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Peer-reviewedCC BY 4.0Journal of Biological Chemistry

Carbonic Anhydrase in the Scleractinian Coral Stylophora pistillata

  • Aurélie Moya
  • Sylvie Tambutté
  • Anthony Bertucci
  • Eric Tambutté
  • Séverine Lotto
  • Daniela Vullo
  • Claudiu T Supuran
  • Denis Allemand
  • Didier Zoccola
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Abstract

Carbonic anhydrases (CA) play an important role in biomineralization from invertebrates to vertebrates. Previous experiments have investigated the role of CA in coral calcification, mainly by pharmacological approaches. This study reports the molecular cloning, sequencing, and immunolocalization of a CA isolated from the scleractinian coral Stylophora pistillata, named STPCA. Results show that STPCA is a secreted form of ␣-CA, which possesses a CA catalytic function, similar to the secreted human CAVI. We localized this enzyme at the calicoblastic ectoderm level, which is responsible for the precipitation of the skeleton. This localization supports the role of STPCA in the calcification process. In symbiotic scleractinian corals, calcification is stimulated by light, a phenomenon called "lightenhanced calcification" (LEC). The mechanism by which symbiont photosynthesis stimulates calcification is still enigmatic. We tested the hypothesis that coral genes are differentially expressed under light and dark conditions. By real-time PCR, we investigated the differential expression of STPCA to determine its role in the LEC phenomenon. Results show that the STPCA gene is expressed 2-fold more during the dark than the light. We suggest that in the dark, up-regulation of the STPCA gene represents a mechanism to cope with night acidosis.

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Cite

BibTeXRIS
BibTeX
@article{moya2026carbonic,
  title = {{Carbonic Anhydrase in the Scleractinian Coral Stylophora pistillata}},
  author = {Aurélie Moya and Sylvie Tambutté and Anthony Bertucci and Eric Tambutté and Séverine Lotto and Daniela Vullo and Claudiu T Supuran and Denis Allemand and Didier Zoccola},
  journal = {Journal of Biological Chemistry},
  year = {2026},
  month = sep,
  doi = {10.1074/jbc.m804726200},
  url = {https://hal.science/hal-05736702v1},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Carbonic Anhydrase in the Scleractinian Coral Stylophora pistillata
AU  - Aurélie Moya
AU  - Sylvie Tambutté
AU  - Anthony Bertucci
AU  - Eric Tambutté
AU  - Séverine Lotto
AU  - Daniela Vullo
AU  - Claudiu T Supuran
AU  - Denis Allemand
AU  - Didier Zoccola
PY  - 2026
DA  - 2026/09/03
JO  - Journal of Biological Chemistry
DO  - 10.1074/jbc.m804726200
UR  - https://hal.science/hal-05736702v1
AB  - Carbonic anhydrases (CA) play an important role in biomineralization from invertebrates to vertebrates. Previous experiments have investigated the role of CA in coral calcification, mainly by pharmacological approaches. This study reports the molecular cloning, sequencing, and immunolocalization of a CA isolated from the scleractinian coral Stylophora pistillata, named STPCA. Results show that STPCA is a secreted form of ␣-CA, which possesses a CA catalytic function, similar to the secreted human CAVI. We localized this enzyme at the calicoblastic ectoderm level, which is responsible for the precipitation of the skeleton. This localization supports the role of STPCA in the calcification process. In symbiotic scleractinian corals, calcification is stimulated by light, a phenomenon called "lightenhanced calcification" (LEC). The mechanism by which symbiont photosynthesis stimulates calcification is still enigmatic. We tested the hypothesis that coral genes are differentially expressed under light and dark conditions. By real-time PCR, we investigated the differential expression of STPCA to determine its role in the LEC phenomenon. Results show that the STPCA gene is expressed 2-fold more during the dark than the light. We suggest that in the dark, up-regulation of the STPCA gene represents a mechanism to cope with night acidosis.
SN  - 0021-9258
SN  - 1083-351X
ER  -