Enterobacter spp.Conclusion
This study highlights the widespread dissemination of ESBL-producing Enterobacterales in poultry farms in Côte d'Ivoire, emphasizing the role of poultry as a reservoir for resistant bacteria and underscoring the urgent need for integrated surveillance and antimicrobial stewardship within a One Health framework.
Related
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BibTeX
@article{essoh2026antimicrobial,
title = {{Antimicrobial Usage and Presence of Extended-Spectrum β-Lactamase-Producing Enterobacterales in Healthy Chickens From Farms in Grand-Abidjan and Korhogo, Côte d'Ivoire.}},
author = {Christiane You Essoh and Gilles Alex Pakora and Daouda Toure and Inès Cinthia A Yebouet and Bérenger Aristide A Ako and Jemima Arlette Ayebie and Naomie Dali and Sinon-Pierre Assanvo N Guetta and Adèle Kakou-N Douba},
journal = {International journal of microbiology},
year = {2026},
month = oct,
doi = {10.1155/ijm/7418124},
url = {https://europepmc.org/article/MED/42910201},
note = {CC BY},
}
RIS
TY - JOUR
TI - Antimicrobial Usage and Presence of Extended-Spectrum β-Lactamase-Producing Enterobacterales in Healthy Chickens From Farms in Grand-Abidjan and Korhogo, Côte d'Ivoire.
AU - Christiane You Essoh
AU - Gilles Alex Pakora
AU - Daouda Toure
AU - Inès Cinthia A Yebouet
AU - Bérenger Aristide A Ako
AU - Jemima Arlette Ayebie
AU - Naomie Dali
AU - Sinon-Pierre Assanvo N Guetta
AU - Adèle Kakou-N Douba
PY - 2026
DA - 2026/10/21
JO - International journal of microbiology
DO - 10.1155/ijm/7418124
UR - https://europepmc.org/article/MED/42910201
AB - Background The global spread of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales in livestock poses critical threats to human and animal health, particularly in low- and middle-income countries. This study investigated the prevalence, antibiotic resistance profiles, and genetic determinants of ESBL-producing Enterobacterales in healthy chickens from poultry farms in Grand-Abidjan and Korhogo, Côte d'Ivoire, alongside antibiotic use practices in the sector.Methods A semistructured questionnaire assessed veterinary practices across 26 farms, whereas chicken fecal samples from 27 farms were screened for third-generation cephalosporin-resistant Enterobacterales. Phenotypic and genotypic characterization of ESBL producers was performed, along with antimicrobial susceptibility testing against 12 antibiotics.Results All farmers reported antibiotic use, primarily tetracyclines (76.9%), macrolides (69.2%), polymyxins (61.5%), aminoglycosides (57.7%), and sulfonamides (53.8%), mainly for disease prophylaxis (65.38%) and often without veterinary supervision. ESBL-producing Enterobacterales were detected in 96.3% (26/27) of fecal samples, yielding 90 isolates identified as clinically relevant species: Klebsiella pneumoniae (42.2%, 38/90), Escherichia coli (38.9%, 35/90), and Enterobacter spp. (18.9%, 17/90). Overall, 74.8% (63/90) of isolates were resistant to trimethoprim-sulfamethoxazole and 57.8% to amoxicillin-clavulanic acid, whereas no resistance to carbapenems was observed. Molecular characterization revealed the presence of bla CTX-M, bla SHV, and bla TEM genes, with bla CTX-M being the most prevalent (77.8%, 70/90), followed by bla SHV (54.4%, 49/90) and bla TEM (31.1%, 28/90). These genes were coexpressed in half of all isolates, predominantly in K. pneumoniae and Enterobacter spp.Conclusion This study highlights the widespread dissemination of ESBL-producing Enterobacterales in poultry farms in Côte d'Ivoire, emphasizing the role of poultry as a reservoir for resistant bacteria and underscoring the urgent need for integrated surveillance and antimicrobial stewardship within a One Health framework.
SN - 1687-918X
SN - 1687-9198
ER -