Invasive and Non-invasive Biomarkers to Study the Genetic Instability among Polycystic Ovarian Syndrome Women of South Indian Cohort.
4 affiliations
- 1Department of Computer and Bioscience, Shri Vaishnav Institute of Management and Science, Indore, Madhya Pradesh, India.
- 2Department of Biomedical Sciences, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
- 3Department of Biochemistry, School of Life Sciences (Ooty Campus), JSS Academy of Higher Education and Research (Mysuru), Ooty, Tamil Nadu, India.
- 4Department of Biotechnology, School of Life Sciences (Ooty Campus), JSS Academy of Higher Education and Research (Mysuru), Ooty, Tamil Nadu, India.
Abstract
Background
Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with reproductive and metabolic abnormalities. Emerging evidence suggests that genomic instability may contribute to its pathophysiology and long-term health consequences.Aim
The aim of this study was to evaluate genomic instability in women with PCOS using invasive and non-invasive cytogenetic biomarkers.Settings and design
A case-control study was conducted among women with PCOS and age-matched healthy controls from a South Indian cohort.Materials and methods
Buccal epithelial cells and peripheral blood samples were collected from 200 women with PCOS and 100 healthy controls. Genomic instability was assessed using the buccal micronucleus cytome (BMCyt) assay, cytokinesis-block micronucleus (CBMN) assay and chromosomal aberration analysis.Statistical analysis used
Data were analysed using GraphPad Prism 6.0. Group comparisons were performed using Student's unpaired t-test or Mann-Whitney U test as appropriate, with P < 0.05 considered statistically significant.Results
Women with PCOS exhibited increased frequencies of nucleoplasmic bridges, nuclear buds, micronucleated binucleated cells and chromosomal aberrations compared with controls. Higher frequencies of chromosomal breaks, chromatid breaks and dicentric chromosomes were also observed, indicating increased genomic instability in the PCOS group.Conclusion
Women with PCOS demonstrated increased cytogenetic abnormalities suggestive of elevated genomic instability. The combined application of BMCyt, CBMN and chromosomal aberration analyses provides complementary information regarding deoxyribonucleic acid damage and chromosomal instability in PCOS and may be useful for genomic instability assessment in future studies.
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BibTeX
@article{mukherjee2026invasive,
title = {{Invasive and Non-invasive Biomarkers to Study the Genetic Instability among Polycystic Ovarian Syndrome Women of South Indian Cohort.}},
author = {Anirban Goutam Mukherjee and V G Abilash and L Hariprasath and S P Tamilarasi and S Nishu},
journal = {Journal of human reproductive sciences},
year = {2026},
month = jul,
doi = {10.4103/jhrs.jhrs_225_26},
url = {https://europepmc.org/article/MED/42923889},
note = {CC BY-NC-SA},
}
RIS
TY - JOUR
TI - Invasive and Non-invasive Biomarkers to Study the Genetic Instability among Polycystic Ovarian Syndrome Women of South Indian Cohort.
AU - Anirban Goutam Mukherjee
AU - V G Abilash
AU - L Hariprasath
AU - S P Tamilarasi
AU - S Nishu
PY - 2026
DA - 2026/07/01
JO - Journal of human reproductive sciences
DO - 10.4103/jhrs.jhrs_225_26
UR - https://europepmc.org/article/MED/42923889
AB - Background Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with reproductive and metabolic abnormalities. Emerging evidence suggests that genomic instability may contribute to its pathophysiology and long-term health consequences.Aim The aim of this study was to evaluate genomic instability in women with PCOS using invasive and non-invasive cytogenetic biomarkers.Settings and design A case-control study was conducted among women with PCOS and age-matched healthy controls from a South Indian cohort.Materials and methods Buccal epithelial cells and peripheral blood samples were collected from 200 women with PCOS and 100 healthy controls. Genomic instability was assessed using the buccal micronucleus cytome (BMCyt) assay, cytokinesis-block micronucleus (CBMN) assay and chromosomal aberration analysis.Statistical analysis used Data were analysed using GraphPad Prism 6.0. Group comparisons were performed using Student's unpaired t-test or Mann-Whitney U test as appropriate, with P < 0.05 considered statistically significant.Results Women with PCOS exhibited increased frequencies of nucleoplasmic bridges, nuclear buds, micronucleated binucleated cells and chromosomal aberrations compared with controls. Higher frequencies of chromosomal breaks, chromatid breaks and dicentric chromosomes were also observed, indicating increased genomic instability in the PCOS group.Conclusion Women with PCOS demonstrated increased cytogenetic abnormalities suggestive of elevated genomic instability. The combined application of BMCyt, CBMN and chromosomal aberration analyses provides complementary information regarding deoxyribonucleic acid damage and chromosomal instability in PCOS and may be useful for genomic instability assessment in future studies.
SN - 0974-1208
SN - 1998-4766
ER -