The Pupil‐Brain System at Rest: Spontaneous Pupil Fluctuations as Markers of Neuromodulatory and Network Dynamics
- 1Department of Neuropsychology & Psychopharmacology, Faculty of Psychology and Neuroscience Maastricht University Maastricht the Netherlands
- 2Department of Neuropsychology Max Planck Institute for Human Cognitive and Brain Sciences Leipzig Germany
Abstract
Spontaneous pupil fluctuations (SPFs) during rest provide a non-invasive, low-cost index of central arousal dynamics, independent of cognitive task demands. These features position SPFs as promising markers for baseline neurophysiological activity in both basic and translational research. This review synthesizes current evidence on the resting-state pupil-brain system, focusing on its core components: central neuromodulatory circuits and large-scale cortical networks. We first examine the relationship between SPFs and distributed elements of the ascending arousal system, including noradrenergic, cholinergic, serotonergic, and dopaminergic nuclei. We then detail SPF coupling with intrinsic functional networks-default mode, salience, and sensorimotor systems-highlighting their role in mediating transitions between internally- and externally-oriented brain states. Finally, we discuss the spectral and directional properties of pupil-brain interactions, emphasizing analytical approaches suited for assessing directionality and outlining implications for clinical and translational applications. Converging evidence from animal and human neuroimaging studies reveals robust spatiotemporal and spectral coupling between SPFs and neural activity across micro- and macro-scales. These findings support a systems-level framework in which SPFs serve as integrative markers linking subcortical neuromodulation with large-scale cortical dynamics. We conclude that SPFs offer a sensitive window into arousal regulation and body-brain integration, with potential utility as biomarkers for neuropsychiatric conditions and altered states of consciousness.
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BibTeX
@article{liu2026pupilbrain,
title = {{The Pupil‐Brain System at Rest: Spontaneous Pupil Fluctuations as Markers of Neuromodulatory and Network Dynamics}},
author = {Tongxin Liu and Sonja A. Kotz and Antonio Criscuolo and Michael Schwartze},
journal = {Psychophysiology},
year = {2026},
month = jun,
doi = {10.1111/psyp.70338},
url = {https://doi.org/10.1111/psyp.70338},
note = {CC BY},
}
RIS
TY - JOUR
TI - The Pupil‐Brain System at Rest: Spontaneous Pupil Fluctuations as Markers of Neuromodulatory and Network Dynamics
AU - Tongxin Liu
AU - Sonja A. Kotz
AU - Antonio Criscuolo
AU - Michael Schwartze
PY - 2026
DA - 2026/06/01
JO - Psychophysiology
DO - 10.1111/psyp.70338
UR - https://doi.org/10.1111/psyp.70338
AB - Spontaneous pupil fluctuations (SPFs) during rest provide a non-invasive, low-cost index of central arousal dynamics, independent of cognitive task demands. These features position SPFs as promising markers for baseline neurophysiological activity in both basic and translational research. This review synthesizes current evidence on the resting-state pupil-brain system, focusing on its core components: central neuromodulatory circuits and large-scale cortical networks. We first examine the relationship between SPFs and distributed elements of the ascending arousal system, including noradrenergic, cholinergic, serotonergic, and dopaminergic nuclei. We then detail SPF coupling with intrinsic functional networks-default mode, salience, and sensorimotor systems-highlighting their role in mediating transitions between internally- and externally-oriented brain states. Finally, we discuss the spectral and directional properties of pupil-brain interactions, emphasizing analytical approaches suited for assessing directionality and outlining implications for clinical and translational applications. Converging evidence from animal and human neuroimaging studies reveals robust spatiotemporal and spectral coupling between SPFs and neural activity across micro- and macro-scales. These findings support a systems-level framework in which SPFs serve as integrative markers linking subcortical neuromodulation with large-scale cortical dynamics. We conclude that SPFs offer a sensitive window into arousal regulation and body-brain integration, with potential utility as biomarkers for neuropsychiatric conditions and altered states of consciousness.
SN - 0048-5772
SN - 1469-8986
ER -