Mangiferin inhibits multiple stages of the Toxoplasma gondii lytic cycle in vitro and protects against acute…

Abstract

Introduction: Toxoplasmosis remains an important global health concern, highlighting the need for novel agents anti- Toxoplasma gondii ( T. gondii ). Mangiferin, derived from Mangifera indica , has demonstrated diverse pharmacological and antiprotozoal activities, but its effects against T. gondii remain unclear.

Methods: We evaluated the anti- T. gondii activity of mangiferin in vitro and in vivo . Cytotoxicity toward human foreskin fibroblast (HFF) cells was assessed, followed by evaluation of parasite plaque formation and extracellular tachyzoite morphology. Its protective effects were further investigated in a murine model of acute RH strain infection, and its effects on parasite adhesion, invasion, and intracellular proliferation were examined.

Results: Mangiferin showed low cytotoxicity toward HFF cells, with cell viability remaining above 90% at concentrations up to 320 μM. Mangiferin markedly inhibited parasite plaque formation and induced rapid morphological changes in extracellular tachyzoites. In a murine model of acute RH strain infection, mangiferin prolonged survival, alleviated splenic pathological damage, and reduced the relative parasite burden in the blood, spleen, and brain. Stage specific analyses further demonstrated that mangiferin inhibited parasite adhesion, invasion, and intracellular proliferation.

Conclusion: Mangiferin exhibits anti- T. gondii activity both in vitro and in vivo and affects multiple processes within the T. gondii lytic cycle. These findings provide initial experimental evidence supporting further investigation of mangiferin as a potential natural compound for the development of novel strategies against toxoplasmosis.

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BibTeX RIS

BibTeX

@article{gao2026mangiferin,
  title = {{Mangiferin inhibits multiple stages of the Toxoplasma gondii lytic cycle in vitro and protects against acute RH strain infection in vivo}},
  author = {Shuoxiang Gao and Yingzhuo Dou and Mengyi Li and Junru Wu and Tingting Ying and Zhenchao Zhang and Zhenke Yang and Xuefang Mei and Shuai Wang and Xiaowei Tian},
  journal = {Frontiers in Microbiology},
  year = {2026},
  month = oct,
  doi = {10.3389/fmicb.2026.1983878},
  url = {https://www.frontiersin.org/articles/10.3389/fmicb.2026.1983878/full},
  note = {CC BY 4.0},
}

RIS

TY  - JOUR
TI  - Mangiferin inhibits multiple stages of the Toxoplasma gondii lytic cycle in vitro and protects against acute RH strain infection in vivo
AU  - Shuoxiang Gao
AU  - Yingzhuo Dou
AU  - Mengyi Li
AU  - Junru Wu
AU  - Tingting Ying
AU  - Zhenchao Zhang
AU  - Zhenke Yang
AU  - Xuefang Mei
AU  - Shuai Wang
AU  - Xiaowei Tian
PY  - 2026
DA  - 2026/10/23
JO  - Frontiers in Microbiology
DO  - 10.3389/fmicb.2026.1983878
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2026.1983878/full
AB  - Introduction: Toxoplasmosis remains an important global health concern, highlighting the need for novel agents anti- Toxoplasma gondii ( T. gondii ). Mangiferin, derived from Mangifera indica , has demonstrated diverse pharmacological and antiprotozoal activities, but its effects against T. gondii remain unclear.  Methods: We evaluated the anti- T. gondii activity of mangiferin in vitro and in vivo . Cytotoxicity toward human foreskin fibroblast (HFF) cells was assessed, followed by evaluation of parasite plaque formation and extracellular tachyzoite morphology. Its protective effects were further investigated in a murine model of acute RH strain infection, and its effects on parasite adhesion, invasion, and intracellular proliferation were examined.  Results: Mangiferin showed low cytotoxicity toward HFF cells, with cell viability remaining above 90% at concentrations up to 320 μM. Mangiferin markedly inhibited parasite plaque formation and induced rapid morphological changes in extracellular tachyzoites. In a murine model of acute RH strain infection, mangiferin prolonged survival, alleviated splenic pathological damage, and reduced the relative parasite burden in the blood, spleen, and brain. Stage specific analyses further demonstrated that mangiferin inhibited parasite adhesion, invasion, and intracellular proliferation.  Conclusion: Mangiferin exhibits anti- T. gondii activity both in vitro and in vivo and affects multiple processes within the T. gondii lytic cycle. These findings provide initial experimental evidence supporting further investigation of mangiferin as a potential natural compound for the development of novel strategies against toxoplasmosis.
SN  - 1664-302X
ER  -

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