The diagnostic value of miR-4281 for knee osteoarthritis and its mechanism of action on the extracellular matrix of chondrocytes and apoptosis.
6 affiliations
- 1Department of Orthopedics, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, China.
- 2Department of Orthopedics, West China Hospital, Sichuan University, Sichuan, 610041, China.
- 3West China School of Nursing, Sichuan, 610041, China.
- 4Department of Comprehensive Orthopedics, Affiliated Hospital of Hebei University, Hebei, 071000, China.
- 5Department of Day Treatment Center, The Third Affiliated Hospital of Naval Medical University, PLA, Shanghai, 201185, China.
- 6Changzhou Wujin People's Hospital, No.2 Yongning North Road, Tianning District, Changzhou, 213000, China. [email protected].
Abstract
Background
The destruction of tissues such as bone and subchondral bone is an important pathological feature of knee osteoarthritis (KOA).Aim
To explore the diagnostic value of serum miR-4281 and its mechanisms influencing KOA.Materials and methods
The mRNA levels of miR-4281, SOX9, COL2A1, MMP3, MMP13 and BCL3 were detected by RT-qPCR. Pearson correlation was used to assess the correlation between serum miR-4281 and WOMAC score or BCL3. The receiver operating characteristic (ROC) curve was used to analyse the diagnostic efficacy of miR-4281 for KOA. Multivariate Logistic regression was used to assess the impact of miR-4281 on KOA. The dual luciferase reporter gene assay demonstrated the relationship between miR-4281 and BCL3. Annexin V-FITC/PI Apoptosis Kit was used to assess the apoptosis level of C28/I2 cells.Results
In KOA patients, serum miR-4281 was significantly upregulated, while BCL3 was markedly downregulated. miR-4281 exhibited a positive correlation with the WOMAC score and demonstrated high diagnostic efficacy for KOA. 10 ng/mL IL-1β induced the expression of miR-4281, MMP3, and MMP13 in C28/I2 cells, as well as cell apoptosis, while inhibiting the expression of SOX9, COL2A1 and BCL3. miR-4281 inhibitors promote SOX9 and COL2A1 expression while suppressing MMP3 and MMP13 expression as well as apoptosis. Silencing BCL3 reversed the mitigating effects of miR-4281 inhibitors on IL-1β-induced extracellular matrix (ECM) degradation and apoptosis in C28/I2 cells.Conclusion
miR-4281 has a good diagnostic efficacy for KOA and it alleviates the ECM degradation and apoptosis of chondrocytes induced by IL-1β by targeting BCL3.
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BibTeX
@article{song2026diagnostic,
title = {{The diagnostic value of miR-4281 for knee osteoarthritis and its mechanism of action on the extracellular matrix of chondrocytes and apoptosis.}},
author = {Wei Song and Qin Yu and Baohui Zhao and Yu Zhang and Zhigang Kong},
journal = {Hereditas},
year = {2026},
month = jul,
doi = {10.1186/s41065-026-00711-9},
url = {https://europepmc.org/article/MED/42919424},
note = {CC BY},
}
RIS
TY - JOUR
TI - The diagnostic value of miR-4281 for knee osteoarthritis and its mechanism of action on the extracellular matrix of chondrocytes and apoptosis.
AU - Wei Song
AU - Qin Yu
AU - Baohui Zhao
AU - Yu Zhang
AU - Zhigang Kong
PY - 2026
DA - 2026/07/01
JO - Hereditas
DO - 10.1186/s41065-026-00711-9
UR - https://europepmc.org/article/MED/42919424
AB - Background The destruction of tissues such as bone and subchondral bone is an important pathological feature of knee osteoarthritis (KOA).Aim To explore the diagnostic value of serum miR-4281 and its mechanisms influencing KOA.Materials and methods The mRNA levels of miR-4281, SOX9, COL2A1, MMP3, MMP13 and BCL3 were detected by RT-qPCR. Pearson correlation was used to assess the correlation between serum miR-4281 and WOMAC score or BCL3. The receiver operating characteristic (ROC) curve was used to analyse the diagnostic efficacy of miR-4281 for KOA. Multivariate Logistic regression was used to assess the impact of miR-4281 on KOA. The dual luciferase reporter gene assay demonstrated the relationship between miR-4281 and BCL3. Annexin V-FITC/PI Apoptosis Kit was used to assess the apoptosis level of C28/I2 cells.Results In KOA patients, serum miR-4281 was significantly upregulated, while BCL3 was markedly downregulated. miR-4281 exhibited a positive correlation with the WOMAC score and demonstrated high diagnostic efficacy for KOA. 10 ng/mL IL-1β induced the expression of miR-4281, MMP3, and MMP13 in C28/I2 cells, as well as cell apoptosis, while inhibiting the expression of SOX9, COL2A1 and BCL3. miR-4281 inhibitors promote SOX9 and COL2A1 expression while suppressing MMP3 and MMP13 expression as well as apoptosis. Silencing BCL3 reversed the mitigating effects of miR-4281 inhibitors on IL-1β-induced extracellular matrix (ECM) degradation and apoptosis in C28/I2 cells.Conclusion miR-4281 has a good diagnostic efficacy for KOA and it alleviates the ECM degradation and apoptosis of chondrocytes induced by IL-1β by targeting BCL3.
SN - 0018-0661
SN - 1601-5223
ER -