Skip to content
DraconiticResearch
Search Sources API
197,768 studies31 sourcesUpdated 36 min ago
Peer-reviewedCC BY 4.0Pain Research & Management

Cutaneous Mast Cells Contribute to the Analgesic Effect of Manual Therapy on CCD Rats

  • Jiayang Yu1,2
  • Pingping Sun3,4
  • Dong Xie5
  • Xinyu Zeng2
  • Xili Chang1
  • Min Fang6,7
  • Wuquan Sun1
  • Qingguang Zhu1,7
  • Chongjie Yao1,2
7 affiliations
  1. 1Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine Shanghai 200437 China shutcm.edu.cn
  2. 2School of Acupuncture-moxibustion and Tuina Shanghai University of Traditional Chinese Medicine Shanghai 201203 China shutcm.edu.cn
  3. 3School of Rehabilitation Science Shanghai University of Traditional Chinese Medicine Shanghai 201203 China shutcm.edu.cn
  4. 4Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation Ministry of Education Shanghai 201203 China meb.gov.tr
  5. 5Putuo Hospital Shanghai University of Traditional Chinese Medicine Shanghai 200062 China shutcm.edu.cn
  6. 6Shuguang Hospital Shanghai University of Traditional Chinese Medicine Shanghai 201203 China shutcm.edu.cn
  7. 7Research Institute of Tuina Shanghai Academy of Traditional Chinese Medicine Shanghai 200437 China shutcm.edu.cn
Read full textPDFDOICite

Abstract

Background: Lumbar disc herniation (LDH) commonly induces low back and leg pain. Manual therapy (MT) is effective in relieving such pain clinically, but its mechanism remains unclear. Given that acupoint regions are enriched with mast cells that respond to mechanical stimulation, this study explored whether MT alleviates CCD‐evoked neuropathic pain and its association with the modulation of cutaneous mast cells.

Methods: Sprague‐Dawley (SD) rats were randomized into sham, chronic compression of dorsal root ganglion (CCD), MT, and mast cell inhibition (MCI) groups. After CCD modeling, MT was administered at BL57 starting on day 4 for 14 days, while MCI received acupoint cromolyn sodium injection. Paw withdrawal threshold (PWT) was measured. Mast cell activation was assessed using immunofluorescence and toluidine blue staining. Calcitonin gene‐related peptide (CGRP), substance P (SP), Piezo1, and Piezo2 were determined via immunofluorescence; inflammatory cytokines and neurotransmitters by ELISA; expression of MAPK, Piezo1, and Piezo2 by qRT‐PCR. MAPK expressions were also detected by Western blot.

Results: CCD reduced PWT from day 3, whereas MT significantly restored PWT from day 11. In skin, CCD increased mast cell activation, CGRP, SP, tumor necrosis factor (TNF)‐α, interleukin (IL)‐6, IL‐1β, 5‐hydroxytryptamine (5‐HT), and histamine (HA), all of which were attenuated by both interventions. In DRG, CCD elevated p‐ERK, p‐JNK, and p‐p38 MAPK without altering total MAPK expression, and both interventions suppressed MAPK phosphorylation. Piezo2 was upregulated in CCD and reduced by MT and MCI in both tissues. In skin, Piezo1 remained unchanged, while in DRG, CCD downregulated Piezo1 significantly.

Conclusion: MT can relieve CCD‐induced mechanical allodynia, an effect associated with inhibited cutaneous mast cell activation, attenuated peripheral neuroinflammation, and reduced MAPK hyperphosphorylation in the DRG.

