3D-Printable and Cytocompatible Hydrogel from Acinetobacter baylyi ADP1 Extracellular Matrix

Abstract

Tissue engineering has advanced significantly, yet multicomponent hydrogels inspired by the compositional complexity of natural extracellular matrices (ECMs) are still underexplored. Most current hydrogels are based on single-component formulations, which can limit their biochemical and mechanical versatility. Developing synthetic multicomponent hydrogels remains challenging because it requires the controlled integration of multiple functional groups within a single material platform. Here, a biologically driven strategy is introduced by leveraging Acinetobacter baylyi ADP1, a bacterium that naturally produces extracellular polymeric substances (EPS) composed of a multicomponent matrix of polysaccharides and proteins. Through three-day cultivation and a simple extraction method, a hydrogel is obtained that can be methacrylated and photocrosslinked using red or blue light. This hydrogel is porous, cytocompatible, 3D-bioprintable, injectable, and undergoes rapid gelation for in situ crosslinking. This work highlights the potential of using bacterial-derived multicomponent hydrogels for biofabrication.

Phage cocktail-loaded hydrogel coating prevents the biofilm formation of MDR Acinetobacter baumannii on indwelling medical devices
Nisha K. Rai, Kokkarambath Vannadil Suchithra, Juliet Roshini Mohan Raj +3
Frontiers in Microbiology30 Oct 2026Peer-reviewedCC BY 4.0MicrobiologyFrontiersCrossref
M2 Macrophage‐Derived Small Extracellular Vesicle miR‐106a‐Loaded QCG Hydrogel Targets the RNF128‐LXR Pathway to Reprogram Chondrocyte Cholesterol Metabolism for Osteoarthritis Therapy
Baozhe Liu, Zhan Wang, Jingyong Zhang +3
Journal of extracellular vesicles1 Oct 2026Peer-reviewedCC BY-NCFull textMolecular biology & gene regulation Cell biologyEurope PMCDOAJ
GelMA hydrogel enhances diabetic wound healing by delivering shock wave-preconditioned BMSC-derived small extracellular vesicles
Rongqi Zhou, Xiaoping Xie, Xudong Li +5
Frontiers in Immunology28 Oct 2026Peer-reviewedCC BY 4.0ImmunologyCrossref
An open-source, 3D-printable droplet microfluidics platform with strobe-enhanced microscopy
P. Padilla-Huamantinco, M. Hurtado-Labarca, C. Nel +5
bioRxiv19 Oct 2026PreprintCC BYFull textSystems & synthetic biologybioRxiv
Metabolic acidification–linked outer membrane vesiculation in Acinetobacter baumannii
Jihyeon Min, Yerim Park, Jihye Bae +2
Communications Biology1 Oct 2026Peer-reviewedCC BY-NC-ND 4.0Full textGenetics & genomicsCrossref

Cite

BibTeX RIS

BibTeX

@misc{radmehr20263dprintable,
  title = {{3D-Printable and Cytocompatible Hydrogel from Acinetobacter baylyi ADP1 Extracellular Matrix}},
  author = {S. Radmehr and P. C. Ingoni and A. Eftekhari and L. Ylä-Outinen and V. S. Parihar and M. Khavani and N. Perho and J. Morikka and D. Greco and T. Laaksonen and M. Kellomäki and H. Skottman and S. Santala and V. Santala},
  howpublished = {bioRxiv},
  year = {2026},
  month = sep,
  doi = {10.64898/2026.09.11.750904},
  url = {https://www.biorxiv.org/content/10.64898/2026.09.11.750904v1},
  note = {CC BY-ND},
}

RIS

TY  - UNPB
TI  - 3D-Printable and Cytocompatible Hydrogel from Acinetobacter baylyi ADP1 Extracellular Matrix
AU  - S. Radmehr
AU  - P. C. Ingoni
AU  - A. Eftekhari
AU  - L. Ylä-Outinen
AU  - V. S. Parihar
AU  - M. Khavani
AU  - N. Perho
AU  - J. Morikka
AU  - D. Greco
AU  - T. Laaksonen
AU  - M. Kellomäki
AU  - H. Skottman
AU  - S. Santala
AU  - V. Santala
PY  - 2026
DA  - 2026/09/11
PB  - bioRxiv
DO  - 10.64898/2026.09.11.750904
UR  - https://www.biorxiv.org/content/10.64898/2026.09.11.750904v1
AB  - Tissue engineering has advanced significantly, yet multicomponent hydrogels inspired by the compositional complexity of natural extracellular matrices (ECMs) are still underexplored. Most current hydrogels are based on single-component formulations, which can limit their biochemical and mechanical versatility. Developing synthetic multicomponent hydrogels remains challenging because it requires the controlled integration of multiple functional groups within a single material platform. Here, a biologically driven strategy is introduced by leveraging Acinetobacter baylyi ADP1, a bacterium that naturally produces extracellular polymeric substances (EPS) composed of a multicomponent matrix of polysaccharides and proteins. Through three-day cultivation and a simple extraction method, a hydrogel is obtained that can be methacrylated and photocrosslinked using red or blue light. This hydrogel is porous, cytocompatible, 3D-bioprintable, injectable, and undergoes rapid gelation for in situ crosslinking. This work highlights the potential of using bacterial-derived multicomponent hydrogels for biofabrication.
ER  -

Abstract

Related

Cite