Pilot intraindividual secretome profiling of adipose-derived stem cells in multiple symmetric lipomatosis

Abstract

Multiple symmetric lipomatosis (MSL) is a rare adipose tissue disease characterized by progressive, non-encapsulated, symmetrical fat accumulation. Its pathophysiology is still not completely understood. Here, we performed the first intraindividual comparison of secretomes of human adipose-derived stem cells (hASCs) isolated from affected and unaffected regions of n = 15 patients (proteomic discovery cohort n = 5). Affected MSL-hASCs tend to show accelerated metabolic signals and higher cell densities with preserved adipogenic differentiation capacity. Secretome transfer of affected MSL-hASCs significantly increased metabolic activity of wt-hASCs after 24 h in 4/7 samples (all explorative Benjamini-Hochberg corrected p = 0.040). Bottom-up proteomic analysis identified 811 proteins, of which 220 were classified as secreted. Thirteen proteins exhibited nominally significant dysregulation of expression, including CALR ( p = 0.0061), LAMC1 ( p = 0.0241), S100A7 ( p = 0.0281) and HDGF ( p = 0.0474), but none remained significant after application of the Benjamini-Hochberg (BH) correction to control the False Discovery Rate (FDR) at 0.05. Transcriptional validation by rt-qPCR revealed a trend toward downregulation of hLAMA4 and hCFB (both p = 0.0625). In conclusion, convergent patterns are consistent with extracellular matrix remodeling and PI3K/AKT-ERK-associated proliferative signaling. This model supports a hypothesis of progenitor-level dysregulation rather than a single clonal driver in MSL, which will require further mechanistic validation in larger cohorts.

Multiple Myeloma Cell-Derived Secretome Modulates Bone Marrow Mesenchymal Stromal Cell Migration In Vitro
Maria João Fernandes, Andrea Giestinhas, Sara Reis Moura +4
International journal of molecular sciences30 Sep 2026Peer-reviewedCC BYFull textGeneral medicineEurope PMCCrossref
Atypical spinal epidural lipomatosis: diagnostic strategy and treatment with AUSS technique—A case report
Ke Deng, Leilei Wu, Long Peng +6
Frontiers in surgery16 Oct 2026Peer-reviewedCC BYFull textSurgery & anaesthesiaEurope PMC
Prospective Intraindividual Split-face Histological Comparison of Poly-d,l-lactic Acid and Calcium Hydroxylapatite Skin Boosters Using Stem Cell Markers
Jong Seo Kim
Plastic and reconstructive surgery. Global open1 Oct 2026Peer-reviewedCC BY-NC-NDFull textSurgery & anaesthesiaEurope PMC
Immune Profiling after treatment with an Anti-CD19 Chimeric Antigen Receptor T Cell Therapy in Treatment-Refractory Progressive Multiple Sclerosis
S. Gupta, M. R. Seshadri, R. Dandekar +22
medRxiv11 Sep 2026PreprintAll rights reservedFull textNeurologymedRxiv
NIK stabilization reprograms the pancreas toward chronic pancreatitis and pancreatic lipomatosis
Z. Du, L. Weber, U. F. G. Buettner +8
bioRxiv7 Oct 2026PreprintCC BY-NCFull textOncologybioRxiv

Cite

BibTeX RIS

BibTeX

@article{reinhard2026pilot,
  title = {{Pilot intraindividual secretome profiling of adipose-derived stem cells in multiple symmetric lipomatosis}},
  author = {Ruben Reinhard and Oliver Felthaus and Andreas Siegmund and Katharina Limm and Peter J. Oefner and Marc Ruewe and Silvan Eisenmann and Christian Festbaum and Lukas Prantl and Dmytro Oliinyk},
  journal = {Scientific Reports},
  year = {2026},
  month = oct,
  doi = {10.1038/s41598-026-74618-w},
  url = {https://www.nature.com/articles/s41598-026-74618-w},
  note = {CC BY 4.0},
}

RIS

TY  - JOUR
TI  - Pilot intraindividual secretome profiling of adipose-derived stem cells in multiple symmetric lipomatosis
AU  - Ruben Reinhard
AU  - Oliver Felthaus
AU  - Andreas Siegmund
AU  - Katharina Limm
AU  - Peter J. Oefner
AU  - Marc Ruewe
AU  - Silvan Eisenmann
AU  - Christian Festbaum
AU  - Lukas Prantl
AU  - Dmytro Oliinyk
PY  - 2026
DA  - 2026/10/10
JO  - Scientific Reports
DO  - 10.1038/s41598-026-74618-w
UR  - https://www.nature.com/articles/s41598-026-74618-w
AB  - Multiple symmetric lipomatosis (MSL) is a rare adipose tissue disease characterized by progressive, non-encapsulated, symmetrical fat accumulation. Its pathophysiology is still not completely understood. Here, we performed the first intraindividual comparison of secretomes of human adipose-derived stem cells (hASCs) isolated from affected and unaffected regions of n = 15 patients (proteomic discovery cohort n = 5). Affected MSL-hASCs tend to show accelerated metabolic signals and higher cell densities with preserved adipogenic differentiation capacity. Secretome transfer of affected MSL-hASCs significantly increased metabolic activity of wt-hASCs after 24 h in 4/7 samples (all explorative Benjamini-Hochberg corrected p = 0.040). Bottom-up proteomic analysis identified 811 proteins, of which 220 were classified as secreted. Thirteen proteins exhibited nominally significant dysregulation of expression, including CALR ( p = 0.0061), LAMC1 ( p = 0.0241), S100A7 ( p = 0.0281) and HDGF ( p = 0.0474), but none remained significant after application of the Benjamini-Hochberg (BH) correction to control the False Discovery Rate (FDR) at 0.05. Transcriptional validation by rt-qPCR revealed a trend toward downregulation of hLAMA4 and hCFB (both p = 0.0625). In conclusion, convergent patterns are consistent with extracellular matrix remodeling and PI3K/AKT-ERK-associated proliferative signaling. This model supports a hypothesis of progenitor-level dysregulation rather than a single clonal driver in MSL, which will require further mechanistic validation in larger cohorts.
SN  - 2045-2322
ER  -

Abstract

Related

Cite