Evaluation of dentinal tubule penetration of a novel nano calcium hydroxide–zinc oxide intracanal medicament: An in vitro pilot study
- 1Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, 162, PH Road, Chennai, 600077, Tamil Nadu, India
- 2Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, 162, PH Road, Chennai, 600077, Tamil Nadu, India; Corresponding author.
- 3Department of Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, Quebec, Canada
Abstract
Background: Residual microorganisms within dentinal tubules remain a key factor in persistent endodontic infections. Therefore, the ability of intracanal medicaments to effectively penetrate these tubules is critical for post-endodontic treatment success and effective long-term prognosis. Aim: To evaluate and compare the dentinal tubule penetration capabilities of a novel nano calcium hydroxide–zinc oxide (NCAL + ZnO) formulation with conventional calcium hydroxide (RC CAL) and triple antibiotic paste (TAP) using confocal laser scanning microscopy (CLSM). Materials and methods: Forty-five extracted single-rooted human teeth were biomechanically prepared using standardized protocols and randomly allocated into three groups (n = 15). Medicaments were labelled with rhodamine B and placed into root canals. Samples were sectioned at coronal, middle, and apical thirds and analyzed using CLSM. Penetration depth (μm) was measured and analyzed using one-way ANOVA and Tukey post hoc test (p < 0.05). Results: RC CAL demonstrated the greatest penetration depths in the coronal and middle thirds, while NCAL + ZnO was the only material showing penetration in the apical third. All intergroup differences were statistically significant (p < 0.001). Conclusion: The novel nano Ca(OH) 2 –ZnO formulation showed a distinct advantage in apical penetration, suggesting potential clinical relevance for improving disinfection in anatomically challenging apical regions.
Related
- Assessment and comparison of coronal discoloration induced by natural derivatives as intracanal medicament: An in vitro observational study
- Micro-CT evaluation of dentin removal in the danger zone and dentinal microcrack formation in primary molars prepared with pediatric rotary file systems: an in vitro study
- In vitro evaluation of N-acetylcysteine, calcium hydroxide, chlorhexidine digluconate and their combinations effect against five dental obligate anaerobic bacteria
- Magnetic Resonance Imaging Evaluation of Azygos Vein Deviation and Risk of Pedicle Screw Penetration in Thoracic Adolescent Idiopathic Scoliosis
- Impact of intracanal cryotherapy on postoperative pain and antimicrobial efficacy in primary teeth: A randomized clinical trial
BibTeX
@article{chowdhury2026evaluation,
title = {{Evaluation of dentinal tubule penetration of a novel nano calcium hydroxide–zinc oxide intracanal medicament: An in vitro pilot study}},
author = {K. Chowdhury and S.D.P. Antony and A Sanghavi and P.K. Elango and P. Solete},
journal = {Journal of Oral Biology and Craniofacial Research},
year = {2026},
month = oct,
doi = {10.1016/j.jobcr.2026.101565},
url = {https://doi.org/10.1016/j.jobcr.2026.101565},
note = {CC BY},
}
RIS
TY - JOUR
TI - Evaluation of dentinal tubule penetration of a novel nano calcium hydroxide–zinc oxide intracanal medicament: An in vitro pilot study
AU - K. Chowdhury
AU - S.D.P. Antony
AU - A Sanghavi
AU - P.K. Elango
AU - P. Solete
PY - 2026
DA - 2026/10/31
JO - Journal of Oral Biology and Craniofacial Research
DO - 10.1016/j.jobcr.2026.101565
UR - https://doi.org/10.1016/j.jobcr.2026.101565
AB - Background: Residual microorganisms within dentinal tubules remain a key factor in persistent endodontic infections. Therefore, the ability of intracanal medicaments to effectively penetrate these tubules is critical for post-endodontic treatment success and effective long-term prognosis. Aim: To evaluate and compare the dentinal tubule penetration capabilities of a novel nano calcium hydroxide–zinc oxide (NCAL + ZnO) formulation with conventional calcium hydroxide (RC CAL) and triple antibiotic paste (TAP) using confocal laser scanning microscopy (CLSM). Materials and methods: Forty-five extracted single-rooted human teeth were biomechanically prepared using standardized protocols and randomly allocated into three groups (n = 15). Medicaments were labelled with rhodamine B and placed into root canals. Samples were sectioned at coronal, middle, and apical thirds and analyzed using CLSM. Penetration depth (μm) was measured and analyzed using one-way ANOVA and Tukey post hoc test (p < 0.05). Results: RC CAL demonstrated the greatest penetration depths in the coronal and middle thirds, while NCAL + ZnO was the only material showing penetration in the apical third. All intergroup differences were statistically significant (p < 0.001). Conclusion: The novel nano Ca(OH) 2 –ZnO formulation showed a distinct advantage in apical penetration, suggesting potential clinical relevance for improving disinfection in anatomically challenging apical regions.
SN - 2212-4268
SN - 2212-4276
ER -