Related

  1. Clonal expansion of CAR-T cells harboring lentivector integration in the PLAAT4 gene following anti-CD19 CAR T-cell therapy in a context of cutaneous T-cell lymphoma
    Vincent Guiraud, Clotilde Bravetti, Amélie Guihot +13
    HaematologicaPeer-reviewedCC BY-NC 4.0Full textImmunologyOncologyHAL
  2. Absence of Viable Tumor Cells in Macroscopically Pigmented Regional Lymph Nodes after Immune Checkpoint Inhibitor Therapy in Duodenal Metastasis of Cutaneous Melanoma: A Case Report.
    Ryota Unno, Yoshihiro Shirai, Yoshimasa Nobeyama +8
    Surgical case reportsPeer-reviewedCC BYFull textOncologyEurope PMCPMC
  3. Additional Effects of Manual Therapy to Exercise on Pain and Function in Individuals With Knee Osteoarthritis: A Systematic Review and Meta‐Analysis
    Viviane R. da Silva, Rebecca B. Ramalho, Marcos de Noronha +3
    European journal of pain (London, England)Peer-reviewedCC BYFull textGeneral medicineEurope PMC
  4. Persistent and transient senescent cells contribute to brain-barrier development
    Lauren Ashley Watson, Zoe Adelsheim, Mackenzie J. Carter +9
    CellPeer-reviewedCC BY5 citedNeurologyOpenAlex
  5. The Use of Causal Language in Case Reports and Case Series Reporting Ischemic Stroke Following Cervical Manual Therapy: A Qualitative Study Protocol
    N. Bakaa, M. Atkinson-Graham, J. Plener +17
    International Journal of Qualitative MethodsPeer-reviewedCC BY-NCGeneral biologyEpidemiology & public healthHAL

Cite

BibTeXRIS
BibTeX
@article{yu2026cutaneous,
  title = {{Cutaneous Mast Cells Contribute to the Analgesic Effect of Manual Therapy on CCD Rats}},
  author = {Jiayang Yu and Pingping Sun and Dong Xie and Xinyu Zeng and Xili Chang and Min Fang and Wuquan Sun and Qingguang Zhu and Chongjie Yao},
  journal = {Pain Research \& Management},
  year = {2026},
  month = oct,
  doi = {10.1155/prm/2749795},
  url = {https://doi.org/10.1155/prm/2749795},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Cutaneous Mast Cells Contribute to the Analgesic Effect of Manual Therapy on CCD Rats
AU  - Jiayang Yu
AU  - Pingping Sun
AU  - Dong Xie
AU  - Xinyu Zeng
AU  - Xili Chang
AU  - Min Fang
AU  - Wuquan Sun
AU  - Qingguang Zhu
AU  - Chongjie Yao
PY  - 2026
DA  - 2026/10/26
JO  - Pain Research & Management
DO  - 10.1155/prm/2749795
UR  - https://doi.org/10.1155/prm/2749795
AB  - Background: Lumbar disc herniation (LDH) commonly induces low back and leg pain. Manual therapy (MT) is effective in relieving such pain clinically, but its mechanism remains unclear. Given that acupoint regions are enriched with mast cells that respond to mechanical stimulation, this study explored whether MT alleviates CCD‐evoked neuropathic pain and its association with the modulation of cutaneous mast cells.  Methods: Sprague‐Dawley (SD) rats were randomized into sham, chronic compression of dorsal root ganglion (CCD), MT, and mast cell inhibition (MCI) groups. After CCD modeling, MT was administered at BL57 starting on day 4 for 14 days, while MCI received acupoint cromolyn sodium injection. Paw withdrawal threshold (PWT) was measured. Mast cell activation was assessed using immunofluorescence and toluidine blue staining. Calcitonin gene‐related peptide (CGRP), substance P (SP), Piezo1, and Piezo2 were determined via immunofluorescence; inflammatory cytokines and neurotransmitters by ELISA; expression of MAPK, Piezo1, and Piezo2 by qRT‐PCR. MAPK expressions were also detected by Western blot.  Results: CCD reduced PWT from day 3, whereas MT significantly restored PWT from day 11. In skin, CCD increased mast cell activation, CGRP, SP, tumor necrosis factor (TNF)‐α, interleukin (IL)‐6, IL‐1β, 5‐hydroxytryptamine (5‐HT), and histamine (HA), all of which were attenuated by both interventions. In DRG, CCD elevated p‐ERK, p‐JNK, and p‐p38 MAPK without altering total MAPK expression, and both interventions suppressed MAPK phosphorylation. Piezo2 was upregulated in CCD and reduced by MT and MCI in both tissues. In skin, Piezo1 remained unchanged, while in DRG, CCD downregulated Piezo1 significantly.  Conclusion: MT can relieve CCD‐induced mechanical allodynia, an effect associated with inhibited cutaneous mast cell activation, attenuated peripheral neuroinflammation, and reduced MAPK hyperphosphorylation in the DRG.
SN  - 1203-6765
SN  - 1918-1523
ER  -