A coordinated transcriptional program controls de novo Golgi biogenesis

Abstract

The Golgi apparatus expands during differentiation and increased secretory demand, yet the transcriptional mechanisms governing its biogenesis remain poorly defined. To investigate this process, we developed an enzymatic approach to ablate preexisting Golgi and induce large-scale de novo organelle formation. Transcriptional profiling of cells rebuilding the Golgi revealed a coordinated induction of a broad gene network, coinciding with structural and functional maturation of the organelle. This network spans all Golgi subcompartments and activities, supporting the existence of a unified “Golgi regulon” that synchronizes expression of components required for organelle integrity. Failure to activate this regulon impaired Golgi reassembly. Promoter analysis and RNAi screening identified CREB3L1 as a central transcription factor for Golgi gene activation. Following Golgi loss, CREB3L1 translocates to the nucleus and activates its target genes, thereby driving biosynthesis of the components that are essential for organelle assembly. These findings demonstrate that Golgi biogenesis is governed by a coordinated transcriptional program that promotes organelle regeneration and enables secretory-pathway plasticity. This mechanism may support physiological remodeling by coupling Golgi biogenesis to cellular demand.

RubisCO-encoding methanotrophs elicit a coordinated transcriptional response to carbon dioxide limitation.
S. A. Lee, J. M. Henard, Y.-C. Yu +1
bioRxiv3 Nov 2026PreprintAll rights reservedFull textMicrobiologybioRxiv
A short C-terminal plug motif in a conserved type 4 pilus component controls pilus-tip localisation and filament biogenesis
M. Denic, M. Wuckelt, V. Roig-Zamboni +6
bioRxiv18 Sep 2026PreprintCC BYFull textMicrobiologybioRxiv
Transcriptional coupling of metabolism and cell mechanics controls human neuroepithelium morphogenesis
Camil Mirdass, Lucas Denis, Hugo Lachuer +19
HAL22 Sep 2026PreprintCC BY-NC-NDFull textDevelopmental biologyHALbioRxiv
Human macrophages activate a coordinated program linking inflammation and cell death in response to intracellular Poly(I:C)
Luigi Fiore, Lara Di Leonardo, Marco Buscetta +11
Frontiers in immunology2 Oct 2026Peer-reviewedCC BYFull textImmunologyEurope PMCCrossref
Apelin signaling controls neuroendocrine homeostasis via Atoh8-dependent transcriptional regulation
R. Menard, A. Neog, C. Halluin +2
bioRxiv2 Nov 2026PreprintCC BY-NC-NDFull textDevelopmental biologybioRxiv

Cite

BibTeX RIS

BibTeX

@article{forno2026coordinated,
  title = {{A coordinated transcriptional program controls de novo Golgi biogenesis}},
  author = {Francesca Forno and Domenico Abete and Elena Polishchuk and Xabier Bujanda Cundin and Fioranna Renda and Roberta Crispino and Josephine Salzano and Raffaella Petruzzelli and Rossella de Cegli and Martina Sofia and Nicolina Cristina Sorrentino and Lorenzo Vaccaro and Davide Cacchiarelli and Juul Verbakel and Jan de Boer and Bruno Goud and Alexey Khodjakov and Franck Perez and Roman Polishchuk},
  journal = {The EMBO Journal},
  year = {2026},
  month = jun,
  doi = {10.1038/s44318-026-00828-7},
  url = {https://hal.science/hal-05780994v1},
  note = {CC BY-SA 4.0},
}

RIS

TY  - JOUR
TI  - A coordinated transcriptional program controls de novo Golgi biogenesis
AU  - Francesca Forno
AU  - Domenico Abete
AU  - Elena Polishchuk
AU  - Xabier Bujanda Cundin
AU  - Fioranna Renda
AU  - Roberta Crispino
AU  - Josephine Salzano
AU  - Raffaella Petruzzelli
AU  - Rossella de Cegli
AU  - Martina Sofia
AU  - Nicolina Cristina Sorrentino
AU  - Lorenzo Vaccaro
AU  - Davide Cacchiarelli
AU  - Juul Verbakel
AU  - Jan de Boer
AU  - Bruno Goud
AU  - Alexey Khodjakov
AU  - Franck Perez
AU  - Roman Polishchuk
PY  - 2026
DA  - 2026/06/06
JO  - The EMBO Journal
DO  - 10.1038/s44318-026-00828-7
UR  - https://hal.science/hal-05780994v1
AB  - The Golgi apparatus expands during differentiation and increased secretory demand, yet the transcriptional mechanisms governing its biogenesis remain poorly defined. To investigate this process, we developed an enzymatic approach to ablate preexisting Golgi and induce large-scale de novo organelle formation. Transcriptional profiling of cells rebuilding the Golgi revealed a coordinated induction of a broad gene network, coinciding with structural and functional maturation of the organelle. This network spans all Golgi subcompartments and activities, supporting the existence of a unified “Golgi regulon” that synchronizes expression of components required for organelle integrity. Failure to activate this regulon impaired Golgi reassembly. Promoter analysis and RNAi screening identified CREB3L1 as a central transcription factor for Golgi gene activation. Following Golgi loss, CREB3L1 translocates to the nucleus and activates its target genes, thereby driving biosynthesis of the components that are essential for organelle assembly. These findings demonstrate that Golgi biogenesis is governed by a coordinated transcriptional program that promotes organelle regeneration and enables secretory-pathway plasticity. This mechanism may support physiological remodeling by coupling Golgi biogenesis to cellular demand.
SN  - 0261-4189
SN  - 1460-2075
ER  -

Abstract

Related

Cite