A multifunctional bishydroxycoumarin conjugate exhibiting AIE characteristics and tandem Co²⁺/ATP detection with logic gate operations, on-site sensing and bioimaging applications.
- 1Department of Chemistry, Bharathiar University, 641046 Coimbatore Tamil Nadu India.
- 2Department of Biotechnology, Bharathiar University, 641046 Coimbatore Tamil Nadu India [email protected] [email protected].
Abstract
An efficient coumarin-based fluorescent probe, HCPD, was synthesized via condensation of 7-diethylamino-4-hydroxycoumarin aldehyde with o-phenylenediamine. The structure of the probe was confirmed by FT-IR, NMR and HRMS studies. The probe, HCPD exhibited aggregation-induced emission (AIE) characteristics in a THF-water mixture, showing enhanced emission at 80% water fraction. The probe functions as a molecular switch for the sensitive detection of the environmentally and biologically significant metal ion Co²⁺ at the nanomolar level. HCPD emits green emission at 500 nm which selectively detects Co²⁺ ions in a DMF/Tris-HCl buffer system (9 : 1, v/v) through an emission "turn-off" response, attributed to paramagnetic quenching. In addition, Job's plot and HRMS analysis established a 1 : 1 binding stoichiometry between HCPD and Co²⁺ ions. The probe demonstrated remarkable sensitivity with a low detection limit of 11.7 nM and a binding constant of 3.6798 × 10⁴ M⁻¹. Notably, the emission of the HCPD-Co²⁺ ensemble was restored upon the addition of ATP over various competing anions, enabling reversible sensing behavior not previously reported in the reversibility of Co²⁺ ions. The regeneration capability of the probe facilitated practical on-site applications, including paper strips, cotton swabs and the construction of an IMPLICATION logic gate circuit. Furthermore, HCPD was also successfully applied in bio-imaging studies of MDA-MB-231 breast cancer cell lines, highlighting its biological relevance. Overall, HCPD represents a highly sensitive and reversible dual-responsive fluorescent probe for Co²⁺ and ATP detection with significant environmental and biological applications.
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BibTeX
@article{kumar2026multifunctional,
title = {{A multifunctional bishydroxycoumarin conjugate exhibiting AIE characteristics and tandem Co²⁺/ATP detection with logic gate operations, on-site sensing and bioimaging applications.}},
author = {Rajendran Kishore Kumar and Suryaa Manoharan and Ekambaram Perumal and Balasubramanian Murugesapandian},
journal = {RSC advances},
year = {2026},
month = oct,
doi = {10.1039/d6ra08250e},
url = {https://europepmc.org/article/MED/42899141},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - A multifunctional bishydroxycoumarin conjugate exhibiting AIE characteristics and tandem Co²⁺/ATP detection with logic gate operations, on-site sensing and bioimaging applications.
AU - Rajendran Kishore Kumar
AU - Suryaa Manoharan
AU - Ekambaram Perumal
AU - Balasubramanian Murugesapandian
PY - 2026
DA - 2026/10/19
JO - RSC advances
DO - 10.1039/d6ra08250e
UR - https://europepmc.org/article/MED/42899141
AB - An efficient coumarin-based fluorescent probe, HCPD, was synthesized via condensation of 7-diethylamino-4-hydroxycoumarin aldehyde with o-phenylenediamine. The structure of the probe was confirmed by FT-IR, NMR and HRMS studies. The probe, HCPD exhibited aggregation-induced emission (AIE) characteristics in a THF-water mixture, showing enhanced emission at 80% water fraction. The probe functions as a molecular switch for the sensitive detection of the environmentally and biologically significant metal ion Co²⁺ at the nanomolar level. HCPD emits green emission at 500 nm which selectively detects Co²⁺ ions in a DMF/Tris-HCl buffer system (9 : 1, v/v) through an emission "turn-off" response, attributed to paramagnetic quenching. In addition, Job's plot and HRMS analysis established a 1 : 1 binding stoichiometry between HCPD and Co²⁺ ions. The probe demonstrated remarkable sensitivity with a low detection limit of 11.7 nM and a binding constant of 3.6798 × 10⁴ M⁻¹. Notably, the emission of the HCPD-Co²⁺ ensemble was restored upon the addition of ATP over various competing anions, enabling reversible sensing behavior not previously reported in the reversibility of Co²⁺ ions. The regeneration capability of the probe facilitated practical on-site applications, including paper strips, cotton swabs and the construction of an IMPLICATION logic gate circuit. Furthermore, HCPD was also successfully applied in bio-imaging studies of MDA-MB-231 breast cancer cell lines, highlighting its biological relevance. Overall, HCPD represents a highly sensitive and reversible dual-responsive fluorescent probe for Co²⁺ and ATP detection with significant environmental and biological applications.
SN - 2046-2069
ER -