Comparative effects of silibinin and deferoxamine on skeletal muscle injury and regeneration in a rat model of compartment syndrome
- 1Orthopaedics and Traumatology Clinic, Sultanbeyli State Hospital
- 2Department of Orthopaedics and Traumatology, Faculty of Medicine, Marmara University Pendik Training and Research Hospital, Marmara University
- 3Department of Pathology, Marmara University Faculty of Medicine, Marmara University Pendik Training and Research Hospital
Abstract
Compartment syndrome (CS) causes ischaemia–reperfusion-related skeletal muscle injury, and adjunct pharmacological strategies may reduce secondary tissue damage. This study compared silibinin and deferoxamine in a rat model of CS. Thirty-six male Sprague–Dawley rats were randomised into six groups (n = 6). CS was induced by applying 140 mmHg external cuff pressure to the right hind limb for 3 h, followed by reperfusion. Animals received vehicle, silibinin (200 mg/kg/day by oral gavage), or deferoxamine (100 mg/kg/day intraperitoneally) until Day 4 or Day 14. CS + vehicle rats received oral distilled water and intraperitoneal 0.9% NaCl without active drug; active treatment groups received route-matched vehicle through the alternative route. Functional performance was assessed using the inclined plane test, and gastrocnemius muscles were examined using hematoxylin-eosin, Masson’s trichrome, Pax7, and Myogenin staining. At Day 14, both treatment groups showed higher inclined plane performance and lower myofiber necrosis and collagen-associated remodeling scores than the CS + vehicle group. Pax7 and Myogenin showed distinct patterns of expression between the Day 4 and Day 14 cohorts. Overall, silibinin and deferoxamine were associated with lower histopathological injury and higher functional performance in the Day 14 groups than in the corresponding Day 4 groups in this experimental CS model, while Pax7 and Myogenin findings indicated differences in myogenic marker expression between the Day 4 and Day 14 cohorts rather than definitive regenerative kinetics.
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BibTeX
@article{koncalioglu2026comparative,
title = {{Comparative effects of silibinin and deferoxamine on skeletal muscle injury and regeneration in a rat model of compartment syndrome}},
author = {Berk Koncalioglu and Çağrı Ata Mutlu and Emine Çeşmecioğlu-Karavin and Batuhan Gencer and Osman Mert Topkar},
journal = {Scientific Reports},
year = {2026},
month = sep,
doi = {10.1038/s41598-026-69079-0},
url = {https://doi.org/10.1038/s41598-026-69079-0},
note = {CC BY},
}
RIS
TY - JOUR
TI - Comparative effects of silibinin and deferoxamine on skeletal muscle injury and regeneration in a rat model of compartment syndrome
AU - Berk Koncalioglu
AU - Çağrı Ata Mutlu
AU - Emine Çeşmecioğlu-Karavin
AU - Batuhan Gencer
AU - Osman Mert Topkar
PY - 2026
DA - 2026/09/01
JO - Scientific Reports
DO - 10.1038/s41598-026-69079-0
UR - https://doi.org/10.1038/s41598-026-69079-0
AB - Compartment syndrome (CS) causes ischaemia–reperfusion-related skeletal muscle injury, and adjunct pharmacological strategies may reduce secondary tissue damage. This study compared silibinin and deferoxamine in a rat model of CS. Thirty-six male Sprague–Dawley rats were randomised into six groups (n = 6). CS was induced by applying 140 mmHg external cuff pressure to the right hind limb for 3 h, followed by reperfusion. Animals received vehicle, silibinin (200 mg/kg/day by oral gavage), or deferoxamine (100 mg/kg/day intraperitoneally) until Day 4 or Day 14. CS + vehicle rats received oral distilled water and intraperitoneal 0.9% NaCl without active drug; active treatment groups received route-matched vehicle through the alternative route. Functional performance was assessed using the inclined plane test, and gastrocnemius muscles were examined using hematoxylin-eosin, Masson’s trichrome, Pax7, and Myogenin staining. At Day 14, both treatment groups showed higher inclined plane performance and lower myofiber necrosis and collagen-associated remodeling scores than the CS + vehicle group. Pax7 and Myogenin showed distinct patterns of expression between the Day 4 and Day 14 cohorts. Overall, silibinin and deferoxamine were associated with lower histopathological injury and higher functional performance in the Day 14 groups than in the corresponding Day 4 groups in this experimental CS model, while Pax7 and Myogenin findings indicated differences in myogenic marker expression between the Day 4 and Day 14 cohorts rather than definitive regenerative kinetics.
SN - 2045-2322
ER -