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Peer-reviewedCC BYFrontiers in medicine

Pathogen-targeted quick multiplex PCR identification of Chlamydia psittaci and Haemophilus influenzae coinfection in pneumonia: a case report.

  • Ying Yuan1
  • Jianhong Wang1
  • Ning Feng1
  • Fen Wu1
  • Xiao Dong2
  • Youxin Xu1
  • Shunfeng Zhao1
  • Tingzhao Tan3
  1. 1Department of Clinical Laboratory, Liaocheng Third People's Hospital, Liaocheng, China.
  2. 2Department of Critical Care Medicine, Liaocheng Third People's Hospital, Liaocheng, China.
  3. 3Department of Medical Oncology, Liaocheng Tumor Hospital, Liaocheng, China.
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Abstract

This study describes a rare case of pneumonia induced by coinfection with Chlamydia psittaci (C. psittaci) and Haemophilus influenzae (H. influenzae). The patient had a history of chronic nephritis and long-term pigeon breeding. The initial manifestation was slurred speech, followed by rapid progression to high fever and respiratory failure. Chest computed tomography revealed bilateral interstitial pneumonia, predominantly in the right lung. Pathogen-targeted quick multiplex polymerase chain reaction (pt-qPCR) confirmed the pathogens as C. psittaci and H. influenzae, and metagenomic next-generation sequencing (mNGS) further verified consistent results. After 7-days of combination therapy with doxycycline and azithromycin, the treatment was switched to doxycycline combined with moxifloxacin for an additional 8-days. The patient achieved complete recovery and was discharged from the hospital. This case suggests that pt-qPCR may facilitate early identification of mixed infections in patients with severe pneumonia and a history of avian exposure, thereby providing valuable information to guide clinical decision-making.

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Cite

BibTeXRIS
BibTeX
@article{yuan2026pathogentargeted,
  title = {{Pathogen-targeted quick multiplex PCR identification of Chlamydia psittaci and Haemophilus influenzae coinfection in pneumonia: a case report.}},
  author = {Ying Yuan and Jianhong Wang and Ning Feng and Fen Wu and Xiao Dong and Youxin Xu and Shunfeng Zhao and Tingzhao Tan},
  journal = {Frontiers in medicine},
  year = {2026},
  month = oct,
  doi = {10.3389/fmed.2026.1890644},
  url = {https://europepmc.org/article/MED/42898413},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Pathogen-targeted quick multiplex PCR identification of Chlamydia psittaci and Haemophilus influenzae coinfection in pneumonia: a case report.
AU  - Ying Yuan
AU  - Jianhong Wang
AU  - Ning Feng
AU  - Fen Wu
AU  - Xiao Dong
AU  - Youxin Xu
AU  - Shunfeng Zhao
AU  - Tingzhao Tan
PY  - 2026
DA  - 2026/10/05
JO  - Frontiers in medicine
DO  - 10.3389/fmed.2026.1890644
UR  - https://europepmc.org/article/MED/42898413
AB  - This study describes a rare case of pneumonia induced by coinfection with Chlamydia psittaci (C. psittaci) and Haemophilus influenzae (H. influenzae). The patient had a history of chronic nephritis and long-term pigeon breeding. The initial manifestation was slurred speech, followed by rapid progression to high fever and respiratory failure. Chest computed tomography revealed bilateral interstitial pneumonia, predominantly in the right lung. Pathogen-targeted quick multiplex polymerase chain reaction (pt-qPCR) confirmed the pathogens as C. psittaci and H. influenzae, and metagenomic next-generation sequencing (mNGS) further verified consistent results. After 7-days of combination therapy with doxycycline and azithromycin, the treatment was switched to doxycycline combined with moxifloxacin for an additional 8-days. The patient achieved complete recovery and was discharged from the hospital. This case suggests that pt-qPCR may facilitate early identification of mixed infections in patients with severe pneumonia and a history of avian exposure, thereby providing valuable information to guide clinical decision-making.
SN  - 2296-858X
ER  -