Amplifying gene expression sensitivity by surface condensation on DNA
Abstract
Condensation of proteins on DNA is a central aspect of genome organisation, and has been proposed as a mechanism of gene regulation in eukaryotes, notably by mediating the action of distal enhancers on promoter activity. However, the physics of this interaction and its impact on the precision of gene regulation remain to be fully elucidated. Here, we study a simplified system of two regulatory regions of the DNA, a distal enhancer and a promoter, with affinity to a self-attracting ligand that effectively binds them together. Using 3-dimensional lattice simulations and a mean-field theory of the ligand-water mixture coupled to a Rouse polymer, we show that small differences in ligand concentrations can be greatly amplified at the level of transcriptional activity compared to a free ligand receptor. We quantify this sensitivity using the Fisher information rate, which can be decomposed into two contributions. First, the formation of a pre-wet layer of ligands around regulatory regions increases ligand concentrations at the promoter site, enhancing concentration differences. Second, the DNA polymer itself switches between a closed state, where enhancer and promoter are in contact through an attractive capillary bridge formed by ligands, and an open state where they are apart. That attractive force determines the typical dwell time of the closed configuration where the gene is most active, and strongly depends on the ligand bulk concentration, contributing to the sensitivity of gene expression. These results suggest a general mechanism and theoretical framework for the precise regulation of genes through tunable genome organization.
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BibTeX
@misc{tohme2026amplifying,
title = {{Amplifying gene expression sensitivity by surface condensation on DNA}},
author = {Tarek Tohme and Henry Alston and Jacqueline Janssen and Aleksandra M Walczak and Thierry Mora},
howpublished = {arXiv},
year = {2026},
month = oct,
doi = {10.48550/arxiv.2610.22667},
eprint = {2610.22667},
archivePrefix = {arXiv},
url = {https://arxiv.org/abs/2610.22667},
}
RIS
TY - UNPB
TI - Amplifying gene expression sensitivity by surface condensation on DNA
AU - Tarek Tohme
AU - Henry Alston
AU - Jacqueline Janssen
AU - Aleksandra M Walczak
AU - Thierry Mora
PY - 2026
DA - 2026/10/15
PB - arXiv
DO - 10.48550/arxiv.2610.22667
UR - https://arxiv.org/abs/2610.22667
AB - Condensation of proteins on DNA is a central aspect of genome organisation, and has been proposed as a mechanism of gene regulation in eukaryotes, notably by mediating the action of distal enhancers on promoter activity. However, the physics of this interaction and its impact on the precision of gene regulation remain to be fully elucidated. Here, we study a simplified system of two regulatory regions of the DNA, a distal enhancer and a promoter, with affinity to a self-attracting ligand that effectively binds them together. Using 3-dimensional lattice simulations and a mean-field theory of the ligand-water mixture coupled to a Rouse polymer, we show that small differences in ligand concentrations can be greatly amplified at the level of transcriptional activity compared to a free ligand receptor. We quantify this sensitivity using the Fisher information rate, which can be decomposed into two contributions. First, the formation of a pre-wet layer of ligands around regulatory regions increases ligand concentrations at the promoter site, enhancing concentration differences. Second, the DNA polymer itself switches between a closed state, where enhancer and promoter are in contact through an attractive capillary bridge formed by ligands, and an open state where they are apart. That attractive force determines the typical dwell time of the closed configuration where the gene is most active, and strongly depends on the ligand bulk concentration, contributing to the sensitivity of gene expression. These results suggest a general mechanism and theoretical framework for the precise regulation of genes through tunable genome organization.
ER -