Ameliorative Effect of a Chenopodium formosanum-Derived Peptide on Diabetic Wound Healing and Fibroblast Dysfunction
10 affiliations
- 1Department of Clinical Nutrition, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China
- 2Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan
- 3Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan
- 4Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan
- 5Department of Optometry, Asia University, Taichung 41354, Taiwan
- 6Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan
- 7Graduate Program of Nutrition Science, National Taiwan Normal University, Taipei 11677, Taiwan
- 8School of Food Safety, Taipei Medical University, Taipei 11031, Taiwan
- 9School of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan
- 10Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan
Abstract
Oxidative stress contributes to fibroblast dysfunction in diabetic wounds. We evaluated a previously characterized glycine-rich peptide derived from Rhizopus oligosporus-fermented germinated Chenopodium formosanum (FGP) in male diabetic db/db mice and NIH3T3 fibroblasts. Topical FGP (20 or 100 ppm) was assessed by wound-area measurements and histology over 14 days, whereas fibroblasts exposed to 100 mM glucose were evaluated for viability, gap closure, intracellular reactive oxygen species (ROS), type I collagen expression, and repair-associated proteins. On day 14, wound contraction reached 77.6% with 100 ppm FGP and was greater than in untreated wounds (p < 0.05). In fibroblasts, 50 and 100 ppm FGP reduced ROS by approximately 37% and 79%, increased type I collagen expression by approximately 80–90%, and reduced the p-p65 NF-κB/p65 NF-κB ratio by 38% and 48% and the MMP-9/TIMP-1 ratio by 61% and 66%, respectively (p < 0.05). Both concentrations also improved gap closure, whereas VEGF expression increased significantly only at 50 ppm. Among the concentrations tested, 100 ppm showed the most consistent overall activity and is proposed as the preferred concentration for further preclinical evaluation, although formal dose optimization remains necessary. These findings support further investigation of FGP for diabetic wound repair, while tissue-level mechanisms and long-term outcomes remain to be established.
Related
- Changes of serum fibroblast growth factor 23 (FGF23) and Klotho in diabetic kidney disease and their correlation with coronary artery calcification: a retrospective study.
- Single-cell transcriptomic analysis reveals fibroblast state-specific remodeling of purinergic signaling in diabetic gingiva
- Lactylation-driven mitochondrial dysfunction promotes diabetic myocardial fibrosis via impairing mitophagy and activating TGF-β1
- Distinct Liver Enzyme Profiles in Non-Diabetic Obesity: Associations with Obesity Severity and Metabolic Dysfunction
- Exercise mitigates cardiac dysfunction post MI in ovariectomized mice via miR-21/Sox7-mediated suppression of cardiac fibroblast activation
BibTeX
@article{ko2026ameliorative,
title = {{Ameliorative Effect of a Chenopodium formosanum-Derived Peptide on Diabetic Wound Healing and Fibroblast Dysfunction}},
author = {Chih-Yuan Ko and Kuan-Chen Cheng and Yi-Ling Lin and Shin-Ping Lin and Yi-Chen Chen and Chung-Hsin Wu and Thi Kim Ngan Nguyen and Yi-Ping Chuang and Ting-Hua Lai and Chen-Wen Lu and Chen-Che Hsieh and Szu-Chuan Shen},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27219541},
url = {https://www.mdpi.com/1422-0067/27/21/9541},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Ameliorative Effect of a Chenopodium formosanum-Derived Peptide on Diabetic Wound Healing and Fibroblast Dysfunction
AU - Chih-Yuan Ko
AU - Kuan-Chen Cheng
AU - Yi-Ling Lin
AU - Shin-Ping Lin
AU - Yi-Chen Chen
AU - Chung-Hsin Wu
AU - Thi Kim Ngan Nguyen
AU - Yi-Ping Chuang
AU - Ting-Hua Lai
AU - Chen-Wen Lu
AU - Chen-Che Hsieh
AU - Szu-Chuan Shen
PY - 2026
DA - 2026/10/25
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27219541
UR - https://www.mdpi.com/1422-0067/27/21/9541
AB - Oxidative stress contributes to fibroblast dysfunction in diabetic wounds. We evaluated a previously characterized glycine-rich peptide derived from Rhizopus oligosporus-fermented germinated Chenopodium formosanum (FGP) in male diabetic db/db mice and NIH3T3 fibroblasts. Topical FGP (20 or 100 ppm) was assessed by wound-area measurements and histology over 14 days, whereas fibroblasts exposed to 100 mM glucose were evaluated for viability, gap closure, intracellular reactive oxygen species (ROS), type I collagen expression, and repair-associated proteins. On day 14, wound contraction reached 77.6% with 100 ppm FGP and was greater than in untreated wounds (p < 0.05). In fibroblasts, 50 and 100 ppm FGP reduced ROS by approximately 37% and 79%, increased type I collagen expression by approximately 80–90%, and reduced the p-p65 NF-κB/p65 NF-κB ratio by 38% and 48% and the MMP-9/TIMP-1 ratio by 61% and 66%, respectively (p < 0.05). Both concentrations also improved gap closure, whereas VEGF expression increased significantly only at 50 ppm. Among the concentrations tested, 100 ppm showed the most consistent overall activity and is proposed as the preferred concentration for further preclinical evaluation, although formal dose optimization remains necessary. These findings support further investigation of FGP for diabetic wound repair, while tissue-level mechanisms and long-term outcomes remain to be established.
SN - 1422-0067
ER -