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Peer-reviewedCC BY-NC-ND 4.0STAR Protocols

Protocol for transcriptional profiling of fiber type and regeneration markers in mouse Tibialis Anterior muscle

  • Zuleyha Yildirim
  • Aurélia Noll
  • Jean-Christophe Amé
  • Françoise Dantzer
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Abstract

Skeletal muscle integrity and function depend on fiber-type composition and regenerative capacity. Evaluating both components in normal and injured tissues provides valuable insights into subtle declines in muscle function and recovery. We describe a protocol for the transcriptional analysis of the major fast-twitch type II myofibers and regeneration-related genes in normal and local-injured mouse Tibialis Anterior skeletal muscles. We describe steps for integrating freeze-induced injury, sample collection, tissue dissociation, RNA extraction, and gene expression analysis by quantitative reverse-transcription PCR (RT-qPCR).

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Cite

BibTeXRIS
BibTeX
@article{yildirim2026protocol,
  title = {{Protocol for transcriptional profiling of fiber type and regeneration markers in mouse Tibialis Anterior muscle}},
  author = {Zuleyha Yildirim and Aurélia Noll and Jean-Christophe Amé and Françoise Dantzer},
  journal = {STAR Protocols},
  year = {2026},
  month = jul,
  doi = {10.1016/j.xpro.2026.104734},
  url = {https://univoak.hal.science/hal-05750501v1},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - Protocol for transcriptional profiling of fiber type and regeneration markers in mouse Tibialis Anterior muscle
AU  - Zuleyha Yildirim
AU  - Aurélia Noll
AU  - Jean-Christophe Amé
AU  - Françoise Dantzer
PY  - 2026
DA  - 2026/07/29
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2026.104734
UR  - https://univoak.hal.science/hal-05750501v1
AB  - Skeletal muscle integrity and function depend on fiber-type composition and regenerative capacity. Evaluating both components in normal and injured tissues provides valuable insights into subtle declines in muscle function and recovery. We describe a protocol for the transcriptional analysis of the major fast-twitch type II myofibers and regeneration-related genes in normal and local-injured mouse Tibialis Anterior skeletal muscles. We describe steps for integrating freeze-induced injury, sample collection, tissue dissociation, RNA extraction, and gene expression analysis by quantitative reverse-transcription PCR (RT-qPCR).
SN  - 2666-1667
ER  -