GoAstV Induced Mitophagy and Ferroptosis in the Spleen of Goslings.
- 1Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
- 2Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China.
- 3School of Computer and Information Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
Abstract
A novel goose astrovirus (GoAstV) has emerged in various provinces of China, leading to a mortality rate of up to 50% among GoAstV goslings. The spleen is an important immune organ in geese. Studies have shown that goslings infected with GoAstV exhibit higher viral loads in their spleens, and the spleens sustain damage. However, the mechanism underlying its induction of spleen injury remains to be elucidated. Therefore, a gosling infection model with GoAstV was established in this experiment. The Con group was subcutaneously injected with 0.2 mL of saline, while the GoAstV group was subcutaneously injected with GoAstV JX01 (2 × 10⁶ TCID50/0.2 mL). Spleen tissues were collected on days 1, 3, 6, 9 post-infection (dpi). The results showed that the body weight of the GoAstV group decreased significantly, while the spleen weight and spleen index increased significantly. Post-mortem examination revealed that the spleen of the GoAstV group was significantly enlarged. The viral load in the spleen reached its peak at 6 dpi. Histopathological and electron microscopy revealed lymphocyte necrosis, autophagic lysosomes, and mitochondria autophagosomes. The spleen of the GoAstV group exhibited increased iron content, elevated malondialdehyde (MDA) production, decreased glutathione (GSH) content, and reduced glutathione peroxidase (GSH-Px) activity. RT-qPCR and Western blot analyses revealed that GoAstV upregulated mRNA expression of pro-inflammatory cytokines and inhibited anti-inflammatory cytokines. GoAstV also upregulated genes and proteins linked to ferroptosis and mitophagy, while downregulating those associated with anti-ferroptosis and SQSTM1. In conclusion, our findings suggest that GoAstV infection may induce mitophagy and ferroptosis in the gosling spleen, thereby contributing to splenic injury.
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BibTeX
@article{chen2026goastv,
title = {{GoAstV Induced Mitophagy and Ferroptosis in the Spleen of Goslings.}},
author = {Yunfeng Chen and Duanfeng Fu and Haiqin Li and Yan Shi and Zhihua Lu and Haoyu Huang and Xiaona Gao and Xiaoquan Guo},
journal = {Animals : an open access journal from MDPI},
year = {2026},
month = sep,
doi = {10.3390/ani16193019},
url = {https://europepmc.org/article/MED/42882988},
note = {CC BY},
}
RIS
TY - JOUR
TI - GoAstV Induced Mitophagy and Ferroptosis in the Spleen of Goslings.
AU - Yunfeng Chen
AU - Duanfeng Fu
AU - Haiqin Li
AU - Yan Shi
AU - Zhihua Lu
AU - Haoyu Huang
AU - Xiaona Gao
AU - Xiaoquan Guo
PY - 2026
DA - 2026/09/25
JO - Animals : an open access journal from MDPI
DO - 10.3390/ani16193019
UR - https://europepmc.org/article/MED/42882988
AB - A novel goose astrovirus (GoAstV) has emerged in various provinces of China, leading to a mortality rate of up to 50% among GoAstV goslings. The spleen is an important immune organ in geese. Studies have shown that goslings infected with GoAstV exhibit higher viral loads in their spleens, and the spleens sustain damage. However, the mechanism underlying its induction of spleen injury remains to be elucidated. Therefore, a gosling infection model with GoAstV was established in this experiment. The Con group was subcutaneously injected with 0.2 mL of saline, while the GoAstV group was subcutaneously injected with GoAstV JX01 (2 × 10⁶ TCID50/0.2 mL). Spleen tissues were collected on days 1, 3, 6, 9 post-infection (dpi). The results showed that the body weight of the GoAstV group decreased significantly, while the spleen weight and spleen index increased significantly. Post-mortem examination revealed that the spleen of the GoAstV group was significantly enlarged. The viral load in the spleen reached its peak at 6 dpi. Histopathological and electron microscopy revealed lymphocyte necrosis, autophagic lysosomes, and mitochondria autophagosomes. The spleen of the GoAstV group exhibited increased iron content, elevated malondialdehyde (MDA) production, decreased glutathione (GSH) content, and reduced glutathione peroxidase (GSH-Px) activity. RT-qPCR and Western blot analyses revealed that GoAstV upregulated mRNA expression of pro-inflammatory cytokines and inhibited anti-inflammatory cytokines. GoAstV also upregulated genes and proteins linked to ferroptosis and mitophagy, while downregulating those associated with anti-ferroptosis and SQSTM1. In conclusion, our findings suggest that GoAstV infection may induce mitophagy and ferroptosis in the gosling spleen, thereby contributing to splenic injury.
SN - 2076-2615
ER -