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Peer-reviewedCC BYThe Plant journal : for cell and molecular biology

An efficient gene delivery method utilizing the regenerative capacity of alfalfa (Medicago sativa).

  • Jinghui Gao1
  • Houyin Xia1
  • Yajun Wu2
  • Changgen Xie3
  • Jiawen Peng1
  • Yilin Cui1
  • Yiyang Liu1
  • Luna Tan1
  • Chun Li1
  • Qian Li4
  • Jie An1
  • Peizhi Yang1
4 affiliations
  1. 1College of Grassland Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
  2. 2Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, 57007, USA.
  3. 3State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Life Sciences, Northwest A&F University, Yangling, 712100, China.
  4. 4College of Grassland Science, Xinjiang Agricultural University, Urumqi, 830052, China.
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Abstract

Alfalfa (Medicago sativa), an autotetraploid species, is a globally important forage crop. Traditional genetic transformation methods predominantly rely on leaf-derived callus, a process that is complex, inefficient, and particularly challenging to generate stable transgenic lines in polyploid plants. To address these limitations, we developed an efficient transformation system that bypasses callus induction by directly exploiting stem regeneration. Our protocol enables the generation of stable gene-edited alfalfa plants from shoot tips. It accommodates stem explants from various developmental stages and significantly reduces the time required. Moreover, this approach was effective across multiple alfalfa cultivars, and preliminary proof-of-concept experiments in Nicotiana tabacum and Solanum lycopersicum suggested potential transferability beyond alfalfa. This study represents a substantial advancement in perennial plant transformation and provides a robust and practical platform for optimizing stem regeneration-based genetic modification systems.

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Cite

BibTeXRIS
BibTeX
@article{gao2026efficient,
  title = {{An efficient gene delivery method utilizing the regenerative capacity of alfalfa (Medicago sativa).}},
  author = {Jinghui Gao and Houyin Xia and Yajun Wu and Changgen Xie and Jiawen Peng and Yilin Cui and Yiyang Liu and Luna Tan and Chun Li and Qian Li and Jie An and Peizhi Yang},
  journal = {The Plant journal : for cell and molecular biology},
  year = {2026},
  month = oct,
  doi = {10.1111/tpj.71195},
  url = {https://europepmc.org/article/MED/42958684},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - An efficient gene delivery method utilizing the regenerative capacity of alfalfa (Medicago sativa).
AU  - Jinghui Gao
AU  - Houyin Xia
AU  - Yajun Wu
AU  - Changgen Xie
AU  - Jiawen Peng
AU  - Yilin Cui
AU  - Yiyang Liu
AU  - Luna Tan
AU  - Chun Li
AU  - Qian Li
AU  - Jie An
AU  - Peizhi Yang
PY  - 2026
DA  - 2026/10/29
JO  - The Plant journal : for cell and molecular biology
DO  - 10.1111/tpj.71195
UR  - https://europepmc.org/article/MED/42958684
AB  - Alfalfa (Medicago sativa), an autotetraploid species, is a globally important forage crop. Traditional genetic transformation methods predominantly rely on leaf-derived callus, a process that is complex, inefficient, and particularly challenging to generate stable transgenic lines in polyploid plants. To address these limitations, we developed an efficient transformation system that bypasses callus induction by directly exploiting stem regeneration. Our protocol enables the generation of stable gene-edited alfalfa plants from shoot tips. It accommodates stem explants from various developmental stages and significantly reduces the time required. Moreover, this approach was effective across multiple alfalfa cultivars, and preliminary proof-of-concept experiments in Nicotiana tabacum and Solanum lycopersicum suggested potential transferability beyond alfalfa. This study represents a substantial advancement in perennial plant transformation and provides a robust and practical platform for optimizing stem regeneration-based genetic modification systems.
SN  - 0960-7412
SN  - 1365-313X
ER  -