Accessing Polyphenols Effect on Bond Strength and Dentin Enzymatic Degradation
- 1Faculty of Dentistry Universidade Federal de Alagoas Maceió Alagoas Brazil
Abstract
Introduction Polyphenols have been investigated in dental materials and restorative techniques due to their interactions with metal cations, antioxidant activity, and collagen crosslinking ability, which make them a promise for reinforcing the dental substrate and improving material adhesion. However, more studies are necessary before to support their use in clinical procedures. Objectives This study evaluated the effects of quercetin and resveratrol on bond strength and the protection of dentin collagen fibrils against enzymatic degradation. Materials and Methods Demineralized human dentin specimens were immersed for 1 min in solutions of quercetin or resveratrol (100, 250, and 500 µg/mL), 2% chlorhexidine (CHX), or 0.9% saline (control). The specimens were then incubated in collagenase for 24 h at 37°C, and changes in dry mass were measured. For microtensile bond strength testing, quercetin, resveratrol, CHX, or distilled water (DW) were applied to phosphoric acid–conditioned dentin, followed by application of Single Bond 2 (3 M/ESPE) adhesive and Z350XT resin (3 M/ESPE). Adhesive strength was measured after 24 h and after 1 year in distilled water. Data were analyzed using the Kruskal–Wallis and Dunn tests or ANOVA followed by Tukey's test (p < 0.05). Results Quercetin‐treated specimens showed a significant weight increase compared with CHX (p < 0.05). Both quercetin‐ and resveratrol‐treated groups exhibited resistance to collagenase similar to CHX (p > 0.05). After 1 year, only the DW group showed a significant decrease in bond strength (p = 0.001). Conclusions Dentin treatment with quercetin or resveratrol effectively preserved adhesive strength and protected collagen against enzymatic degradation, suggesting their potential as agents to improve the longevity of adhesive restorations.
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BibTeX
@article{alves2026accessing,
title = {{Accessing Polyphenols Effect on Bond Strength and Dentin Enzymatic Degradation}},
author = {Ana Catarina Rios Castro Alves and Adonias Antonio da Silva and Roberta Bruno da Silva and Rayan deLima França and Dayse Andrade Romão and deFragoso and Natanael Barbosa dos Santos and dePorto},
journal = {Clinical and Experimental Dental Research},
year = {2026},
month = oct,
doi = {10.1002/cre2.70449},
url = {https://doi.org/10.1002/cre2.70449},
note = {CC BY},
}
RIS
TY - JOUR
TI - Accessing Polyphenols Effect on Bond Strength and Dentin Enzymatic Degradation
AU - Ana Catarina Rios Castro Alves
AU - Adonias Antonio da Silva
AU - Roberta Bruno da Silva
AU - Rayan deLima França
AU - Dayse Andrade Romão
AU - deFragoso
AU - Natanael Barbosa dos Santos
AU - dePorto
PY - 2026
DA - 2026/10/30
JO - Clinical and Experimental Dental Research
DO - 10.1002/cre2.70449
UR - https://doi.org/10.1002/cre2.70449
AB - Introduction Polyphenols have been investigated in dental materials and restorative techniques due to their interactions with metal cations, antioxidant activity, and collagen crosslinking ability, which make them a promise for reinforcing the dental substrate and improving material adhesion. However, more studies are necessary before to support their use in clinical procedures. Objectives This study evaluated the effects of quercetin and resveratrol on bond strength and the protection of dentin collagen fibrils against enzymatic degradation. Materials and Methods Demineralized human dentin specimens were immersed for 1 min in solutions of quercetin or resveratrol (100, 250, and 500 µg/mL), 2% chlorhexidine (CHX), or 0.9% saline (control). The specimens were then incubated in collagenase for 24 h at 37°C, and changes in dry mass were measured. For microtensile bond strength testing, quercetin, resveratrol, CHX, or distilled water (DW) were applied to phosphoric acid–conditioned dentin, followed by application of Single Bond 2 (3 M/ESPE) adhesive and Z350XT resin (3 M/ESPE). Adhesive strength was measured after 24 h and after 1 year in distilled water. Data were analyzed using the Kruskal–Wallis and Dunn tests or ANOVA followed by Tukey's test (p < 0.05). Results Quercetin‐treated specimens showed a significant weight increase compared with CHX (p < 0.05). Both quercetin‐ and resveratrol‐treated groups exhibited resistance to collagenase similar to CHX (p > 0.05). After 1 year, only the DW group showed a significant decrease in bond strength (p = 0.001). Conclusions Dentin treatment with quercetin or resveratrol effectively preserved adhesive strength and protected collagen against enzymatic degradation, suggesting their potential as agents to improve the longevity of adhesive restorations.
SN - 2057-4347
ER -