A Novel Boranil-Based Colorimetric and Fluorescence Quenching Probe for Selective Detection and Bioimaging of the Fe3+ ion
4 affiliations
- 1Kamla Nehru Institute of Physical and Social Sciences , , ,
- 2Guru Ghasidas Vishwavidyalaya , , , ,
- 3Rajkiya Engineering College , , ,
- 4Banaras Hindu University , , ,
Abstract
A novel, readily synthesizable boranil-based chemosensor, HSR, was structurally and spectroscopically characterized by single-crystal X-ray diffraction, IR spectroscopy, 1H, 13C, and 11B NMR spectroscopy, and high-resolution mass spectrometry (HRMS). HSR exhibited highly selective colorimetric and fluorescence responses toward the Fe3+ ion over a wide range of competing metal ions, including Na+, K+, Ba2+, Ca2+, Mg2+, Al3+, Zn2+, Ni2+, Cu2+, Co3+, Pb2+, Cd2+, Hg2+, and Fe2+. Upon Fe3+ addition, the solution color changes from pale yellow to red-brown, accompanied by pronounced quenching of fluorescence signals. Concentration-dependent Fe3+ ion titrations revealed significant modulation of the photoluminescence and UV–vis absorption profiles. The binding stoichiometry and association constant between HSR and the Fe3+ ion were determined by UV–vis and fluorescence titrations, Job’s plot analysis, and HRMS, confirming strong complex formation. Notably, no interference from biologically relevant or toxic metal ions was observed. The limit of detection (LOD), calculated using the 3σ method, was 7.5 × 10–6 M. The association constant (Ka), obtained from a Benesi–Hildebrand analysis, was 2.35 × 1015 M–1, indicating exceptionally strong Fe3+ ion binding. Furthermore, HSR displayed good solubility in mixed EtOH/H2O media and efficient cellular uptake with no cytotoxicity, highlighting its suitability for the detection of the Fe3+ ion and bioimaging applications.
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BibTeX
@article{parveen2026novel,
title = {{A Novel Boranil-Based Colorimetric and Fluorescence Quenching Probe for Selective Detection and Bioimaging of the Fe3+ ion}},
author = {Darakhshan Parveen and Saumya Singh and Avadhesh Pratap Singh and Alok Kumar Verma and Abhishek Kumar and Chandni Singh and Suryabhan Singh and Ashish Kumar Singh and Divya Pratap Singh and Mukesh Kumar and Ajay Kumar},
journal = {ACS omega},
year = {2026},
month = sep,
doi = {10.1021/acsomega.6c01069},
url = {https://europepmc.org/article/PMC/PMC13670870},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - A Novel Boranil-Based Colorimetric and Fluorescence Quenching Probe for Selective Detection and Bioimaging of the Fe3+ ion
AU - Darakhshan Parveen
AU - Saumya Singh
AU - Avadhesh Pratap Singh
AU - Alok Kumar Verma
AU - Abhishek Kumar
AU - Chandni Singh
AU - Suryabhan Singh
AU - Ashish Kumar Singh
AU - Divya Pratap Singh
AU - Mukesh Kumar
AU - Ajay Kumar
PY - 2026
DA - 2026/09/30
JO - ACS omega
DO - 10.1021/acsomega.6c01069
UR - https://europepmc.org/article/PMC/PMC13670870
AB - A novel, readily synthesizable boranil-based chemosensor, HSR, was structurally and spectroscopically characterized by single-crystal X-ray diffraction, IR spectroscopy, 1H, 13C, and 11B NMR spectroscopy, and high-resolution mass spectrometry (HRMS). HSR exhibited highly selective colorimetric and fluorescence responses toward the Fe3+ ion over a wide range of competing metal ions, including Na+, K+, Ba2+, Ca2+, Mg2+, Al3+, Zn2+, Ni2+, Cu2+, Co3+, Pb2+, Cd2+, Hg2+, and Fe2+. Upon Fe3+ addition, the solution color changes from pale yellow to red-brown, accompanied by pronounced quenching of fluorescence signals. Concentration-dependent Fe3+ ion titrations revealed significant modulation of the photoluminescence and UV–vis absorption profiles. The binding stoichiometry and association constant between HSR and the Fe3+ ion were determined by UV–vis and fluorescence titrations, Job’s plot analysis, and HRMS, confirming strong complex formation. Notably, no interference from biologically relevant or toxic metal ions was observed. The limit of detection (LOD), calculated using the 3σ method, was 7.5 × 10–6 M. The association constant (Ka), obtained from a Benesi–Hildebrand analysis, was 2.35 × 1015 M–1, indicating exceptionally strong Fe3+ ion binding. Furthermore, HSR displayed good solubility in mixed EtOH/H2O media and efficient cellular uptake with no cytotoxicity, highlighting its suitability for the detection of the Fe3+ ion and bioimaging applications.
SN - 2470-1343
ER -