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Peer-reviewedCC BYFrontiers in genetics

BCAT1 promotes acetylation of ACLY through fatty acid metabolism pathway promoting the occurrence and development of endometriosis

  • Yanan Xu1
  • Yanping Shen2
  • Yingyan Yan3
  • Liang Chen4
4 affiliations
  1. 1Department of Scientific Research, The Affiliated Women and Children’s Hospital of Ningbo University , , ,
  2. 2Department of Anesthesiology, The Affiliated Women and Children’s Hospital of Ningbo University , , ,
  3. 3GCP Center, The Affiliated Women and Children’s Hospital of Ningbo University , , ,
  4. 4Department of Gynecology, The Affiliated Women and Children’s Hospital of Ningbo University , , ,
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Abstract

Objective

Currently, surgical intervention is the primary treatment for endometriosis (EM), but recurrence is common. This study aims to identify novel potential therapeutic targets for effective management of EM by investigating the role of branched chain amino acid transaminase 1 (BCAT1).Methods

We analyzed BCAT1 expression in EM tissues using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis. The functional impact of BCAT1 on ectopic endometrial stromal cells (EESCs) was evaluated using CCK-8, colony formation, wound healing, and Transwell assays. To study BCAT1's role in fatty acid metabolism, we conducted transcriptomic sequencing, oxygen consumption rate (OCR) assays, lipid droplet assays, and measured cholesterol and triglyceride levels. The interaction between BCAT1 and ATP-citrate lyase (ACLY) was further analyzed through Western blotting and BCAT1 modulation experiments.Results

BCAT1 expression was significantly elevated in EM tissues. Functional assays demonstrated that BCAT1 promotes the proliferation, migration, and invasion of EESCs in vitro. BCAT1 also positively regulates fatty acid metabolism, which enhances ACLY expression and contributes to cell proliferation. Mechanistically, BCAT1 influences ACLY expression through epigenetic regulation, specifically via histone acetylation.Conclusion

BCAT1 plays a crucial role in regulating EESC functions by modulating fatty acid metabolism and ACLY expression. These findings provide a theoretical basis for EM treatment.

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Cite

BibTeXRIS
BibTeX
@article{xu2026bcat1,
  title = {{BCAT1 promotes acetylation of ACLY through fatty acid metabolism pathway promoting the occurrence and development of endometriosis}},
  author = {Yanan Xu and Yanping Shen and Yingyan Yan and Liang Chen},
  journal = {Frontiers in genetics},
  year = {2026},
  month = oct,
  doi = {10.3389/fgene.2026.1818692},
  url = {https://europepmc.org/article/PMC/PMC13727071},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - BCAT1 promotes acetylation of ACLY through fatty acid metabolism pathway promoting the occurrence and development of endometriosis
AU  - Yanan Xu
AU  - Yanping Shen
AU  - Yingyan Yan
AU  - Liang Chen
PY  - 2026
DA  - 2026/10/20
JO  - Frontiers in genetics
DO  - 10.3389/fgene.2026.1818692
UR  - https://europepmc.org/article/PMC/PMC13727071
AB  - Objective  Currently, surgical intervention is the primary treatment for endometriosis (EM), but recurrence is common. This study aims to identify novel potential therapeutic targets for effective management of EM by investigating the role of branched chain amino acid transaminase 1 (BCAT1).Methods  We analyzed BCAT1 expression in EM tissues using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis. The functional impact of BCAT1 on ectopic endometrial stromal cells (EESCs) was evaluated using CCK-8, colony formation, wound healing, and Transwell assays. To study BCAT1's role in fatty acid metabolism, we conducted transcriptomic sequencing, oxygen consumption rate (OCR) assays, lipid droplet assays, and measured cholesterol and triglyceride levels. The interaction between BCAT1 and ATP-citrate lyase (ACLY) was further analyzed through Western blotting and BCAT1 modulation experiments.Results  BCAT1 expression was significantly elevated in EM tissues. Functional assays demonstrated that BCAT1 promotes the proliferation, migration, and invasion of EESCs in vitro. BCAT1 also positively regulates fatty acid metabolism, which enhances ACLY expression and contributes to cell proliferation. Mechanistically, BCAT1 influences ACLY expression through epigenetic regulation, specifically via histone acetylation.Conclusion  BCAT1 plays a crucial role in regulating EESC functions by modulating fatty acid metabolism and ACLY expression. These findings provide a theoretical basis for EM treatment.
SN  - 1664-8021
ER  -