Time-Series Transcriptome Reveals Differential Regulation of Flavonoid Biosynthesis in Dendrobium huoshanense Under Musical-Acoustic Stimuli: Insights for Green Horticultural Production.
- 1Department of Arts, Traditional Chinese Medicine Institute of Anhui Dabie Mountain, West Anhui University, Lu'an 237061, China.
Abstract
Background
Sound vibration is an emerging physical signal that influences plant growth and secondary metabolism; however, the genome-wide transcriptional responses of valuable medicinal orchids to complex acoustic stimuli remain largely uncharacterized.Methods
Here, we performed time-series RNA-seq on acclimatized plantlets of Dendrobium huoshanense exposed to two musically structured acoustic stimuli with contrasting acoustic characteristics (Forest Fantasy and Erquan Yingyue) plus a no-music control; leaf samples were collected at 1, 6, 12, and 24 h, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the transcriptome data.Results
The two acoustic treatments triggered distinct transcriptional reprogramming of flavonoid biosynthesis. Early biosynthetic genes chalcone synthase (CHS) and naringenin 7-O-methyltransferase (NOMT) were rapidly up-regulated under Forest Fantasy, whereas late-step modification genes (naringenin 3-dioxygenase (F3H), flavonoid 3'-monooxygenase (F3'H), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), caffeoyl-CoA O-methyltransferase (CCoAOMT)) were persistently suppressed by Erquan Yingyue. Differentially expressed gene (DEG)-derived hub structural genes (cinnamate 4-hydroxylase (C4H), F3'H, CCoAOMT) and co-expressed transcription factor candidates (MYB, WRKY, NAC) were identified via Pearson correlation-based network analysis.Conclusions
These results indicate that D. huoshanense can differentiate complex musical signals at the transcript level, suggesting short-term natural-style acoustic stimulation as a promising potential horticultural strategy for quality improvement of this precious medicinal orchid.
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BibTeX
@article{huang2026timeseries,
title = {{Time-Series Transcriptome Reveals Differential Regulation of Flavonoid Biosynthesis in Dendrobium huoshanense Under Musical-Acoustic Stimuli: Insights for Green Horticultural Production.}},
author = {Mei Huang and Yinhua Liu and Mengru Liu and Yuxiao Ling and Yeyu Yin and Tingting Wang and Wei Wang and Shanyong Yi},
journal = {Genes},
year = {2026},
month = sep,
doi = {10.3390/genes17101178},
url = {https://europepmc.org/article/MED/42938294},
note = {CC BY},
}
RIS
TY - JOUR
TI - Time-Series Transcriptome Reveals Differential Regulation of Flavonoid Biosynthesis in Dendrobium huoshanense Under Musical-Acoustic Stimuli: Insights for Green Horticultural Production.
AU - Mei Huang
AU - Yinhua Liu
AU - Mengru Liu
AU - Yuxiao Ling
AU - Yeyu Yin
AU - Tingting Wang
AU - Wei Wang
AU - Shanyong Yi
PY - 2026
DA - 2026/09/24
JO - Genes
DO - 10.3390/genes17101178
UR - https://europepmc.org/article/MED/42938294
AB - Background Sound vibration is an emerging physical signal that influences plant growth and secondary metabolism; however, the genome-wide transcriptional responses of valuable medicinal orchids to complex acoustic stimuli remain largely uncharacterized.Methods Here, we performed time-series RNA-seq on acclimatized plantlets of Dendrobium huoshanense exposed to two musically structured acoustic stimuli with contrasting acoustic characteristics (Forest Fantasy and Erquan Yingyue) plus a no-music control; leaf samples were collected at 1, 6, 12, and 24 h, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the transcriptome data.Results The two acoustic treatments triggered distinct transcriptional reprogramming of flavonoid biosynthesis. Early biosynthetic genes chalcone synthase (CHS) and naringenin 7-O-methyltransferase (NOMT) were rapidly up-regulated under Forest Fantasy, whereas late-step modification genes (naringenin 3-dioxygenase (F3H), flavonoid 3'-monooxygenase (F3'H), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), caffeoyl-CoA O-methyltransferase (CCoAOMT)) were persistently suppressed by Erquan Yingyue. Differentially expressed gene (DEG)-derived hub structural genes (cinnamate 4-hydroxylase (C4H), F3'H, CCoAOMT) and co-expressed transcription factor candidates (MYB, WRKY, NAC) were identified via Pearson correlation-based network analysis.Conclusions These results indicate that D. huoshanense can differentiate complex musical signals at the transcript level, suggesting short-term natural-style acoustic stimulation as a promising potential horticultural strategy for quality improvement of this precious medicinal orchid.
SN - 2073-4425
ER -