Multi-tissue proteome atlas reveals constitutive and reactive cold responses in wild and domesticated chickpea
7 affiliations
- 1Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Perth, WA, Australia; Corresponding author.
- 2Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Perth, WA, Australia
- 3Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Brisbane, QLD, Australia
- 4Curtin Biometry and Agricultural Data Analytics, Centre for Crop and Disease Management, Molecular and Life Sciences, Curtin University, Bentley, WA, Australia
- 5NSW Department of Primary Industries and Regional Development, 4 Marsden Park Road, Tamworth, NSW, Australia
- 6Department of Energy, Environment & Climate Action (DEECA), AgriBio, Centre for AgriBioscience, 5 Ring Road, Bundoora, Victoria, 3083, Australia
- 7The Department of Primary Industries and Regional Development, 14 Monash Gate, Jandakot, WA, 6164, Australia
Abstract
Low temperatures during reproductive development reduce pod set and yield in chickpea, yet the tissue-specific proteome changes leading to yield loss remain largely unresolved. We quantified plant and seed phenotypic traits and generated tissue-resolved proteomes for leaf, flower, and seed from two domesticated cultivars and one wild relative grown under three controlled temperature regimes. Genotype, rather than temperature, was the primary driver of proteome variation in all three tissues, and the wild-domesticated separation persisted across temperature regimes. Wild and domesticated genotypes showed distinct cold-response signatures with no overlapping functional terms. Cold-responsive enzyme inhibitors were enriched in leaf and flower; genotype-specific fructosidase/invertase changes pointed to altered source-tissue sucrose metabolism under cold conditions. Under high day temperature conditions, seed storage protein composition was 11S-dominant in the wild accession and 7S-dominant in both domesticated cultivars. The lowest day temperature partially reversed this balance in PBA Striker through 11S induction. These findings indicate that the proteome separation between wild and domesticated backgrounds is constitutive rather than cold-induced in the genotypes examined.
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BibTeX
@article{onyemaobi2026multitissue,
title = {{Multi-tissue proteome atlas reveals constitutive and reactive cold responses in wild and domesticated chickpea}},
author = {Olive Onyemaobi and Kelley Whisson and Keren Byrne and Sally Stockwell and Jens Berger and Kefei Chen and Kristy Hobson and Muhammad Ahsan Asif and Sukhjiwan Kaur and Zibei Lin and Jane Brownlee and Amanuel Bekuma and Darshan Sharma and Brenton Leske and Michelle L. Colgrave and Cassandra L. Pegg and Utpal Bose},
journal = {Plant Stress},
year = {2026},
month = oct,
doi = {10.1016/j.stress.2026.101572},
url = {https://doi.org/10.1016/j.stress.2026.101572},
note = {CC BY},
}
RIS
TY - JOUR
TI - Multi-tissue proteome atlas reveals constitutive and reactive cold responses in wild and domesticated chickpea
AU - Olive Onyemaobi
AU - Kelley Whisson
AU - Keren Byrne
AU - Sally Stockwell
AU - Jens Berger
AU - Kefei Chen
AU - Kristy Hobson
AU - Muhammad Ahsan Asif
AU - Sukhjiwan Kaur
AU - Zibei Lin
AU - Jane Brownlee
AU - Amanuel Bekuma
AU - Darshan Sharma
AU - Brenton Leske
AU - Michelle L. Colgrave
AU - Cassandra L. Pegg
AU - Utpal Bose
PY - 2026
DA - 2026/10/30
JO - Plant Stress
DO - 10.1016/j.stress.2026.101572
UR - https://doi.org/10.1016/j.stress.2026.101572
AB - Low temperatures during reproductive development reduce pod set and yield in chickpea, yet the tissue-specific proteome changes leading to yield loss remain largely unresolved. We quantified plant and seed phenotypic traits and generated tissue-resolved proteomes for leaf, flower, and seed from two domesticated cultivars and one wild relative grown under three controlled temperature regimes. Genotype, rather than temperature, was the primary driver of proteome variation in all three tissues, and the wild-domesticated separation persisted across temperature regimes. Wild and domesticated genotypes showed distinct cold-response signatures with no overlapping functional terms. Cold-responsive enzyme inhibitors were enriched in leaf and flower; genotype-specific fructosidase/invertase changes pointed to altered source-tissue sucrose metabolism under cold conditions. Under high day temperature conditions, seed storage protein composition was 11S-dominant in the wild accession and 7S-dominant in both domesticated cultivars. The lowest day temperature partially reversed this balance in PBA Striker through 11S induction. These findings indicate that the proteome separation between wild and domesticated backgrounds is constitutive rather than cold-induced in the genotypes examined.
SN - 2667-064X
ER -