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Peer-reviewedCC BY 4.0Journal of Cell Science

Dynamic micropatterning uncovers spatial dynamics of B cell immune synapse formation

  • Blanca Tejeda-González1,2,3
  • Sara Hernández-Pérez1,2,3
  • Elmeri Kiviluoto1,2,3
  • Johanna Ivaska2,3,4
  • Pieta K. Mattila1,2,3
4 affiliations
  1. 1Institute of Biomedicine and MediCity Research Laboratories, University of Turku, 20014 Turku, Finland
  2. 2InFLAMES Research Flagship, University of Turku, 20014 Turku, Finland
  3. 3Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland
  4. 4Department of Life Technologies, University of Turku, 20014 Turku, Finland
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Abstract

In the process of mounting humoral immune responses, B cells typically engage antigens on the surface of antigen-presenting cells at a site termed the immunological synapse (IS). To study this important cell interaction structure, we developed a dynamic micropatterning technique that models IS formation with precise spatial and temporal control. This method enables imaging of B cells before and after B cell antigen receptor (BCR) engagement, in fixed and live samples. We analyzed BCR-proximal signaling in A20 D1.3 mouse B cells and found distinct spatial distribution preferences for different signaling proteins. Also, the size and shape of the initial non-activatory adhesion modulated the signaling outcome. We visualized the formation of the IS in living cells using enhanced-resolution microscopy in three dimensions. We identified different cell behaviors during this process, including the repurposing of pre-existing actin-based protrusions as ready-made building blocks for the IS. Overall, dynamic micropatterning provides powerful insights into the rapid, early steps of IS formation with remarkable spatial and temporal resolution.

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Cite

BibTeXRIS
BibTeX
@article{tejedagonzalez2026dynamic,
  title = {{Dynamic micropatterning uncovers spatial dynamics of B cell immune synapse formation}},
  author = {Blanca Tejeda-González and Sara Hernández-Pérez and Elmeri Kiviluoto and Johanna Ivaska and Pieta K. Mattila},
  journal = {Journal of Cell Science},
  year = {2026},
  month = oct,
  doi = {10.1242/jcs.264876},
  url = {https://doi.org/10.1242/jcs.264876},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Dynamic micropatterning uncovers spatial dynamics of B cell immune synapse formation
AU  - Blanca Tejeda-González
AU  - Sara Hernández-Pérez
AU  - Elmeri Kiviluoto
AU  - Johanna Ivaska
AU  - Pieta K. Mattila
PY  - 2026
DA  - 2026/10/22
JO  - Journal of Cell Science
DO  - 10.1242/jcs.264876
UR  - https://doi.org/10.1242/jcs.264876
AB  - In the process of mounting humoral immune responses, B cells typically engage antigens on the surface of antigen-presenting cells at a site termed the immunological synapse (IS). To study this important cell interaction structure, we developed a dynamic micropatterning technique that models IS formation with precise spatial and temporal control. This method enables imaging of B cells before and after B cell antigen receptor (BCR) engagement, in fixed and live samples. We analyzed BCR-proximal signaling in A20 D1.3 mouse B cells and found distinct spatial distribution preferences for different signaling proteins. Also, the size and shape of the initial non-activatory adhesion modulated the signaling outcome. We visualized the formation of the IS in living cells using enhanced-resolution microscopy in three dimensions. We identified different cell behaviors during this process, including the repurposing of pre-existing actin-based protrusions as ready-made building blocks for the IS. Overall, dynamic micropatterning provides powerful insights into the rapid, early steps of IS formation with remarkable spatial and temporal resolution.
SN  - 0021-9533
SN  - 1477-9137
ER  -