Feline-Derived Lactiplantibacillus plantarum-Aloe Vera Polysaccharide Hydrogel as a Topical Control Strategy for Staphylococcus pseudintermedius-Infected Wounds.
4 affiliations
- 1Food Laboratory of Zhongyuan, Luohe 462300, China.
- 2Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.
- 3Institute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing 100083, China.
- 4College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
Abstract
Staphylococcus pseudintermedius (SP) is an important opportunistic pathogen in companion animals, and the increasing prevalence of antimicrobial-resistant strains highlights the need for alternative approaches to local infection control. This study evaluated a topical hydrogel containing feline-derived Lactiplantibacillus plantarum ZYpet-014 and Aloe vera polysaccharides (AVEs) for the treatment of SP-infected wounds. AVEs promoted LP growth and organic-acid production and improved short-term LP viability within the carbomer hydrogel. The LP+AVE-hydrogel inhibited planktonic SP growth and biofilm formation in vitro and caused pronounced morphological disruption of SP cells. Therapeutic efficacy was further evaluated using a full-thickness murine wound model challenged with SP. LP+AVE treatment accelerated wound closure, reaching 95.75% by day 14, while qualitative histological observations suggested improved tissue organization and differences in collagen-associated staining patterns. Treatment was also associated with reduced TNF-α, IL-1β, and IL-6 levels and increased IL-4, IL-10, TGF-β1, and serum VEGF concentrations. In wound tissues, LP+AVE treatment decreased CD80 and increased CD206 immunoreactivity, consistent with a shift in macrophage-associated responses toward a reparative profile. Moreover, 16S rRNA gene sequencing showed reduced relative abundance of Staphylococcus and increased relative abundance of Lactobacillus in treated wounds. Together, these findings demonstrate antibacterial and antibiofilm activities against SP in vitro, accompanied by improved wound healing, modulation of inflammatory responses, and changes in wound microbial composition in vivo, although direct reduction in SP burden in wound tissue remains to be established.
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BibTeX
@article{chen2026felinederived,
title = {{Feline-Derived Lactiplantibacillus plantarum-Aloe Vera Polysaccharide Hydrogel as a Topical Control Strategy for Staphylococcus pseudintermedius-Infected Wounds.}},
author = {Qianqian Chen and Hao Dong and Weiwei Wang and Huakai Wang and Jiahang Yao and Mengyao Ma and Shiqiang Zhu and Longjiao Zhu and Zaihui Du and Zhaofei Xia and Wei Xiong},
journal = {Veterinary sciences},
year = {2026},
month = oct,
doi = {10.3390/vetsci13101126},
url = {https://europepmc.org/article/MED/42944220},
note = {CC BY},
}
RIS
TY - JOUR
TI - Feline-Derived Lactiplantibacillus plantarum-Aloe Vera Polysaccharide Hydrogel as a Topical Control Strategy for Staphylococcus pseudintermedius-Infected Wounds.
AU - Qianqian Chen
AU - Hao Dong
AU - Weiwei Wang
AU - Huakai Wang
AU - Jiahang Yao
AU - Mengyao Ma
AU - Shiqiang Zhu
AU - Longjiao Zhu
AU - Zaihui Du
AU - Zhaofei Xia
AU - Wei Xiong
PY - 2026
DA - 2026/10/21
JO - Veterinary sciences
DO - 10.3390/vetsci13101126
UR - https://europepmc.org/article/MED/42944220
AB - Staphylococcus pseudintermedius (SP) is an important opportunistic pathogen in companion animals, and the increasing prevalence of antimicrobial-resistant strains highlights the need for alternative approaches to local infection control. This study evaluated a topical hydrogel containing feline-derived Lactiplantibacillus plantarum ZYpet-014 and Aloe vera polysaccharides (AVEs) for the treatment of SP-infected wounds. AVEs promoted LP growth and organic-acid production and improved short-term LP viability within the carbomer hydrogel. The LP+AVE-hydrogel inhibited planktonic SP growth and biofilm formation in vitro and caused pronounced morphological disruption of SP cells. Therapeutic efficacy was further evaluated using a full-thickness murine wound model challenged with SP. LP+AVE treatment accelerated wound closure, reaching 95.75% by day 14, while qualitative histological observations suggested improved tissue organization and differences in collagen-associated staining patterns. Treatment was also associated with reduced TNF-α, IL-1β, and IL-6 levels and increased IL-4, IL-10, TGF-β1, and serum VEGF concentrations. In wound tissues, LP+AVE treatment decreased CD80 and increased CD206 immunoreactivity, consistent with a shift in macrophage-associated responses toward a reparative profile. Moreover, 16S rRNA gene sequencing showed reduced relative abundance of Staphylococcus and increased relative abundance of Lactobacillus in treated wounds. Together, these findings demonstrate antibacterial and antibiofilm activities against SP in vitro, accompanied by improved wound healing, modulation of inflammatory responses, and changes in wound microbial composition in vivo, although direct reduction in SP burden in wound tissue remains to be established.
SN - 2306-7381
ER -