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PreprintCC BY-NC 4.0HAL

Live-Imaging of Molecular Processes with Real-Time Fluorescence Microscopy

  • Perrine Revoil
  • Jérôme Rech
  • Jean-Yves Bouet
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Abstract

Here, we present a comprehensive methodology for real-time visualization of fluorescently tagged target proteins in living bacteria. We detail the use of micro-fabricated devices, such as agarose pads and microfluidic systems, to sustain cell viability and enable precise environmental control, ensuring accurate and reproducible imaging conditions. The chapter covers sample preparation, microscope setup, image acquisition, and the analysis of fluorescence dynamics at the single-cell level.

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Cite

BibTeXRIS
BibTeX
@misc{revoil2026liveimaging,
  title = {{Live-Imaging of Molecular Processes with Real-Time Fluorescence Microscopy}},
  author = {Perrine Revoil and Jérôme Rech and Jean-Yves Bouet},
  howpublished = {HAL},
  year = {2026},
  month = oct,
  url = {https://hal.science/hal-05064515v2},
  note = {CC BY-NC 4.0},
}
RIS
TY  - UNPB
TI  - Live-Imaging of Molecular Processes with Real-Time Fluorescence Microscopy
AU  - Perrine Revoil
AU  - Jérôme Rech
AU  - Jean-Yves Bouet
PY  - 2026
DA  - 2026/10/13
PB  - HAL
UR  - https://hal.science/hal-05064515v2
AB  - Here, we present a comprehensive methodology for real-time visualization of fluorescently tagged target proteins in living bacteria. We detail the use of micro-fabricated devices, such as agarose pads and microfluidic systems, to sustain cell viability and enable precise environmental control, ensuring accurate and reproducible imaging conditions. The chapter covers sample preparation, microscope setup, image acquisition, and the analysis of fluorescence dynamics at the single-cell level.
ER  -