Characterization of ovine granulosa cell-derived extracellular vesicles and their miRNA cargo following in vitro exposure to bisphenols A and S
Abstract
Bisphenol A (BPA) and Bisphenol S (BPS) exposure disrupt ovarian function and granulosa cell (GC) steroidogenesis. Extracellular vesicles (EVs) and their miRNA cargo, as mediators of cellular response to environmental stimuli, might be involved in fertility and folliculogenesis. This study explored modulation of microRNA (miRNA) expression after 48 h BPA or BPS exposure (10 µM) in ovine primary GC and EVs from corresponding conditioned medium (CM EVs). Small RNA sequencing of control (0 h) and 48 h treated GC, CM EVs allowed identification of 526 ovine miRNAs, including 128 new sequences. BPA did not alter miRNA expression in GC, while BPS decreased cellular oar-24b miRNA. In contrast, BPA modified expression of 4 miRNAs in CM EVs, including 3 new sequences, and 2 miRNAs were modified by BPS. Both compounds reduced expression of a sequence homologous to miR-1306. Further studies are required to decipher their roles in bisphenol toxicity in GC.
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BibTeX
@article{desmarchais2026characterization,
title = {{Characterization of ovine granulosa cell-derived extracellular vesicles and their miRNA cargo following in vitro exposure to bisphenols A and S}},
author = {Alice Desmarchais and Svetlana Uzbekova and Virginie Maillard and Pascal Papillier and Cécile Douet and Thomas Duret and Rustem Uzbekov and Quentin Meffre and Benoît Piégu and Gaëlle Lefort and Noémie Teixido and Anaïs Vitorino Carvalho and Sébastien Roger and Sébastien Elis},
journal = {Environmental Toxicology and Pharmacology},
year = {2026},
month = jul,
doi = {10.1016/j.etap.2026.105117},
url = {https://hal.inrae.fr/hal-05707039v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Characterization of ovine granulosa cell-derived extracellular vesicles and their miRNA cargo following in vitro exposure to bisphenols A and S
AU - Alice Desmarchais
AU - Svetlana Uzbekova
AU - Virginie Maillard
AU - Pascal Papillier
AU - Cécile Douet
AU - Thomas Duret
AU - Rustem Uzbekov
AU - Quentin Meffre
AU - Benoît Piégu
AU - Gaëlle Lefort
AU - Noémie Teixido
AU - Anaïs Vitorino Carvalho
AU - Sébastien Roger
AU - Sébastien Elis
PY - 2026
DA - 2026/07/25
JO - Environmental Toxicology and Pharmacology
DO - 10.1016/j.etap.2026.105117
UR - https://hal.inrae.fr/hal-05707039v1
AB - Bisphenol A (BPA) and Bisphenol S (BPS) exposure disrupt ovarian function and granulosa cell (GC) steroidogenesis. Extracellular vesicles (EVs) and their miRNA cargo, as mediators of cellular response to environmental stimuli, might be involved in fertility and folliculogenesis. This study explored modulation of microRNA (miRNA) expression after 48 h BPA or BPS exposure (10 µM) in ovine primary GC and EVs from corresponding conditioned medium (CM EVs). Small RNA sequencing of control (0 h) and 48 h treated GC, CM EVs allowed identification of 526 ovine miRNAs, including 128 new sequences. BPA did not alter miRNA expression in GC, while BPS decreased cellular oar-24b miRNA. In contrast, BPA modified expression of 4 miRNAs in CM EVs, including 3 new sequences, and 2 miRNAs were modified by BPS. Both compounds reduced expression of a sequence homologous to miR-1306. Further studies are required to decipher their roles in bisphenol toxicity in GC.
SN - 1382-6689
ER -