Overexpression of PlPAT1, a GRAS transcription factor from Paeonia lactiflora, enhances salt stress tolerance in transgenic tobacco.
- 1School of Biology and Food Engineering, Fuyang Normal University, Fuyang, Anhui, China.
- 2School of Biology and Food Engineering, Fuyang Normal University, Fuyang, Anhui, China. [email protected].
- 3Anhui Huatuo Academy of Traditional Chinese Medicine, Bozhou Vocational and Technical College, Bozhou, Anhui, China. [email protected].
Abstract
Background
Soil salinization poses a significant threat to agricultural productivity worldwide, including the cultivation of Paeonia lactiflora. Understanding the molecular mechanisms of salt stress response and identifying key salt-tolerant genes are essential for breeding resilient varieties. The GRAS family of transcription factors is known to regulate plant growth, development, and abiotic stress responses; however, its role in P. lactiflora remains poorly characterized.Results
In this study, we identified a novel GRAS transcription factor from P. lactiflora, designated PlPAT1, based on its homology to Arabidopsis GRAS members. Under salt stress, PlPAT1 expression was up-regulated in leaves and stems but down-regulated in roots. Subcellular localization revealed that PlPAT1 protein localizes to both the nucleus and cytoplasm. Transgenic tobacco lines overexpressing PlPAT1 exhibited enhanced salt tolerance, accompanied by increased chlorophyll content, elevated levels of soluble sugars and proteins, and higher activities of antioxidant enzymes (SOD, POD, CAT). In contrast, malondialdehyde (MDA) and hydrogen peroxide accumulation were significantly reduced compared to wild-type plants.Conclusion
Our results demonstrate that PlPAT1 functions as a positive regulator of salt stress tolerance, likely through modulating osmotic balance and enhancing reactive oxygen species scavenging capacity. These findings highlight the potential of PlPAT1 as a candidate gene for improving salt tolerance in plants and provide a genetic resource for the utilization of saline-alkali land.
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BibTeX
@article{cai2026overexpression,
title = {{Overexpression of PlPAT1, a GRAS transcription factor from Paeonia lactiflora, enhances salt stress tolerance in transgenic tobacco.}},
author = {Jian Cai and Xuemei Zhang and Cong Yan and Chao Lu and Zhongxuan Fu and Yaowu Liu and Gang Li},
journal = {BMC genomics},
year = {2026},
month = aug,
doi = {10.1186/s12864-026-13204-w},
url = {https://europepmc.org/article/MED/42958050},
note = {CC BY},
}
RIS
TY - JOUR
TI - Overexpression of PlPAT1, a GRAS transcription factor from Paeonia lactiflora, enhances salt stress tolerance in transgenic tobacco.
AU - Jian Cai
AU - Xuemei Zhang
AU - Cong Yan
AU - Chao Lu
AU - Zhongxuan Fu
AU - Yaowu Liu
AU - Gang Li
PY - 2026
DA - 2026/08/01
JO - BMC genomics
DO - 10.1186/s12864-026-13204-w
UR - https://europepmc.org/article/MED/42958050
AB - Background Soil salinization poses a significant threat to agricultural productivity worldwide, including the cultivation of Paeonia lactiflora. Understanding the molecular mechanisms of salt stress response and identifying key salt-tolerant genes are essential for breeding resilient varieties. The GRAS family of transcription factors is known to regulate plant growth, development, and abiotic stress responses; however, its role in P. lactiflora remains poorly characterized.Results In this study, we identified a novel GRAS transcription factor from P. lactiflora, designated PlPAT1, based on its homology to Arabidopsis GRAS members. Under salt stress, PlPAT1 expression was up-regulated in leaves and stems but down-regulated in roots. Subcellular localization revealed that PlPAT1 protein localizes to both the nucleus and cytoplasm. Transgenic tobacco lines overexpressing PlPAT1 exhibited enhanced salt tolerance, accompanied by increased chlorophyll content, elevated levels of soluble sugars and proteins, and higher activities of antioxidant enzymes (SOD, POD, CAT). In contrast, malondialdehyde (MDA) and hydrogen peroxide accumulation were significantly reduced compared to wild-type plants.Conclusion Our results demonstrate that PlPAT1 functions as a positive regulator of salt stress tolerance, likely through modulating osmotic balance and enhancing reactive oxygen species scavenging capacity. These findings highlight the potential of PlPAT1 as a candidate gene for improving salt tolerance in plants and provide a genetic resource for the utilization of saline-alkali land.
SN - 1471-2164
ER -