Arabidopsis nanopore direct RNA sequencing data for mutants in splicing, polyadenylation, and m6A methylation.
8 affiliations
- 1Laboratory of RNA biology, International Institute of Molecular and Cell Biology, 4 Ks. Trojdena, 02-106, Warsaw, Poland.
- 2Department of Gene Expression, Faculty of Biology, Adam Mickiewicz University, 6 Uniwersytetu Poznańskiego, 61-614, Poznań, Poland.
- 3Laboratory of RNA Biology and Molecular Physiology, Faculty of Biology, Bielefeld University, 25 Universitaetsstrasse, 33615, Bielefeld, Germany.
- 4Faculty of Biology, University of Warsaw, 1 Miecznikowa, 02-096, Warsaw, Poland.
- 5Laboratory of RNA biology, International Institute of Molecular and Cell Biology, 4 Ks. Trojdena, 02-106, Warsaw, Poland. [email protected].
- 6Faculty of Biology, University of Warsaw, 1 Miecznikowa, 02-096, Warsaw, Poland. [email protected].
- 7Genome Engineering Facility, IN-MOL-CELL Infrastructure, International Institute of Molecular and Cell Biology, 4 Ks. Trojdena, 02-106, Warsaw, Poland. [email protected].
- 8Department of Gene Expression, Faculty of Biology, Adam Mickiewicz University, 6 Uniwersytetu Poznańskiego, 61-614, Poznań, Poland. [email protected].
Abstract
Co-transcriptional RNA processing, including alternative splicing and polyadenylation, generates diverse mRNA isoforms essential for development, differentiation, and stress responses. We present Oxford Nanopore Direct RNA Sequencing (DRS) data from Arabidopsis thaliana wild-type (Col-0) plants and mutants defective in RNA processing pathways, including polyadenylation, RNA methylation, and pre-mRNA splicing. We obtained full-length reads together with native poly(A) tail information. The provided resources include FAST5 and FASTQ files, poly(A) tail length estimates from Nanopolish, poly(A) composition data from Ninetails, and differential expression analyses generated with DESeq2, all aligned to the TAIR10 reference transcriptome. These data offer a comprehensive view of RNA processing at the single-molecule level and can be reused to explore alternative polyadenylation, RNA modification patterns, or indirectly, splicing alterations across various genetic backgrounds. This resource is intended to support studies of gene expression regulation and RNA metabolism in Arabidopsis thaliana and related plant systems.
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BibTeX
@article{guminska2026arabidopsis,
title = {{Arabidopsis nanopore direct RNA sequencing data for mutants in splicing, polyadenylation, and m6A methylation.}},
author = {Natalia Gumińska and Łukasz Szewc and Agnieszka Czarnocka-Cieciura and Wiktoria Orzeł and Sebastian Jeleń and Mateusz Bajczyk and Seweryn Mroczek and Andrzej Dziembowski and Artur Jarmołowski},
journal = {Scientific data},
year = {2026},
month = oct,
doi = {10.1038/s41597-026-07757-4},
url = {https://europepmc.org/article/MED/42897175},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Arabidopsis nanopore direct RNA sequencing data for mutants in splicing, polyadenylation, and m6A methylation.
AU - Natalia Gumińska
AU - Łukasz Szewc
AU - Agnieszka Czarnocka-Cieciura
AU - Wiktoria Orzeł
AU - Sebastian Jeleń
AU - Mateusz Bajczyk
AU - Seweryn Mroczek
AU - Andrzej Dziembowski
AU - Artur Jarmołowski
PY - 2026
DA - 2026/10/19
JO - Scientific data
DO - 10.1038/s41597-026-07757-4
UR - https://europepmc.org/article/MED/42897175
AB - Co-transcriptional RNA processing, including alternative splicing and polyadenylation, generates diverse mRNA isoforms essential for development, differentiation, and stress responses. We present Oxford Nanopore Direct RNA Sequencing (DRS) data from Arabidopsis thaliana wild-type (Col-0) plants and mutants defective in RNA processing pathways, including polyadenylation, RNA methylation, and pre-mRNA splicing. We obtained full-length reads together with native poly(A) tail information. The provided resources include FAST5 and FASTQ files, poly(A) tail length estimates from Nanopolish, poly(A) composition data from Ninetails, and differential expression analyses generated with DESeq2, all aligned to the TAIR10 reference transcriptome. These data offer a comprehensive view of RNA processing at the single-molecule level and can be reused to explore alternative polyadenylation, RNA modification patterns, or indirectly, splicing alterations across various genetic backgrounds. This resource is intended to support studies of gene expression regulation and RNA metabolism in Arabidopsis thaliana and related plant systems.
SN - 2052-4463
ER -