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Peer-reviewedCC BYChemical Science

Archaerhodopsin 3 is an ideal template for the engineering of highly fluorescent optogenetic reporters

  • Krystyna Herasymenko
  • Danushka Walisinghe
  • Masae Konno
  • Leonardo Barneschi
  • Isabelle de Waele
  • Michel Sliwa
  • Keiichi Inoue
  • Massimo Olivucci
  • Stefan Haacke
Open at sourceDOICite

Abstract

retinal chromophore leading to a metastable twisted diradical (TIDIR), in clear contrast to the standard charge-transfer scenario established for other microbial rhodopsins. We discuss how the presence of TIDIR makes AR-3 an ideal candidate for the design of variants with a one-photon induced fluorescence possibly reaching the emission quantum yield of the top natural emitter neorhodopsin (NeoR).

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Cite

BibTeXRIS
BibTeX
@article{herasymenko2026archaerhodopsin,
  title = {{Archaerhodopsin 3 is an ideal template for the engineering of highly fluorescent optogenetic reporters}},
  author = {Krystyna Herasymenko and Danushka Walisinghe and Masae Konno and Leonardo Barneschi and Isabelle de Waele and Michel Sliwa and Keiichi Inoue and Massimo Olivucci and Stefan Haacke},
  journal = {Chemical Science},
  year = {2026},
  month = oct,
  doi = {10.1039/d4sc05120c},
  url = {https://hal.science/hal-05780454v1},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Archaerhodopsin 3 is an ideal template for the engineering of highly fluorescent optogenetic reporters
AU  - Krystyna Herasymenko
AU  - Danushka Walisinghe
AU  - Masae Konno
AU  - Leonardo Barneschi
AU  - Isabelle de Waele
AU  - Michel Sliwa
AU  - Keiichi Inoue
AU  - Massimo Olivucci
AU  - Stefan Haacke
PY  - 2026
DA  - 2026/10/06
JO  - Chemical Science
DO  - 10.1039/d4sc05120c
UR  - https://hal.science/hal-05780454v1
AB  - retinal chromophore leading to a metastable twisted diradical (TIDIR), in clear contrast to the standard charge-transfer scenario established for other microbial rhodopsins. We discuss how the presence of TIDIR makes AR-3 an ideal candidate for the design of variants with a one-photon induced fluorescence possibly reaching the emission quantum yield of the top natural emitter neorhodopsin (NeoR).
SN  - 2041-6520
SN  - 2041-6539
ER  -