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Peer-reviewedCC BY-NC-ND 4.0STAR Protocols

Protocol for the extraction and purification of shikimic acid from Escherichia coli fermentative media

  • Pedro Silva1
  • Ana C. Afonso1
  • Manuel Simões1
  • Lúcia Santos1
  • Margarida M.S.M. Bastos1
  1. 1LEPABE, ALiCE, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias, 4200-465 Porto, Portugal
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Abstract

Shikimic acid (SA) is a valuable pharmaceutical precursor used in drug synthesis. Here, we present a protocol for isolating and purifying SA from Escherichia coli cultures. We describe steps for combining cell disruption, centrifugation, filtration, preparative anion-exchange chromatography, elution, and lyophilization. We detail procedures for identifying SA-containing fractions by Fourier transform infrared (FTIR) spectroscopy, followed by ethanol-based recrystallization and FTIR characterization of the purified product. This protocol provides an integrated downstream processing strategy for microbial SA production.

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Cite

BibTeXRIS
BibTeX
@article{silva2026protocol,
  title = {{Protocol for the extraction and purification of shikimic acid from Escherichia coli fermentative media}},
  author = {Pedro Silva and Ana C. Afonso and Manuel Simões and Lúcia Santos and Margarida M.S.M. Bastos},
  journal = {STAR Protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104919},
  url = {https://doi.org/10.1016/j.xpro.2026.104919},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - Protocol for the extraction and purification of shikimic acid from Escherichia coli fermentative media
AU  - Pedro Silva
AU  - Ana C. Afonso
AU  - Manuel Simões
AU  - Lúcia Santos
AU  - Margarida M.S.M. Bastos
PY  - 2026
DA  - 2026/10/27
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2026.104919
UR  - https://doi.org/10.1016/j.xpro.2026.104919
AB  - Shikimic acid (SA) is a valuable pharmaceutical precursor used in drug synthesis. Here, we present a protocol for isolating and purifying SA from Escherichia coli cultures. We describe steps for combining cell disruption, centrifugation, filtration, preparative anion-exchange chromatography, elution, and lyophilization. We detail procedures for identifying SA-containing fractions by Fourier transform infrared (FTIR) spectroscopy, followed by ethanol-based recrystallization and FTIR characterization of the purified product. This protocol provides an integrated downstream processing strategy for microbial SA production.
SN  - 2666-1667
ER  -