Phenolic Profiles and DPPH Radical-Scavenging Activity of Two Eranthis Species from Southeastern Siberia
7 affiliations
- 1Central Siberian Botanical Garden, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk 630090, Russia
- 2Komarov Botanical Institute of Russian Academy of Sciences, St. Petersburg 197022, Russia
- 3Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk 630090, Russia
- 4Department of Natural Sciences, Novosibirsk State University, Novosibirsk 630090, Russia
- 5Faculty of Chemistry, National Research Tomsk State University, Tomsk 634028, Russia
- 6Laboratory of the Herbarium, Tomsk State University, Tomsk 634050, Russia
- 7State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
Abstract
This study focused on Eranthis sibirica and E. tanhoensis (Ranunculaceae), endemic species of Southeastern Siberia. Until recently, these plants had not been sufficiently studied from a phytochemical perspective. The aim of this work was to analyze the phenolic composition and antioxidant properties of 70% ethanolic extracts of their leaves. Liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) was used to analyze metabolites in Eranthis sibirica and E. tanhoensis extracts. The free-radical-scavenging ability of the extracts was assessed using the DPPH method. LC–HRMS metabolomic profiling of the leaf extracts from E. sibirica and E. tanhoensis revealed a diverse range of phenolic constituents; among them, 25 compounds were annotated for the first time for this genus, while the remaining 19 had been previously reported. These included flavonols (16 components), flavanones (8), flavones and isoflavones (5), chalcones (5), flavans (1), catechins (1), and phenolic acids and their derivatives (8). The E. tanhoensis leaf extracts were found to have higher phenolic content (total phenolic content of 3.07 g per 100 g dry weight) and greater DPPH radical scavenging activity (IC50 = 268.45 μg/mL dry weight) than E. sibirica. These findings indicate that the species with the higher phenolic accumulation also displays stronger antioxidant activity, suggesting that both species—particularly E. tanhoensis—warrant further phytochemical and biological investigation.
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BibTeX
@article{kostikova2026phenolic,
title = {{Phenolic Profiles and DPPH Radical-Scavenging Activity of Two Eranthis Species from Southeastern Siberia}},
author = {Vera A. Kostikova and Natalia V. Petrova and Alexander A. Chernonosov and Vladimir V. Koval and Maria V. Lyapunova and Yulia D. Razgulyaeva and Evgeniia R. Lazareva and Alexander A. Kuznetsov and Kun-Li Xiang and Wei Wang and Andrey S. Erst},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = nov,
doi = {10.3390/ijms27219973},
url = {https://www.mdpi.com/1422-0067/27/21/9973},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Phenolic Profiles and DPPH Radical-Scavenging Activity of Two Eranthis Species from Southeastern Siberia
AU - Vera A. Kostikova
AU - Natalia V. Petrova
AU - Alexander A. Chernonosov
AU - Vladimir V. Koval
AU - Maria V. Lyapunova
AU - Yulia D. Razgulyaeva
AU - Evgeniia R. Lazareva
AU - Alexander A. Kuznetsov
AU - Kun-Li Xiang
AU - Wei Wang
AU - Andrey S. Erst
PY - 2026
DA - 2026/11/06
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27219973
UR - https://www.mdpi.com/1422-0067/27/21/9973
AB - This study focused on Eranthis sibirica and E. tanhoensis (Ranunculaceae), endemic species of Southeastern Siberia. Until recently, these plants had not been sufficiently studied from a phytochemical perspective. The aim of this work was to analyze the phenolic composition and antioxidant properties of 70% ethanolic extracts of their leaves. Liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) was used to analyze metabolites in Eranthis sibirica and E. tanhoensis extracts. The free-radical-scavenging ability of the extracts was assessed using the DPPH method. LC–HRMS metabolomic profiling of the leaf extracts from E. sibirica and E. tanhoensis revealed a diverse range of phenolic constituents; among them, 25 compounds were annotated for the first time for this genus, while the remaining 19 had been previously reported. These included flavonols (16 components), flavanones (8), flavones and isoflavones (5), chalcones (5), flavans (1), catechins (1), and phenolic acids and their derivatives (8). The E. tanhoensis leaf extracts were found to have higher phenolic content (total phenolic content of 3.07 g per 100 g dry weight) and greater DPPH radical scavenging activity (IC50 = 268.45 μg/mL dry weight) than E. sibirica. These findings indicate that the species with the higher phenolic accumulation also displays stronger antioxidant activity, suggesting that both species—particularly E. tanhoensis—warrant further phytochemical and biological investigation.
SN - 1422-0067
ER -