Frequency of macrolide resistance-associated mutations in Mycoplasma genitalium: a multicenter molecular analysis based in Brazil.
6 affiliations
- 1Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Laboratório de Biologia Molecular, Microbiologia e Sorologia, Florianópolis, Santa Catarina, Brazil.
- 2Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Biotecnologia e Biociências, Laboratório de Bioinformática, Florianópolis, Santa Catarina, Brazil.
- 3Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Biotecnologia e Biociências, Laboratório de Virologia Aplicada, Florianópolis, Santa Catarina, Brazil.
- 4Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Farmácia, Laboratório de Biologia Molecular, Microbiologia e Sorologia, Florianópolis, Santa Catarina, Brazil.
- 5Ministério da Saúde, Departamento de HIV/AIDS, Tuberculose, Hepatites Virais e Infecções Sexualmente Transmissíveis, Brasília, Distrito Federal, Brazil.
- 6Universidade Federal do Espírito Santo, Programa de Pós-Graduação em Doenças Infecciosas, Vitória, Espírito Santo, Brazil.
Abstract
Mycoplasma genitalium is an emerging sexually transmitted pathogen with increasing antimicrobial resistance worldwide. In Brazil, molecular testing for M. genitalium and resistance-associated mutations is not routinely available, and national data remain scarce. We analyzed 118 M. genitalium-positive urethral and vaginal swab samples collected between 2018 and 2023 from sites across all five Brazilian geographic regions. PCR amplification and sequencing of 23S rRNA, parC, and gyrA identified macrolide and fluoroquinolone resistance-associated mutations. Among 79 samples successfully sequenced for 23S rRNA, 40.5% harbored macrolide resistance-associated mutations (A2058G, A2059G, or A2058T) detected in samples from all five regions at different frequencies. A total of 6.1% of sequenced samples presented parC mutations, including two with combined 23S rRNA and parC mutations. No resistance-associated mutations were detected in gyrA. While resistance-associated mutations occurred in both symptomatic men and pregnant women, these findings are not representative estimates for the Brazilian population since the study used convenience samples from two distinct surveillance populations. Nonetheless, they provide multicenter molecular data that may inform future surveillance, diagnostic strategies, antimicrobial stewardship, and resistance-guided management of M. genitalium in Brazil.
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BibTeX
@article{barazzetti2026frequency,
title = {{Frequency of macrolide resistance-associated mutations in Mycoplasma genitalium: a multicenter molecular analysis based in Brazil.}},
author = {Fernando Hartmann Barazzetti and Marcos André Schörner and Vilmar Benetti Filho and Henrique Borges da Silva Grisard and Julia Kinetz Wachter and Jéssica Motta Martins and Christinni Machado Venturi and Pâmela Cristina Gaspar and Angélica Espinosa Miranda and Dráurio Barreira and Glauber Wagner and Maria Luiza Bazzo},
journal = {Revista do Instituto de Medicina Tropical de Sao Paulo},
year = {2026},
month = oct,
doi = {10.1590/s1678-9946202668070},
url = {https://europepmc.org/article/MED/42912016},
note = {CC BY},
}
RIS
TY - JOUR
TI - Frequency of macrolide resistance-associated mutations in Mycoplasma genitalium: a multicenter molecular analysis based in Brazil.
AU - Fernando Hartmann Barazzetti
AU - Marcos André Schörner
AU - Vilmar Benetti Filho
AU - Henrique Borges da Silva Grisard
AU - Julia Kinetz Wachter
AU - Jéssica Motta Martins
AU - Christinni Machado Venturi
AU - Pâmela Cristina Gaspar
AU - Angélica Espinosa Miranda
AU - Dráurio Barreira
AU - Glauber Wagner
AU - Maria Luiza Bazzo
PY - 2026
DA - 2026/10/19
JO - Revista do Instituto de Medicina Tropical de Sao Paulo
DO - 10.1590/s1678-9946202668070
UR - https://europepmc.org/article/MED/42912016
AB - Mycoplasma genitalium is an emerging sexually transmitted pathogen with increasing antimicrobial resistance worldwide. In Brazil, molecular testing for M. genitalium and resistance-associated mutations is not routinely available, and national data remain scarce. We analyzed 118 M. genitalium-positive urethral and vaginal swab samples collected between 2018 and 2023 from sites across all five Brazilian geographic regions. PCR amplification and sequencing of 23S rRNA, parC, and gyrA identified macrolide and fluoroquinolone resistance-associated mutations. Among 79 samples successfully sequenced for 23S rRNA, 40.5% harbored macrolide resistance-associated mutations (A2058G, A2059G, or A2058T) detected in samples from all five regions at different frequencies. A total of 6.1% of sequenced samples presented parC mutations, including two with combined 23S rRNA and parC mutations. No resistance-associated mutations were detected in gyrA. While resistance-associated mutations occurred in both symptomatic men and pregnant women, these findings are not representative estimates for the Brazilian population since the study used convenience samples from two distinct surveillance populations. Nonetheless, they provide multicenter molecular data that may inform future surveillance, diagnostic strategies, antimicrobial stewardship, and resistance-guided management of M. genitalium in Brazil.
SN - 0036-4665
SN - 1678-9946
ER -