Circular RNA circ_0006523 promotes lipid accumulation and proliferation of diffuse large B-cell lymphoma via the miR-659-3p/SPHK1 axis
Abstract
Dysregulated lipid metabolism is a core hallmark of diffuse large B-cell lymphoma (DLBCL), while the upstream non-coding RNA regulatory network targeting sphingosine kinase 1 (SPHK1), a key lipogenic kinase, remains largely uncharacterized in DLBCL. Here, we identified and validated a novel ceRNA regulatory axis consisting of circ_0006523, miR-659-3p, and SPHK1 in DLBCL clinical tissues and cell lines. circ_0006523 and SPHK1 were significantly upregulated, whereas miR-659-3p was downregulated, in DLBCL tissues and cell lines; high circ_0006523 expression was associated with advanced Ann Arbor stages (Ⅲ–IV). Mechanistically, RNase R-resistant circ_0006523 acted as a competing endogenous RNA (ceRNA) to sponge miR-659-3p, thus relieving its repression on SPHK1. Functionally, circ_0006523 promoted DLBCL cell proliferation and elevated intracellular lipid abundance via the miR-659-3p/SPHK1 axis. Collectively, the circ_0006523/miR-659-3p/SPHK1 axis modulates DLBCL proliferative phenotypes in vitro. Circ_0006523 expression correlates with advanced Ann Arbor stage in a limited DLBCL cohort, and this axis may provide preliminary molecular insights for further investigation in DLBCL.
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BibTeX
@article{zhou2026circular,
title = {{Circular RNA circ_0006523 promotes lipid accumulation and proliferation of diffuse large B-cell lymphoma via the miR-659-3p/SPHK1 axis}},
author = {Qixiang Zhou and Yubo Yang and Xingyan Guo and Cuijuan Qian and Jun Yao},
journal = {Scientific Reports},
year = {2026},
month = oct,
doi = {10.1038/s41598-026-73888-8},
url = {https://www.nature.com/articles/s41598-026-73888-8},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Circular RNA circ_0006523 promotes lipid accumulation and proliferation of diffuse large B-cell lymphoma via the miR-659-3p/SPHK1 axis
AU - Qixiang Zhou
AU - Yubo Yang
AU - Xingyan Guo
AU - Cuijuan Qian
AU - Jun Yao
PY - 2026
DA - 2026/10/07
JO - Scientific Reports
DO - 10.1038/s41598-026-73888-8
UR - https://www.nature.com/articles/s41598-026-73888-8
AB - Dysregulated lipid metabolism is a core hallmark of diffuse large B-cell lymphoma (DLBCL), while the upstream non-coding RNA regulatory network targeting sphingosine kinase 1 (SPHK1), a key lipogenic kinase, remains largely uncharacterized in DLBCL. Here, we identified and validated a novel ceRNA regulatory axis consisting of circ_0006523, miR-659-3p, and SPHK1 in DLBCL clinical tissues and cell lines. circ_0006523 and SPHK1 were significantly upregulated, whereas miR-659-3p was downregulated, in DLBCL tissues and cell lines; high circ_0006523 expression was associated with advanced Ann Arbor stages (Ⅲ–IV). Mechanistically, RNase R-resistant circ_0006523 acted as a competing endogenous RNA (ceRNA) to sponge miR-659-3p, thus relieving its repression on SPHK1. Functionally, circ_0006523 promoted DLBCL cell proliferation and elevated intracellular lipid abundance via the miR-659-3p/SPHK1 axis. Collectively, the circ_0006523/miR-659-3p/SPHK1 axis modulates DLBCL proliferative phenotypes in vitro. Circ_0006523 expression correlates with advanced Ann Arbor stage in a limited DLBCL cohort, and this axis may provide preliminary molecular insights for further investigation in DLBCL.
SN - 2045-2322
ER -