CLIC4 inhibits the progression of non-small cell lung cancer by suppressing the MAPK pathway
- 1Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University
- 2Jiangxi Institute of Respiratory Diseases, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University
Abstract
In addition to its involvement in maintaining membrane electrical balance, ion homeostasis, and transmembrane transport, intracellular chloride channel protein 4 (CLIC4) is regulated by signaling molecules such as TGF-β, P53, Myc, and TNFα and is closely associated with apoptosis. Nevertheless, the expression patterns and functional roles of CLIC4 across different cancer types remain inconsistent and are not fully understood. In this study, we initially observed reduced CLIC4 expression in non-small cell lung cancer (NSCLC) through bioinformatics analysis and further validated this downregulation using multidimensional experimental approaches. We established stable NSCLC cell lines with CLIC4 overexpression or knockdown to investigate its effects on cellular proliferation, migration, and invasion. Subsequent RNA sequencing of CLIC4-overexpressing cells revealed that CLIC4 suppressed MAPK-related signaling pathways, which was confirmed by western blotting. These findings suggest that CLIC4 may inhibit the progression of NSCLC by downregulating MAPK signaling, offering potential insights for novel diagnostic and therapeutic strategies in NSCLC.
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BibTeX
@article{gao2026clic4,
title = {{CLIC4 inhibits the progression of non-small cell lung cancer by suppressing the MAPK pathway}},
author = {Pengxiang Gao and Wanjun Lu and Xinyu Tan and Wei Zhang and Xinping Xu},
journal = {Scientific Reports},
year = {2026},
month = jul,
doi = {10.1038/s41598-026-62272-1},
url = {https://doi.org/10.1038/s41598-026-62272-1},
note = {CC BY},
}
RIS
TY - JOUR
TI - CLIC4 inhibits the progression of non-small cell lung cancer by suppressing the MAPK pathway
AU - Pengxiang Gao
AU - Wanjun Lu
AU - Xinyu Tan
AU - Wei Zhang
AU - Xinping Xu
PY - 2026
DA - 2026/07/01
JO - Scientific Reports
DO - 10.1038/s41598-026-62272-1
UR - https://doi.org/10.1038/s41598-026-62272-1
AB - In addition to its involvement in maintaining membrane electrical balance, ion homeostasis, and transmembrane transport, intracellular chloride channel protein 4 (CLIC4) is regulated by signaling molecules such as TGF-β, P53, Myc, and TNFα and is closely associated with apoptosis. Nevertheless, the expression patterns and functional roles of CLIC4 across different cancer types remain inconsistent and are not fully understood. In this study, we initially observed reduced CLIC4 expression in non-small cell lung cancer (NSCLC) through bioinformatics analysis and further validated this downregulation using multidimensional experimental approaches. We established stable NSCLC cell lines with CLIC4 overexpression or knockdown to investigate its effects on cellular proliferation, migration, and invasion. Subsequent RNA sequencing of CLIC4-overexpressing cells revealed that CLIC4 suppressed MAPK-related signaling pathways, which was confirmed by western blotting. These findings suggest that CLIC4 may inhibit the progression of NSCLC by downregulating MAPK signaling, offering potential insights for novel diagnostic and therapeutic strategies in NSCLC.
SN - 2045-2322
ER -