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Peer-reviewedCC BYInternational journal of molecular sciences

A Colorimetric Approach to Identifying Single-Nucleotide Mutations Using Gold-Based Magnetic Nanoparticles

  • Anna Epanchintseva1
  • Julia Shyiskaya1
  • Julia Poletaeva1
  • Ekaterina Baeva1
  • Evgenia Kozyreva1
  • Evgeniya Dyudeyeva1
  • Mariya Khokhlova2
  • Inna Pyshnaya1
  1. 1Knorre Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia
  2. 2Institute of Solid State Chemistry and Mechanochemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia
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Abstract

Detection of single-nucleotide mutations (SNPs) in the genome of infectious agents is one of the ways to identify their drug-resistant strains. Antibiotic-resistant strains of Mycobacterium tuberculosis (MTB) impede advances in the fight against the spread of tuberculosis. Diagnostic tools exist for both MTB itself and its drug-resistant strains, most of which are technically complex, resource-intensive, and time-consuming. This paper presents the development of a colorimetric test system, requiring no expensive equipment, for the rapid and accurate determination of the SNPs associated with drug-resistant forms of MTB to fluoroquinolones in modeled DNA targets (198 bp). For this purpose, nanoconstructs based on gold nanoparticles (AuNPs) and hybrid magnetic Fe3O4@Au NPs were created, leveraging the advantages of each nanomaterial. The use of AuNPs provides a basis for colorimetric detection due to their high optical absorption. Fe3O4@Au NPs enable samples to respond to an external magnetic field. The study demonstrates the ability to reliably discriminate between wild-type and mutant-model DNA targets in the nanomolar concentration range within 15 min. Visual analysis and optical absorption spectroscopy were used to evaluate the detection results. The application of an external magnetic field allows for enhanced colorimetric distinction between wild-type target samples and those containing an SNP.

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Cite

BibTeXRIS
BibTeX
@article{epanchintseva2026colorimetric,
  title = {{A Colorimetric Approach to Identifying Single-Nucleotide Mutations Using Gold-Based Magnetic Nanoparticles}},
  author = {Anna Epanchintseva and Julia Shyiskaya and Julia Poletaeva and Ekaterina Baeva and Evgenia Kozyreva and Evgeniya Dyudeyeva and Mariya Khokhlova and Inna Pyshnaya},
  journal = {International journal of molecular sciences},
  year = {2026},
  month = oct,
  doi = {10.3390/ijms27198809},
  url = {https://europepmc.org/article/PMC/PMC13665408},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - A Colorimetric Approach to Identifying Single-Nucleotide Mutations Using Gold-Based Magnetic Nanoparticles
AU  - Anna Epanchintseva
AU  - Julia Shyiskaya
AU  - Julia Poletaeva
AU  - Ekaterina Baeva
AU  - Evgenia Kozyreva
AU  - Evgeniya Dyudeyeva
AU  - Mariya Khokhlova
AU  - Inna Pyshnaya
PY  - 2026
DA  - 2026/10/01
JO  - International journal of molecular sciences
DO  - 10.3390/ijms27198809
UR  - https://europepmc.org/article/PMC/PMC13665408
AB  - Detection of single-nucleotide mutations (SNPs) in the genome of infectious agents is one of the ways to identify their drug-resistant strains. Antibiotic-resistant strains of Mycobacterium tuberculosis (MTB) impede advances in the fight against the spread of tuberculosis. Diagnostic tools exist for both MTB itself and its drug-resistant strains, most of which are technically complex, resource-intensive, and time-consuming. This paper presents the development of a colorimetric test system, requiring no expensive equipment, for the rapid and accurate determination of the SNPs associated with drug-resistant forms of MTB to fluoroquinolones in modeled DNA targets (198 bp). For this purpose, nanoconstructs based on gold nanoparticles (AuNPs) and hybrid magnetic Fe3O4@Au NPs were created, leveraging the advantages of each nanomaterial. The use of AuNPs provides a basis for colorimetric detection due to their high optical absorption. Fe3O4@Au NPs enable samples to respond to an external magnetic field. The study demonstrates the ability to reliably discriminate between wild-type and mutant-model DNA targets in the nanomolar concentration range within 15 min. Visual analysis and optical absorption spectroscopy were used to evaluate the detection results. The application of an external magnetic field allows for enhanced colorimetric distinction between wild-type target samples and those containing an SNP.
SN  - 1422-0067
ER  -