High-resolution characterization of classical and non-classical HLA diversity reveals important admixture in Brazilian indigenous.
- 1Hospital Israelita Albert Einstein, São Paulo, Brazil.
Abstract
Understanding linkage disequilibrium (LD) patterns involving both classical and non-classical HLA genes across diverse ethnic backgrounds provides valuable insights into genomic block inheritance, ancestry inference in admixed populations, and the genetic architecture of immune-mediated diseases. We performed high-resolution typing of classical and non-classical HLA genes using next-generation sequencing in 80 Brazilian Indigenous from 24 families belonging to 24 distinct geographical indigenous communities. Gene copy number analysis was conducted in hemizygous and homozygous samples to confirm zygosity. Allelic and haplotypic frequencies, as well as Hardy-Weinberg equilibrium, were assessed in 50 unrelated individuals, while haplotypes were manually inferred through familial segregation analysis in the remaining families. Extensive allelic and haplotypic diversity was observed, with limited sharing across families and several haplotypes not previously described in the Brazilian population. In addition, haplotypes commonly reported in other ethnic groups were identified, likely reflecting the high degree of genetic admixture in Brazil. These findings contribute to the inclusion of Brazilian Indigenous populations in high-resolution immunogenetic studies and may have implications for ancestry inference, disease association analyses, and donor selection strategies in transplantation.
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BibTeX
@article{centuriao2026highresolution,
title = {{High-resolution characterization of classical and non-classical HLA diversity reveals important admixture in Brazilian indigenous.}},
author = {Newton De Freitas Centurião and Lidia Cardoso Francisco and Manuel Mindlin Lafer and Roberta Sitnik and João Renato Rebello Pinho and Giselle Lima Groetares and Elena Outon Alonso and Gabriella Camerini Maciel and Margareth Afonso Torres},
journal = {Frontiers in genetics},
year = {2026},
month = oct,
doi = {10.3389/fgene.2026.1948784},
url = {https://europepmc.org/article/MED/42923279},
note = {CC BY},
}
RIS
TY - JOUR
TI - High-resolution characterization of classical and non-classical HLA diversity reveals important admixture in Brazilian indigenous.
AU - Newton De Freitas Centurião
AU - Lidia Cardoso Francisco
AU - Manuel Mindlin Lafer
AU - Roberta Sitnik
AU - João Renato Rebello Pinho
AU - Giselle Lima Groetares
AU - Elena Outon Alonso
AU - Gabriella Camerini Maciel
AU - Margareth Afonso Torres
PY - 2026
DA - 2026/10/08
JO - Frontiers in genetics
DO - 10.3389/fgene.2026.1948784
UR - https://europepmc.org/article/MED/42923279
AB - Understanding linkage disequilibrium (LD) patterns involving both classical and non-classical HLA genes across diverse ethnic backgrounds provides valuable insights into genomic block inheritance, ancestry inference in admixed populations, and the genetic architecture of immune-mediated diseases. We performed high-resolution typing of classical and non-classical HLA genes using next-generation sequencing in 80 Brazilian Indigenous from 24 families belonging to 24 distinct geographical indigenous communities. Gene copy number analysis was conducted in hemizygous and homozygous samples to confirm zygosity. Allelic and haplotypic frequencies, as well as Hardy-Weinberg equilibrium, were assessed in 50 unrelated individuals, while haplotypes were manually inferred through familial segregation analysis in the remaining families. Extensive allelic and haplotypic diversity was observed, with limited sharing across families and several haplotypes not previously described in the Brazilian population. In addition, haplotypes commonly reported in other ethnic groups were identified, likely reflecting the high degree of genetic admixture in Brazil. These findings contribute to the inclusion of Brazilian Indigenous populations in high-resolution immunogenetic studies and may have implications for ancestry inference, disease association analyses, and donor selection strategies in transplantation.
SN - 1664-8021
ER -