Comparative evaluation of a novel strontium silicate-based sealer on dental stem cells from human: an in vitro study.
4 affiliations
- 1South China Center of Craniofacial Stem Cell Research and Guangdong Province Key Laboratory of Stomatology, Guanghua School and Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, 510055, People's Republic of China.
- 2Department of periodontology, Guanghua School and Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, 510055, People's Republic of China.
- 3South China Center of Craniofacial Stem Cell Research and Guangdong Province Key Laboratory of Stomatology, Guanghua School and Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, 510055, People's Republic of China. [email protected].
- 4South China Center of Craniofacial Stem Cell Research and Guangdong Province Key Laboratory of Stomatology, Guanghua School and Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, 510055, People's Republic of China. [email protected].
Abstract
Background
This study aimed to evaluate and compare the biological effects of a novel strontium silicate-based bio-ceramic sealer (CRoot SP) with two widely used sealers, AH Plus (epoxy resin-based) and iRoot SP (calcium silicate-based), on human dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs).Methods
In vitro assays were conducted to assess cytotoxicity (using Cell Counting Kit-8 reagent, CCK-8), migration (wound healing assay), osteogenic differentiation (alizarin red S staining and western blot analysis), anti-inflammatory properties (reactive oxygen species (ROS) and nitric oxide (NO) detection), and antibacterial efficacy against Enterococcus faecalis (E. faecalis). Sealer extracts were prepared at concentrations ranging from 0.02 to 10 mg/mL in accordance with ISO 10993-5.Results
CRoot SP exhibited excellent biocompatibility and significantly enhanced cell proliferation, particularly in DPSCs at higher concentrations. It markedly promoted PDLSCs migration and osteogenic differentiation at low concentrations (0.02-0.2 mg/mL), though this effect decreased with increasing concentration. CRoot SP also demonstrated the highest antibacterial activity against E. faecalis and the lowest levels of ROS and NO, indicating strong anti-inflammatory properties. iRoot SP exhibited favorable bioactivity and concentration-dependent promotion of osteogenesis but induced higher levels of inflammatory mediators. AH Plus exhibited significant cytotoxicity, especially at high concentrations, inhibiting cell proliferation and migration, likely due to the release of toxic monomers.Conclusion
CRoot SP possesses superior biocompatibility, pro-regenerative properties at low concentrations, anti-inflammatory potential, and antibacterial efficacy compared to iRoot SP and AH Plus, supporting its clinical potential for use in regenerative endodontic procedures.
Related
- Comparative evaluation on the bond strength of AH PLUS sealer to root dentin after using different gutta-percha solvents during retreatment
- Modulating the Physicochemical Properties of Mesoporous Strontium Silicate Nanostructures Using a Design-of-Experiments Approach
- Effect of delivery method on periapical extrusion of a hydraulic calcium silicate cement-based sealer during single-cone root canal filling technique: An in vitro study.
- Intracanal adaptation and bond strength assessment of novel liquid/powder calcium silicate-based root canal sealer.
- Effect of Residual Glass Fraction on the Crystallization Behavior and Thermomechanical Properties of STS (Strontium Titanium Silicate) Glass-Ceramics
BibTeX
@article{sun2026comparative,
title = {{Comparative evaluation of a novel strontium silicate-based sealer on dental stem cells from human: an in vitro study.}},
author = {Xiaohan Sun and Wenwen Li and Panpan Wang and Songtao Shi and Xueli Mao},
journal = {BMC oral health},
year = {2026},
month = jul,
doi = {10.1186/s12903-026-09139-z},
url = {https://europepmc.org/article/MED/42958539},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Comparative evaluation of a novel strontium silicate-based sealer on dental stem cells from human: an in vitro study.
AU - Xiaohan Sun
AU - Wenwen Li
AU - Panpan Wang
AU - Songtao Shi
AU - Xueli Mao
PY - 2026
DA - 2026/07/23
JO - BMC oral health
DO - 10.1186/s12903-026-09139-z
UR - https://europepmc.org/article/MED/42958539
AB - Background This study aimed to evaluate and compare the biological effects of a novel strontium silicate-based bio-ceramic sealer (CRoot SP) with two widely used sealers, AH Plus (epoxy resin-based) and iRoot SP (calcium silicate-based), on human dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs).Methods In vitro assays were conducted to assess cytotoxicity (using Cell Counting Kit-8 reagent, CCK-8), migration (wound healing assay), osteogenic differentiation (alizarin red S staining and western blot analysis), anti-inflammatory properties (reactive oxygen species (ROS) and nitric oxide (NO) detection), and antibacterial efficacy against Enterococcus faecalis (E. faecalis). Sealer extracts were prepared at concentrations ranging from 0.02 to 10 mg/mL in accordance with ISO 10993-5.Results CRoot SP exhibited excellent biocompatibility and significantly enhanced cell proliferation, particularly in DPSCs at higher concentrations. It markedly promoted PDLSCs migration and osteogenic differentiation at low concentrations (0.02-0.2 mg/mL), though this effect decreased with increasing concentration. CRoot SP also demonstrated the highest antibacterial activity against E. faecalis and the lowest levels of ROS and NO, indicating strong anti-inflammatory properties. iRoot SP exhibited favorable bioactivity and concentration-dependent promotion of osteogenesis but induced higher levels of inflammatory mediators. AH Plus exhibited significant cytotoxicity, especially at high concentrations, inhibiting cell proliferation and migration, likely due to the release of toxic monomers.Conclusion CRoot SP possesses superior biocompatibility, pro-regenerative properties at low concentrations, anti-inflammatory potential, and antibacterial efficacy compared to iRoot SP and AH Plus, supporting its clinical potential for use in regenerative endodontic procedures.
SN - 1472-6831
ER -