Tonotopically distinct OFF responses arise in the mouse auditory midbrain following sideband suppression
4 affiliations
- 1The Solomon H. Snyder Department of Neuroscience Johns Hopkins University Baltimore Maryland USA
- 2Department of Otolaryngology‐Head and Neck Surgery Johns Hopkins University Baltimore Maryland USA
- 3The Center for Functional Anatomy & Evolution Johns Hopkins University Baltimore Maryland USA
- 4Kavli Neuroscience Discovery Institute Johns Hopkins University Baltimore Maryland USA
Abstract
The parsing of sensory information into discrete topographic domains is a fundamental principle of sensory processing. In the auditory cortex, these domains evolve during a stimulus, with the onset and offset of tones evoking distinct spatial patterns of neural activity. However, it is not known where in the auditory system this spatial segregation occurs or how these dynamics are affected by hearing loss. Using widefield single photon neuronal Ca2+ imaging in the inferior colliculus (IC) of awake mice, we found that pure tone stimuli elicited both spatially constrained neural activity within isofrequency bands and simultaneous sideband suppression. At cessation of the stimulus, offset responses emerged within the region of sideband suppression, demonstrating that simple stimuli elicit spatiotemporally distinct neural activity patterns to represent the presence of sound and sound termination. Because sound frequency is spatially encoded in the IC, this spatial shift creates a tonotopically distinct offset (tdOFF) response relative to sound onset. High‐resolution two‐photon Ca2+ imaging confirmed that tdOFF neuron activity in the sideband region was suppressed during sound and elevated above baseline after stimulus termination, raising the possibility that rebound excitation could contribute to this post‐stimulus activation. Loud noise exposure – a common model of hearing loss – abolished both sideband suppression and tdOFF responses. These results show that hearing loss profoundly reshapes the spatiotemporal pattern of sound processing by altering sideband activity. This preferential loss of sideband suppression and tdOFF activation after sound‐induced injury in the auditory midbrain may contribute to hyperacusis and tinnitus by promoting neuronal hyperactivity.
Key points: Sensory systems encode different features of stimuli by activating distinct neural networks.
Sound onsets and offsets elicit distinct neural patterns in the auditory cortex, although it is unclear where this separation originates or how it may change with hearing loss.
Using in vivo widefield Ca2+ imaging in awake mice, we find that pure tone stimuli evoke spatiotemporally distinct on and off patterns of neural activity in the auditory midbrain.
Neurons active during stimulus offset were suppressed by sound in sideband regions, raising the possibility that rebound excitation may contribute to this post‐stimulus activation.
Both sideband suppression and off responses were preferentially abolished following noise‐induced hearing loss, raising the possibility that these changes may contribute to hearing loss‐related syndromes such as tinnitus and hyperacusis.
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BibTeX
@article{parker2026tonotopically,
title = {{Tonotopically distinct OFF responses arise in the mouse auditory midbrain following sideband suppression}},
author = {Patrick D. Parker and Amanda M. Lauer and Dwight E. Bergles},
journal = {The Journal of Physiology},
year = {2026},
month = jun,
doi = {10.1113/jp289224},
url = {https://doi.org/10.1113/jp289224},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Tonotopically distinct OFF responses arise in the mouse auditory midbrain following sideband suppression
AU - Patrick D. Parker
AU - Amanda M. Lauer
AU - Dwight E. Bergles
PY - 2026
DA - 2026/06/27
JO - The Journal of Physiology
DO - 10.1113/jp289224
UR - https://doi.org/10.1113/jp289224
AB - The parsing of sensory information into discrete topographic domains is a fundamental principle of sensory processing. In the auditory cortex, these domains evolve during a stimulus, with the onset and offset of tones evoking distinct spatial patterns of neural activity. However, it is not known where in the auditory system this spatial segregation occurs or how these dynamics are affected by hearing loss. Using widefield single photon neuronal Ca2+ imaging in the inferior colliculus (IC) of awake mice, we found that pure tone stimuli elicited both spatially constrained neural activity within isofrequency bands and simultaneous sideband suppression. At cessation of the stimulus, offset responses emerged within the region of sideband suppression, demonstrating that simple stimuli elicit spatiotemporally distinct neural activity patterns to represent the presence of sound and sound termination. Because sound frequency is spatially encoded in the IC, this spatial shift creates a tonotopically distinct offset (tdOFF) response relative to sound onset. High‐resolution two‐photon Ca2+ imaging confirmed that tdOFF neuron activity in the sideband region was suppressed during sound and elevated above baseline after stimulus termination, raising the possibility that rebound excitation could contribute to this post‐stimulus activation. Loud noise exposure – a common model of hearing loss – abolished both sideband suppression and tdOFF responses. These results show that hearing loss profoundly reshapes the spatiotemporal pattern of sound processing by altering sideband activity. This preferential loss of sideband suppression and tdOFF activation after sound‐induced injury in the auditory midbrain may contribute to hyperacusis and tinnitus by promoting neuronal hyperactivity. Key points: Sensory systems encode different features of stimuli by activating distinct neural networks. Sound onsets and offsets elicit distinct neural patterns in the auditory cortex, although it is unclear where this separation originates or how it may change with hearing loss. Using in vivo widefield Ca2+ imaging in awake mice, we find that pure tone stimuli evoke spatiotemporally distinct on and off patterns of neural activity in the auditory midbrain. Neurons active during stimulus offset were suppressed by sound in sideband regions, raising the possibility that rebound excitation may contribute to this post‐stimulus activation. Both sideband suppression and off responses were preferentially abolished following noise‐induced hearing loss, raising the possibility that these changes may contribute to hearing loss‐related syndromes such as tinnitus and hyperacusis.
SN - 0022-3751
SN - 1469-7793
ER -