Extracellular vesicle heterogeneity through the lens of multiomics
Abstract
Extracellular vesicles (EVs) are heterogeneous in size, biogenesis, content, and function.Aggressive cancer cells release a distinct, poorly characterized and particularly large EV subtype referred to as large oncosomes (LO). This study employs an optimized method to improve preparatory yields of LO and integrates mass spectrometry and RNAsequencing data to profile their molecular content. A consistent set of proteins enriched in LO is identified across glioma, prostate, and breast cancer cell lines. These species are also largely present as mRNA in LO from the prostate cancer model and abundant in plasma LO from patients with metastasis. Single LO RNA-sequencing confirms the findings from bulk LO cargo, demonstrating the utility of single-cell technologies for large vesicle analysis. These results demonstrate the feasibility of multiomic approaches to characterize EV composition, and further suggest the potential prognostic value of LO for developing a multi-analyte approach for liquid biopsy.
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BibTeX
@article{silva2026extracellular,
title = {{Extracellular vesicle heterogeneity through the lens of multiomics}},
author = {Taylon Silva and Elizabeth Hutchins and Wenyan Zhao and Yari Ciani and Minhyung Kim and Emily Ko and Javier Mariscal and Zhuyu Qiu and Fatima Bedier and Agnes Kittel and Bo Zhou and Yang Wang and Megan Hall and Francesca Galasso and Rebecca Reiman and Michael Freeman and Sarah Parker and Jennifer van Eyk and Wei Yang and Edwin Posadas and Jlenia Guarnerio and John Nolan and Clotilde Théry and Andries Zijlstra and Shannon Stott and Sungyong You and Francesca Demichelis and Paul Boutros and Kendall van Keuren-Jensen and Dolores Di Vizio},
journal = {Cell Reports Medicine},
year = {2026},
month = oct,
doi = {10.1016/j.xcrm.2025.102161},
url = {https://inserm.hal.science/inserm-05779167v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Extracellular vesicle heterogeneity through the lens of multiomics
AU - Taylon Silva
AU - Elizabeth Hutchins
AU - Wenyan Zhao
AU - Yari Ciani
AU - Minhyung Kim
AU - Emily Ko
AU - Javier Mariscal
AU - Zhuyu Qiu
AU - Fatima Bedier
AU - Agnes Kittel
AU - Bo Zhou
AU - Yang Wang
AU - Megan Hall
AU - Francesca Galasso
AU - Rebecca Reiman
AU - Michael Freeman
AU - Sarah Parker
AU - Jennifer van Eyk
AU - Wei Yang
AU - Edwin Posadas
AU - Jlenia Guarnerio
AU - John Nolan
AU - Clotilde Théry
AU - Andries Zijlstra
AU - Shannon Stott
AU - Sungyong You
AU - Francesca Demichelis
AU - Paul Boutros
AU - Kendall van Keuren-Jensen
AU - Dolores Di Vizio
PY - 2026
DA - 2026/10/05
JO - Cell Reports Medicine
DO - 10.1016/j.xcrm.2025.102161
UR - https://inserm.hal.science/inserm-05779167v1
AB - Extracellular vesicles (EVs) are heterogeneous in size, biogenesis, content, and function.Aggressive cancer cells release a distinct, poorly characterized and particularly large EV subtype referred to as large oncosomes (LO). This study employs an optimized method to improve preparatory yields of LO and integrates mass spectrometry and RNAsequencing data to profile their molecular content. A consistent set of proteins enriched in LO is identified across glioma, prostate, and breast cancer cell lines. These species are also largely present as mRNA in LO from the prostate cancer model and abundant in plasma LO from patients with metastasis. Single LO RNA-sequencing confirms the findings from bulk LO cargo, demonstrating the utility of single-cell technologies for large vesicle analysis. These results demonstrate the feasibility of multiomic approaches to characterize EV composition, and further suggest the potential prognostic value of LO for developing a multi-analyte approach for liquid biopsy.
SN - 2666-3791
ER -