The Proteome of Plasma Extracellular Vesicles in Latent and Active Tuberculosis Reveals Phase‐Specific Composition and Immunometabolic Insights
4 affiliations
- 1Instituto Politécnico Nacional (IPN), Escuela Nacional de Ciencias Biológicas (ENCB), Posgrado en Inmunología
- 2Instituto Politécnico Nacional (IPN), Escuela Nacional de Ciencias Biológicas (ENCB)
- 3Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV‐IPN), Laboratorios Nacionales de Servicios Experimentales (LANSE)
- 4Universidad Nacional Autónoma de México (UNAM), Facultad de Medicina
Abstract
Tuberculosis (TB) is an infectious disease that affects millions of people worldwide. Extracellular vesicles (EVs) are mediators of intercellular communication that participate in the immunopathogenesis of TB. Here, we characterized plasma EVs from healthy individuals and from individuals with latent (LTB) or active TB (ATB). We found increased total protein concentrations in EVs from LTB and ATB individuals compared to healthy controls, without significant differences in EV concentrations, but reduced exosome abundance in LTB. Proteomic and bioinformatic analysis showed that LTB EVs were enriched in proteins associated with the regulation of inflammation, tissue remodeling, and lipid metabolism, including TGFβ and apolipoproteins (APO) A1 and E. In contrast, ATB EVs were enriched in mycobacterial antigens and host proteins associated with acute inflammation, including serum amyloid A1 and A2 (SAA1/A2) and the calgranulin complex proteins S100A8 and S100A9. These host proteins were central and/or cohesive nodes in the protein–protein interaction networks of these EVs. Dot blot analysis confirmed the increased levels of these EV proteins in a second group of individuals with LTB or ATB. The EV levels of mycobacterial antigens correlated positively with the EV levels of SAA1/A2 and S100A8/A9, but negatively with their levels of TGFβ and APOA1. Principal component analysis and hierarchical clustering suggested that the simultaneous analysis of mycobacterial antigens and these EV proteins could discriminate between healthy, LTB, and ATB individuals. Our findings confirm that plasma EVs provide insights into the host immunometabolic response to TB and may be a source of clinically useful biomarkers.
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BibTeX
@article{speregrino2026proteome,
title = {{The Proteome of Plasma Extracellular Vesicles in Latent and Active Tuberculosis Reveals Phase‐Specific Composition and Immunometabolic Insights}},
author = {Eliud S.‐Peregrino and Jessica Castañeda‐Casimiro and Luis Vallejo‐Castillo and Mishelle Soria‐Fragoso and Gloria M. Ortega‐Díaz and Karen I. Mondragón‐Lara and Alfonso Méndez‐Tenorio and Rommel Chacón‐Salinas and Blanca E. García‐Pérez and Jeanet Serafín‐López and Carlos Wong‐Baeza and Víctor Hugo Rosales‐García and Alejandro Hernández‐Solis and Isabel Wong‐Baeza},
journal = {FASEB bioAdvances},
year = {2026},
month = oct,
doi = {10.1096/fba.2026-00238},
url = {https://europepmc.org/article/PMC/PMC13656203},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - The Proteome of Plasma Extracellular Vesicles in Latent and Active Tuberculosis Reveals Phase‐Specific Composition and Immunometabolic Insights
AU - Eliud S.‐Peregrino
AU - Jessica Castañeda‐Casimiro
AU - Luis Vallejo‐Castillo
AU - Mishelle Soria‐Fragoso
AU - Gloria M. Ortega‐Díaz
AU - Karen I. Mondragón‐Lara
AU - Alfonso Méndez‐Tenorio
AU - Rommel Chacón‐Salinas
AU - Blanca E. García‐Pérez
AU - Jeanet Serafín‐López
AU - Carlos Wong‐Baeza
AU - Víctor Hugo Rosales‐García
AU - Alejandro Hernández‐Solis
AU - Isabel Wong‐Baeza
PY - 2026
DA - 2026/10/01
JO - FASEB bioAdvances
DO - 10.1096/fba.2026-00238
UR - https://europepmc.org/article/PMC/PMC13656203
AB - Tuberculosis (TB) is an infectious disease that affects millions of people worldwide. Extracellular vesicles (EVs) are mediators of intercellular communication that participate in the immunopathogenesis of TB. Here, we characterized plasma EVs from healthy individuals and from individuals with latent (LTB) or active TB (ATB). We found increased total protein concentrations in EVs from LTB and ATB individuals compared to healthy controls, without significant differences in EV concentrations, but reduced exosome abundance in LTB. Proteomic and bioinformatic analysis showed that LTB EVs were enriched in proteins associated with the regulation of inflammation, tissue remodeling, and lipid metabolism, including TGFβ and apolipoproteins (APO) A1 and E. In contrast, ATB EVs were enriched in mycobacterial antigens and host proteins associated with acute inflammation, including serum amyloid A1 and A2 (SAA1/A2) and the calgranulin complex proteins S100A8 and S100A9. These host proteins were central and/or cohesive nodes in the protein–protein interaction networks of these EVs. Dot blot analysis confirmed the increased levels of these EV proteins in a second group of individuals with LTB or ATB. The EV levels of mycobacterial antigens correlated positively with the EV levels of SAA1/A2 and S100A8/A9, but negatively with their levels of TGFβ and APOA1. Principal component analysis and hierarchical clustering suggested that the simultaneous analysis of mycobacterial antigens and these EV proteins could discriminate between healthy, LTB, and ATB individuals. Our findings confirm that plasma EVs provide insights into the host immunometabolic response to TB and may be a source of clinically useful biomarkers.
SN - 2573-9832
ER -