Competing constraints on protein availability and nutrient uptake reshape yeast genetic interactions
- 1NTNU - Norwegian University of Science and Technology
Abstract
Epistasis predicted by enzyme-constrained metabolic models is computed at a single value of the protein available to metabolism, although that amount varies two- to three-fold with growth rate. We ask how epistatic interactions are affected by computing every single and pairwise gene deletion in an enzyme-constrained model of Saccharomyces cerevisiae while sweeping protein availability, with and without a bound on nutrient uptake. Protein availability changes relative fitness only under co-limitation, when the protein pool and a nutrient constraint limit growth together. The pool alone, the configuration in which these models are calibrated, makes protein a mere scale factor: no interaction changes sign. Under co-limitation, one genetic interaction in three is gained or lost across the physiological range. Most of this is inherited from single mutants of the respiratory machinery, which is recruited as protein becomes available. The epistasis between a glycolytic lesion with a protein-expensive bypass and the respiratory chain changes sign where the optimum switches from fermentation to respiration. We also find protein-priced interactions: the network supplies a bypass around each deletion and the pool gives it a price, so two genes with no stoichiometric coupling interact whenever the bypass around one competes with the route of the other for the protein pool. Isozyme pairs predicted neutral by flux balance analysis show negative epistasis for the same reason. Relative fitness depends on protein availability and nutrient supply only through their ratio. Thus, nutrient supply is a directly accessible experimental parameter to test epistatic interactions. Sign inversion, isozyme pairs, and interactions with cofactor-consuming branches are predictions a condition-resolved genetic interaction screen can test.
Related
- Tree species composition and nutrient uptake influence soil nutrient availability, even under contrasted levels of water availability
- Rural food markets and their implications for the availability and affordability of nutrient-dense foods in Bihar, India
- Soil amendments reshape alpine meadow microbial communities across degradation gradients by modulating nutrient availability
- Light Rare Earth Elements in Plants: Environmental Sources, Bioavailability, Uptake, Nutrient Interactions, Mechanisms, and Concentration-Dependent Physiological and Signaling Reactions
- DTH1-dependent remodeling of the lipid and protein environment of lipid droplets during nutrient recovery in Chlamydomonas
BibTeX
@misc{almaas2026competing,
title = {{Competing constraints on protein availability and nutrient uptake reshape yeast genetic interactions}},
author = {E. Almaas and T. Kumelj},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.11.750905},
url = {https://www.biorxiv.org/content/10.64898/2026.09.11.750905v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Competing constraints on protein availability and nutrient uptake reshape yeast genetic interactions
AU - E. Almaas
AU - T. Kumelj
PY - 2026
DA - 2026/09/11
PB - bioRxiv
DO - 10.64898/2026.09.11.750905
UR - https://www.biorxiv.org/content/10.64898/2026.09.11.750905v1
AB - Epistasis predicted by enzyme-constrained metabolic models is computed at a single value of the protein available to metabolism, although that amount varies two- to three-fold with growth rate. We ask how epistatic interactions are affected by computing every single and pairwise gene deletion in an enzyme-constrained model of Saccharomyces cerevisiae while sweeping protein availability, with and without a bound on nutrient uptake. Protein availability changes relative fitness only under co-limitation, when the protein pool and a nutrient constraint limit growth together. The pool alone, the configuration in which these models are calibrated, makes protein a mere scale factor: no interaction changes sign. Under co-limitation, one genetic interaction in three is gained or lost across the physiological range. Most of this is inherited from single mutants of the respiratory machinery, which is recruited as protein becomes available. The epistasis between a glycolytic lesion with a protein-expensive bypass and the respiratory chain changes sign where the optimum switches from fermentation to respiration. We also find protein-priced interactions: the network supplies a bypass around each deletion and the pool gives it a price, so two genes with no stoichiometric coupling interact whenever the bypass around one competes with the route of the other for the protein pool. Isozyme pairs predicted neutral by flux balance analysis show negative epistasis for the same reason. Relative fitness depends on protein availability and nutrient supply only through their ratio. Thus, nutrient supply is a directly accessible experimental parameter to test epistatic interactions. Sign inversion, isozyme pairs, and interactions with cofactor-consuming branches are predictions a condition-resolved genetic interaction screen can test.
ER -