Differential Exon Usage in Expression Profiles from Stress Axis Tissues of Brahman Cows Subjected to Prenatal Stress.
5 affiliations
- 1Department of Animal Science, Texas A&M University, College Station, TX 77843, USA.
- 2Department of Agricultural Sciences, West Texas A&M University, Canyon, TX 79016, USA.
- 3Texas A&M AgriLife Research & Extension Center at Overton, Overton, TX 75684, USA.
- 4Department of Computer Science, Prairie View A&M University, Prairie View, TX 77446, USA.
- 5Texas A&M AgriLife Research, College Station, TX 77843, USA.
Abstract
Background/objectives
Alteration in gene expression caused by stress may serve as a potential mechanism behind a range of differences previously observed in progeny exposed to prenatal stress. The objective of this study was to examine the impact of prenatal transportation stress on differential exon usage in expressed genes of stress-axis tissues of control and prenatally stressed Brahman cattle at five years of age.Methods
Pregnant Brahman cows were transported for 2 h sessions at 20-day intervals from the 60th to the 140th day of gestation, while pregnant herdmates served as controls. Blood samples were collected from six prenatally stressed (PNS) and eight control (control) females obtained when they were 5 years of age. Tissue samples were harvested from the adrenal medulla, adrenal cortex, anterior pituitary and hypothalamic paraventricular nucleus of the PNS and control cows at 5 years of age. Differential exon usage was analyzed by the DEXSeq tool to identify genes that were not previously identified by RNA sequencing analyses.Results
Differential exon usage (FDR ≤ 0.05) was detected in three exons (two genes) in the adrenal medulla, six exons (five genes) in the adrenal cortex, 13 exons (12 genes) in the anterior pituitary, and 37 exons (33 genes) in the hypothalamic paraventricular nucleus.Conclusions
The correspondence of prenatal stress to changes in exon usage across multiple tissues and genes could indicate investigative opportunities in postnatally affected physiological processes, including myelination, brain development, stress responses and metabolism.
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BibTeX
@article{cilkiz2026differential,
title = {{Differential Exon Usage in Expression Profiles from Stress Axis Tissues of Brahman Cows Subjected to Prenatal Stress.}},
author = {Kubra Z Cilkiz and Emilie C Baker and Charles R Long and Ronald D Randel and Noushin Ghaffari and Thomas H Welsh and David G Riley},
journal = {Genes},
year = {2026},
month = sep,
doi = {10.3390/genes17101208},
url = {https://europepmc.org/article/MED/42938324},
note = {CC BY},
}
RIS
TY - JOUR
TI - Differential Exon Usage in Expression Profiles from Stress Axis Tissues of Brahman Cows Subjected to Prenatal Stress.
AU - Kubra Z Cilkiz
AU - Emilie C Baker
AU - Charles R Long
AU - Ronald D Randel
AU - Noushin Ghaffari
AU - Thomas H Welsh
AU - David G Riley
PY - 2026
DA - 2026/09/29
JO - Genes
DO - 10.3390/genes17101208
UR - https://europepmc.org/article/MED/42938324
AB - Background/objectives Alteration in gene expression caused by stress may serve as a potential mechanism behind a range of differences previously observed in progeny exposed to prenatal stress. The objective of this study was to examine the impact of prenatal transportation stress on differential exon usage in expressed genes of stress-axis tissues of control and prenatally stressed Brahman cattle at five years of age.Methods Pregnant Brahman cows were transported for 2 h sessions at 20-day intervals from the 60th to the 140th day of gestation, while pregnant herdmates served as controls. Blood samples were collected from six prenatally stressed (PNS) and eight control (control) females obtained when they were 5 years of age. Tissue samples were harvested from the adrenal medulla, adrenal cortex, anterior pituitary and hypothalamic paraventricular nucleus of the PNS and control cows at 5 years of age. Differential exon usage was analyzed by the DEXSeq tool to identify genes that were not previously identified by RNA sequencing analyses.Results Differential exon usage (FDR ≤ 0.05) was detected in three exons (two genes) in the adrenal medulla, six exons (five genes) in the adrenal cortex, 13 exons (12 genes) in the anterior pituitary, and 37 exons (33 genes) in the hypothalamic paraventricular nucleus.Conclusions The correspondence of prenatal stress to changes in exon usage across multiple tissues and genes could indicate investigative opportunities in postnatally affected physiological processes, including myelination, brain development, stress responses and metabolism.
SN - 2073-4425
ER -